In Vitro Activity of Octenidine Dihydrochloride-Containing Lozenges against Biofilm-Forming Pathogens of Oral Cavity and Throat
The disruption of the balance in the composition of oral and throat microbiota due to overgrowth of infectious biofilms may lead not only to severe infections, but also to serious, systemic complications resulting in a permanent loss of health or even in the death of the patient. Therefore, a number...
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MDPI AG
2023-02-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/5/2974 |
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| author | Bartłomiej Dudek Justyna Tymińska Patrycja Szymczyk-Ziółkowska Grzegorz Chodaczek Paweł Migdał Joanna Czajkowska Adam Junka |
| author_facet | Bartłomiej Dudek Justyna Tymińska Patrycja Szymczyk-Ziółkowska Grzegorz Chodaczek Paweł Migdał Joanna Czajkowska Adam Junka |
| author_sort | Bartłomiej Dudek |
| collection | DOAJ |
| description | The disruption of the balance in the composition of oral and throat microbiota due to overgrowth of infectious biofilms may lead not only to severe infections, but also to serious, systemic complications resulting in a permanent loss of health or even in the death of the patient. Therefore, a number of hygienic measures are applied to counter-act such a threat, including the provision of locally active antiseptic agents. In this work, the antimicrobial activity of a well-established drug, octenidine dihydrochloride, in a new formulation of lozenges toward the biofilms formed in vitro by <i>Staphylococcus aureus</i>, <i>Streptococcus pyogenes</i>, <i>Candida albicans</i>, <i>Pseudomonas aeruginosa</i> and <i>Aggregatibacter actinomycetemcomitans</i> was assessed. The metabolic activity and quantitative culturing were performed and also scanning electron and confocal microscopies with regard to these biofilms exposed to the activity of octenidine in lozenges vs. a comparator (octenidine dihydrochloride dissolved in liquid). Biofilms were cultured in different experimental settings, including one in which hydroxyapatite served as the biofilm growth surface and using artificial saliva as the biofilm milieu. The obtained results indicated that the tested formulation of octenidine-containing lozenges displayed a high efficacy towards the analyzed biofilms, regardless of the in vitro setting applied. The current work is of a strictly in vitro nature, and the analyses were performed on reference microbial strains and not on the spectrum of clinical strains. Nevertheless, considering the fact that the time of exposition of biofilm to the octenidine released from the lozenge is significantly longer than the contact time of an antiseptic dissolved in liquid also tested in this study, it can be assumed that stable carriers of octenidine may find its broad application in the maintenance of oral and throat hygiene and the eradication of biofilm-based infections. |
| first_indexed | 2024-03-11T07:31:03Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-11T07:31:03Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-bc799918e28c49d288f823d9b3b736c92023-11-17T07:17:38ZengMDPI AGApplied Sciences2076-34172023-02-01135297410.3390/app13052974In Vitro Activity of Octenidine Dihydrochloride-Containing Lozenges against Biofilm-Forming Pathogens of Oral Cavity and ThroatBartłomiej Dudek0Justyna Tymińska1Patrycja Szymczyk-Ziółkowska2Grzegorz Chodaczek3Paweł Migdał4Joanna Czajkowska5Adam Junka6Department of Microbiology, Institute of Genetics and Microbiology, University of Wrocław, 51-148 Wrocław, PolandFaculty of Medicine, Lazarski University, 02-662 Warsaw, PolandCentre for Advanced Manufacturing Technologies (CAMT/FPC), Faculty of Mechanical Engineering, Wrocław University of Science and Technology, 50-371 Wrocław, PolandBioimaging Laboratory, Łukasiewicz Research Network—PORT Polish Center for Technology Development, 54-066 Wrocław, PolandDepartment of Environment Hygiene and Animal Welfare, Wrocław University of Environmental and Life Sciences, 51-630 Wrocław, PolandDepartment of Biochemistry and Molecular Biology, Wrocław University of Environmental and Life Sciences, 50-375 Wrocław, PolandDepartment of Pharmaceutical Microbiology and Parasitology, Unique Application Models Laboratory, Medical University of Wrocław, 50-556 Wrocław, PolandThe disruption of the balance in the composition of oral and throat microbiota due to overgrowth of infectious biofilms may lead not only to severe infections, but also to serious, systemic complications resulting in a permanent loss of health or even in the death of the patient. Therefore, a number of hygienic measures are applied to counter-act such a threat, including the provision of locally active antiseptic agents. In this work, the antimicrobial activity of a well-established drug, octenidine dihydrochloride, in a new formulation of lozenges toward the biofilms formed in vitro by <i>Staphylococcus aureus</i>, <i>Streptococcus pyogenes</i>, <i>Candida albicans</i>, <i>Pseudomonas aeruginosa</i> and <i>Aggregatibacter actinomycetemcomitans</i> was assessed. The metabolic activity and quantitative culturing were performed and also scanning electron and confocal microscopies with regard to these biofilms exposed to the activity of octenidine in lozenges vs. a comparator (octenidine dihydrochloride dissolved in liquid). Biofilms were cultured in different experimental settings, including one in which hydroxyapatite served as the biofilm growth surface and using artificial saliva as the biofilm milieu. The obtained results indicated that the tested formulation of octenidine-containing lozenges displayed a high efficacy towards the analyzed biofilms, regardless of the in vitro setting applied. The current work is of a strictly in vitro nature, and the analyses were performed on reference microbial strains and not on the spectrum of clinical strains. Nevertheless, considering the fact that the time of exposition of biofilm to the octenidine released from the lozenge is significantly longer than the contact time of an antiseptic dissolved in liquid also tested in this study, it can be assumed that stable carriers of octenidine may find its broad application in the maintenance of oral and throat hygiene and the eradication of biofilm-based infections.https://www.mdpi.com/2076-3417/13/5/2974octenidine dihydrochloridelozenge formulationbiofilmsore throatoral cavityeradication of oral and throat infective biofilms |
| spellingShingle | Bartłomiej Dudek Justyna Tymińska Patrycja Szymczyk-Ziółkowska Grzegorz Chodaczek Paweł Migdał Joanna Czajkowska Adam Junka In Vitro Activity of Octenidine Dihydrochloride-Containing Lozenges against Biofilm-Forming Pathogens of Oral Cavity and Throat Applied Sciences octenidine dihydrochloride lozenge formulation biofilm sore throat oral cavity eradication of oral and throat infective biofilms |
| title | In Vitro Activity of Octenidine Dihydrochloride-Containing Lozenges against Biofilm-Forming Pathogens of Oral Cavity and Throat |
| title_full | In Vitro Activity of Octenidine Dihydrochloride-Containing Lozenges against Biofilm-Forming Pathogens of Oral Cavity and Throat |
| title_fullStr | In Vitro Activity of Octenidine Dihydrochloride-Containing Lozenges against Biofilm-Forming Pathogens of Oral Cavity and Throat |
| title_full_unstemmed | In Vitro Activity of Octenidine Dihydrochloride-Containing Lozenges against Biofilm-Forming Pathogens of Oral Cavity and Throat |
| title_short | In Vitro Activity of Octenidine Dihydrochloride-Containing Lozenges against Biofilm-Forming Pathogens of Oral Cavity and Throat |
| title_sort | in vitro activity of octenidine dihydrochloride containing lozenges against biofilm forming pathogens of oral cavity and throat |
| topic | octenidine dihydrochloride lozenge formulation biofilm sore throat oral cavity eradication of oral and throat infective biofilms |
| url | https://www.mdpi.com/2076-3417/13/5/2974 |
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