Biosurfactant derived from probiotic Lactobacillus acidophilus exhibits broad-spectrum antibiofilm activity and inhibits the quorum sensing-regulated virulence
Antimicrobial resistance by pathogenic bacteria has become a global risk to human health in recent years. The most promising approach to combating antimicrobial resistance is to target virulent traits of bacteria. In the present study, a biosurfactant derived from the probiotic strain Lactobacillus...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2023-11-01
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| Series: | Biomolecules & Biomedicine |
| Subjects: | |
| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/9324 |
| _version_ | 1827317533426843648 |
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| author | Mohd Adnan Arif Jamal Siddiqui Emira Noumi Syed Amir Ashraf Amir Mahgoub Awadelkareem Sibte Hadi Mejdi Snoussi Riadh Badraoui Fevzi Bardakci Manojkumar Sachidanandan Mitesh Patel |
| author_facet | Mohd Adnan Arif Jamal Siddiqui Emira Noumi Syed Amir Ashraf Amir Mahgoub Awadelkareem Sibte Hadi Mejdi Snoussi Riadh Badraoui Fevzi Bardakci Manojkumar Sachidanandan Mitesh Patel |
| author_sort | Mohd Adnan |
| collection | DOAJ |
| description | Antimicrobial resistance by pathogenic bacteria has become a global risk to human health in recent years. The most promising approach to combating antimicrobial resistance is to target virulent traits of bacteria. In the present study, a biosurfactant derived from the probiotic strain Lactobacillus acidophilus was tested against three Gram-negative bacteria to evaluate its inhibitory potential on their biofilms, and whether it affected the virulence factors controlled by quorum sensing (QS). A reduction in the virulence factors of Chromobacterium violaceum (violacein production), Serratia marcescens (prodigiosin production) and Pseudomonas aeruginosa (pyocyanin, total protease, LasB elastase and LasA protease production) was observed at different sub-MIC concentrations in a dose-dependent manner. Biofilm development was reduced by 65.76%, 70.64% and 58.12% at the highest sub-MIC levels for C. violaceum, P. aeruginosa and S. marcescens, respectively. Biofilm formation on glass surfaces exhibited significant reduction, with less bacterial aggregation and reduced formation of extracellular polymeric materials. Additionally, swimming motility and exopolysaccharides (EPS) production were shown to be reduced in the presence of the L. acidophilus-derived biosurfactant. Furthermore, molecular docking analysis performed on compounds identified through gas chromatography–mass spectrometry (GC-MS) analysis of QS and biofilm proteins yielded further insights into the mechanism underlying the anti-QS activity. Therefore, the present study has clearly demonstrated that a biosurfactant derived from L. acidophilus can significantly inhibit virulence factors of Gram-negative pathogenic bacteria. This could provide an effective method to inhibit the formation of biofilms and QS in Gram-negative bacteria.
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| first_indexed | 2024-04-24T23:40:27Z |
| format | Article |
| id | doaj.art-bc79fcf962594fc29a3676f47eaefd56 |
| institution | Directory Open Access Journal |
| issn | 2831-0896 2831-090X |
| language | English |
| last_indexed | 2024-04-24T23:40:27Z |
| publishDate | 2023-11-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj.art-bc79fcf962594fc29a3676f47eaefd562024-03-15T13:21:13ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2023-11-0123610.17305/bb.2023.9324Biosurfactant derived from probiotic Lactobacillus acidophilus exhibits broad-spectrum antibiofilm activity and inhibits the quorum sensing-regulated virulenceMohd Adnan0https://orcid.org/0000-0002-7080-6822Arif Jamal Siddiqui1https://orcid.org/0000-0002-6236-0920Emira Noumi2Syed Amir Ashraf3https://orcid.org/0000-0002-5333-9505Amir Mahgoub Awadelkareem4Sibte Hadi5https://orcid.org/0000-0002-2994-3083Mejdi Snoussi6Riadh Badraoui7https://orcid.org/0000-0001-9054-7744Fevzi Bardakci8https://orcid.org/0000-0003-3377-2763Manojkumar Sachidanandan9Mitesh Patel10https://orcid.org/0000-0002-9283-2124Department of Biology, College of Science, University of Ha’il, Ha’il, Saudi ArabiaDepartment of Biology, College of Science, University of Ha’il, Ha’il, Saudi ArabiaDepartment of Biology, College of Science, University of Ha’il, Ha’il, Saudi ArabiaDepartment of Clinical Nutrition, College of Applied Medial Sciences, University of Ha’il, Ha’il, Saudi ArabiaDepartment of Clinical Nutrition, College of Applied Medial Sciences, University of Ha’il, Ha’il, Saudi ArabiaDepartment of Forensic Sciences, Naif Arab University for Security Sciences, Riyadh, Saudi ArabiaDepartment of Biology, College of Science, University of Ha’il, Ha’il, Saudi ArabiaDepartment of Biology, College of Science, University of Ha’il, Ha’il, Saudi ArabiaDepartment of Biology, College of Science, University of Ha’il, Ha’il, Saudi ArabiaDepartment of Oral Radiology, College of Dentistry, University of Ha’il, Ha’il, Saudi ArabiaDepartment of Biotechnology, Parul Institute of Applied Sciences and Centre of Research for Development, Parul University, Vadodara, IndiaAntimicrobial resistance by pathogenic bacteria has become a global risk to human health in recent years. The most promising approach to combating antimicrobial resistance is to target virulent traits of bacteria. In the present study, a biosurfactant derived from the probiotic strain Lactobacillus acidophilus was tested against three Gram-negative bacteria to evaluate its inhibitory potential on their biofilms, and whether it affected the virulence factors controlled by quorum sensing (QS). A reduction in the virulence factors of Chromobacterium violaceum (violacein production), Serratia marcescens (prodigiosin production) and Pseudomonas aeruginosa (pyocyanin, total protease, LasB elastase and LasA protease production) was observed at different sub-MIC concentrations in a dose-dependent manner. Biofilm development was reduced by 65.76%, 70.64% and 58.12% at the highest sub-MIC levels for C. violaceum, P. aeruginosa and S. marcescens, respectively. Biofilm formation on glass surfaces exhibited significant reduction, with less bacterial aggregation and reduced formation of extracellular polymeric materials. Additionally, swimming motility and exopolysaccharides (EPS) production were shown to be reduced in the presence of the L. acidophilus-derived biosurfactant. Furthermore, molecular docking analysis performed on compounds identified through gas chromatography–mass spectrometry (GC-MS) analysis of QS and biofilm proteins yielded further insights into the mechanism underlying the anti-QS activity. Therefore, the present study has clearly demonstrated that a biosurfactant derived from L. acidophilus can significantly inhibit virulence factors of Gram-negative pathogenic bacteria. This could provide an effective method to inhibit the formation of biofilms and QS in Gram-negative bacteria. https://www.bjbms.org/ojs/index.php/bjbms/article/view/9324BiosurfactantLactobacillus acidophilusantibiofilmquorum sensing (QS)virulence |
| spellingShingle | Mohd Adnan Arif Jamal Siddiqui Emira Noumi Syed Amir Ashraf Amir Mahgoub Awadelkareem Sibte Hadi Mejdi Snoussi Riadh Badraoui Fevzi Bardakci Manojkumar Sachidanandan Mitesh Patel Biosurfactant derived from probiotic Lactobacillus acidophilus exhibits broad-spectrum antibiofilm activity and inhibits the quorum sensing-regulated virulence Biomolecules & Biomedicine Biosurfactant Lactobacillus acidophilus antibiofilm quorum sensing (QS) virulence |
| title | Biosurfactant derived from probiotic Lactobacillus acidophilus exhibits broad-spectrum antibiofilm activity and inhibits the quorum sensing-regulated virulence |
| title_full | Biosurfactant derived from probiotic Lactobacillus acidophilus exhibits broad-spectrum antibiofilm activity and inhibits the quorum sensing-regulated virulence |
| title_fullStr | Biosurfactant derived from probiotic Lactobacillus acidophilus exhibits broad-spectrum antibiofilm activity and inhibits the quorum sensing-regulated virulence |
| title_full_unstemmed | Biosurfactant derived from probiotic Lactobacillus acidophilus exhibits broad-spectrum antibiofilm activity and inhibits the quorum sensing-regulated virulence |
| title_short | Biosurfactant derived from probiotic Lactobacillus acidophilus exhibits broad-spectrum antibiofilm activity and inhibits the quorum sensing-regulated virulence |
| title_sort | biosurfactant derived from probiotic lactobacillus acidophilus exhibits broad spectrum antibiofilm activity and inhibits the quorum sensing regulated virulence |
| topic | Biosurfactant Lactobacillus acidophilus antibiofilm quorum sensing (QS) virulence |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/9324 |
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