Influence of extract from shoots of Taxus baccata var. elegantissima on ultrastructure and tubulin cytoskeleton of meristematic cells of Allium cepa L. roots
We investigated the influence of extract from Taxus baccata var. Elegantissima (TbE) shoots in 1:8 dilution, containing paclitaxel in concentration of 81,6 µg/g fresh mass on ultrastructure and tubulin cytoskeleton of meristematic cells of Allium cepa L. root tips. Incubation time 3, 6, 12 and 24 h...
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2014-01-01
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| Series: | Acta Societatis Botanicorum Poloniae |
| Subjects: | |
| Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/626 |
| _version_ | 1819083464516829184 |
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| author | Agnieszka Majewska Mirosława Furmanowa Kazimierz Głowniak Joanna Guzewska Alicja Zobel Mieczysław Kuraś |
| author_facet | Agnieszka Majewska Mirosława Furmanowa Kazimierz Głowniak Joanna Guzewska Alicja Zobel Mieczysław Kuraś |
| author_sort | Agnieszka Majewska |
| collection | DOAJ |
| description | We investigated the influence of extract from Taxus baccata var. Elegantissima (TbE) shoots in 1:8 dilution, containing paclitaxel in concentration of 81,6 µg/g fresh mass on ultrastructure and tubulin cytoskeleton of meristematic cells of Allium cepa L. root tips. Incubation time 3, 6, 12 and 24 h was followed with postincubation in water for 12 and 24 h. During shorter incubation (till 12 h) the surface of the cell nuclei decreased and chromatin became condensed (in comparison to control) but after 24 h the average surface increased and chromatin condensation decreased. In the course of incubation the average size of plastids and vacuoles increased. Moreover, after treatment mitochondria and plastids showed degradation of ultrastructure, which was reversed after 12 h of postincubation. Immunocytochemical assays demonstrated that in the course of incubation in the ThE extract, the tubulin cytoskeleton became partially disorganised. In most interphase cells, cortical microtubules (MTs) lost their oval transverse orientation. The preprophase band (PPB) position in the cell was often asymmetrical. The MTs array of the karyokinetic spindle and phragmoplast was also disturbed. These alterations were completely reversed during postincubation. |
| first_indexed | 2024-12-21T20:32:59Z |
| format | Article |
| id | doaj.art-bc7b5349b3d0477994c1b85d9e410a29 |
| institution | Directory Open Access Journal |
| issn | 2083-9480 |
| language | English |
| last_indexed | 2024-12-21T20:32:59Z |
| publishDate | 2014-01-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Societatis Botanicorum Poloniae |
| spelling | doaj.art-bc7b5349b3d0477994c1b85d9e410a292022-12-21T18:51:11ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802014-01-0171321122110.5586/asbp.2002.025428Influence of extract from shoots of Taxus baccata var. elegantissima on ultrastructure and tubulin cytoskeleton of meristematic cells of Allium cepa L. rootsAgnieszka Majewska0Mirosława Furmanowa1Kazimierz Głowniak2Joanna Guzewska3Alicja Zobel4Mieczysław Kuraś5University of WarsawMedical University of WarsawMedical University of LublinMedical University of WarsawTrent UniversityUniversity of WarsawWe investigated the influence of extract from Taxus baccata var. Elegantissima (TbE) shoots in 1:8 dilution, containing paclitaxel in concentration of 81,6 µg/g fresh mass on ultrastructure and tubulin cytoskeleton of meristematic cells of Allium cepa L. root tips. Incubation time 3, 6, 12 and 24 h was followed with postincubation in water for 12 and 24 h. During shorter incubation (till 12 h) the surface of the cell nuclei decreased and chromatin became condensed (in comparison to control) but after 24 h the average surface increased and chromatin condensation decreased. In the course of incubation the average size of plastids and vacuoles increased. Moreover, after treatment mitochondria and plastids showed degradation of ultrastructure, which was reversed after 12 h of postincubation. Immunocytochemical assays demonstrated that in the course of incubation in the ThE extract, the tubulin cytoskeleton became partially disorganised. In most interphase cells, cortical microtubules (MTs) lost their oval transverse orientation. The preprophase band (PPB) position in the cell was often asymmetrical. The MTs array of the karyokinetic spindle and phragmoplast was also disturbed. These alterations were completely reversed during postincubation.https://pbsociety.org.pl/journals/index.php/asbp/article/view/626Taxus baccata var. Elegantissimaextractpaclitaxelultrastructuretubulin cytoskeleton |
| spellingShingle | Agnieszka Majewska Mirosława Furmanowa Kazimierz Głowniak Joanna Guzewska Alicja Zobel Mieczysław Kuraś Influence of extract from shoots of Taxus baccata var. elegantissima on ultrastructure and tubulin cytoskeleton of meristematic cells of Allium cepa L. roots Acta Societatis Botanicorum Poloniae Taxus baccata var. Elegantissima extract paclitaxel ultrastructure tubulin cytoskeleton |
| title | Influence of extract from shoots of Taxus baccata var. elegantissima on ultrastructure and tubulin cytoskeleton of meristematic cells of Allium cepa L. roots |
| title_full | Influence of extract from shoots of Taxus baccata var. elegantissima on ultrastructure and tubulin cytoskeleton of meristematic cells of Allium cepa L. roots |
| title_fullStr | Influence of extract from shoots of Taxus baccata var. elegantissima on ultrastructure and tubulin cytoskeleton of meristematic cells of Allium cepa L. roots |
| title_full_unstemmed | Influence of extract from shoots of Taxus baccata var. elegantissima on ultrastructure and tubulin cytoskeleton of meristematic cells of Allium cepa L. roots |
| title_short | Influence of extract from shoots of Taxus baccata var. elegantissima on ultrastructure and tubulin cytoskeleton of meristematic cells of Allium cepa L. roots |
| title_sort | influence of extract from shoots of taxus baccata var elegantissima on ultrastructure and tubulin cytoskeleton of meristematic cells of allium cepa l roots |
| topic | Taxus baccata var. Elegantissima extract paclitaxel ultrastructure tubulin cytoskeleton |
| url | https://pbsociety.org.pl/journals/index.php/asbp/article/view/626 |
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