Long undecoded transcript isoform (LUTI) detection in meiotic budding yeast by direct RNA and transcript leader sequencing

Long undecoded transcript isoform (LUTI) detection in meiotic budding yeast by direct RNA and transcript leader sequencing

Summary: LUTIs (Long Undecoded Transcript Isoforms) are 5′-extended and poorly translated mRNAs that can downregulate transcription from promoters more proximal to a gene’s coding sequence (CDS). In this protocol, polyA RNA is extracted from budding yeast cells undergoing highly synchronized meiosis...

Cur síos iomlán

Sonraí bibleagrafaíochta
Príomhchruthaitheoirí: Amy Tresenrider, Minghao Chia, Folkert J. van Werven, Elçin Ünal
Formáid: Alt
Teanga:English
Foilsithe / Cruthaithe: Elsevier 2022-03-01
Sraith:STAR Protocols
Ábhair:
Bioinformatics
Sequence analysis
Genetics
Genomics
Sequencing
Model Organisms
Rochtain ar líne:http://www.sciencedirect.com/science/article/pii/S2666166722000259

Ar líne

http://www.sciencedirect.com/science/article/pii/S2666166722000259

Míreanna comhchosúla