Long undecoded transcript isoform (LUTI) detection in meiotic budding yeast by direct RNA and transcript leader sequencing

Long undecoded transcript isoform (LUTI) detection in meiotic budding yeast by direct RNA and transcript leader sequencing

Summary: LUTIs (Long Undecoded Transcript Isoforms) are 5′-extended and poorly translated mRNAs that can downregulate transcription from promoters more proximal to a gene’s coding sequence (CDS). In this protocol, polyA RNA is extracted from budding yeast cells undergoing highly synchronized meiosis...

पूर्ण विवरण

ग्रंथसूची विवरण
मुख्य लेखकों: Amy Tresenrider, Minghao Chia, Folkert J. van Werven, Elçin Ünal
स्वरूप: लेख
भाषा:English
प्रकाशित: Elsevier 2022-03-01
श्रृंखला:STAR Protocols
विषय:
Bioinformatics
Sequence analysis
Genetics
Genomics
Sequencing
Model Organisms
ऑनलाइन पहुंच:http://www.sciencedirect.com/science/article/pii/S2666166722000259

इंटरनेट

http://www.sciencedirect.com/science/article/pii/S2666166722000259

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