Long undecoded transcript isoform (LUTI) detection in meiotic budding yeast by direct RNA and transcript leader sequencing
Summary: LUTIs (Long Undecoded Transcript Isoforms) are 5′-extended and poorly translated mRNAs that can downregulate transcription from promoters more proximal to a gene’s coding sequence (CDS). In this protocol, polyA RNA is extracted from budding yeast cells undergoing highly synchronized meiosis...
| Main Authors: | , , , |
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| Format: | Article |
| Jezik: | English |
| Izdano: |
Elsevier
2022-03-01
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| Serija: | STAR Protocols |
| Teme: | |
| Online dostop: | http://www.sciencedirect.com/science/article/pii/S2666166722000259 |