Contamination of clinical specimens with MLV-encoding nucleic acids: implications for XMRV and other candidate human retroviruses
<p>Abstract</p> <p>Efforts to assess the prevalence of xenotropic murine leukemia virus-related virus (XMRV) in patients with prostate cancer and chronic fatigue syndrome have relied heavily on PCR-based testing of clinical samples and have yielded widely divergent findings. This w...
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| Format: | Article |
| Language: | English |
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BMC
2010-12-01
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| Series: | Retrovirology |
| Online Access: | http://www.retrovirology.com/content/7/1/112 |
| _version_ | 1818513375551815680 |
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| author | Smith Robert A |
| author_facet | Smith Robert A |
| author_sort | Smith Robert A |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Efforts to assess the prevalence of xenotropic murine leukemia virus-related virus (XMRV) in patients with prostate cancer and chronic fatigue syndrome have relied heavily on PCR-based testing of clinical samples and have yielded widely divergent findings. This week in <it>Retrovirology</it>, reports from four independent research groups illustrate the extreme care needed to exclude DNA or RNA contamination in PCR analyses of XMRV. In addition, phylogenetic evidence suggesting that previously-published XMRV sequences originated from a commonly-used prostate carcinoma cell line (22Rv1) is presented. These findings raise important questions regarding the provenance of XMRV and its potential connection to human disease.</p> |
| first_indexed | 2024-12-11T00:00:26Z |
| format | Article |
| id | doaj.art-bc7c3c78c1fa4dce89a1bb5099fb95cd |
| institution | Directory Open Access Journal |
| issn | 1742-4690 |
| language | English |
| last_indexed | 2024-12-11T00:00:26Z |
| publishDate | 2010-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Retrovirology |
| spelling | doaj.art-bc7c3c78c1fa4dce89a1bb5099fb95cd2022-12-22T01:28:29ZengBMCRetrovirology1742-46902010-12-017111210.1186/1742-4690-7-112Contamination of clinical specimens with MLV-encoding nucleic acids: implications for XMRV and other candidate human retrovirusesSmith Robert A<p>Abstract</p> <p>Efforts to assess the prevalence of xenotropic murine leukemia virus-related virus (XMRV) in patients with prostate cancer and chronic fatigue syndrome have relied heavily on PCR-based testing of clinical samples and have yielded widely divergent findings. This week in <it>Retrovirology</it>, reports from four independent research groups illustrate the extreme care needed to exclude DNA or RNA contamination in PCR analyses of XMRV. In addition, phylogenetic evidence suggesting that previously-published XMRV sequences originated from a commonly-used prostate carcinoma cell line (22Rv1) is presented. These findings raise important questions regarding the provenance of XMRV and its potential connection to human disease.</p>http://www.retrovirology.com/content/7/1/112 |
| spellingShingle | Smith Robert A Contamination of clinical specimens with MLV-encoding nucleic acids: implications for XMRV and other candidate human retroviruses Retrovirology |
| title | Contamination of clinical specimens with MLV-encoding nucleic acids: implications for XMRV and other candidate human retroviruses |
| title_full | Contamination of clinical specimens with MLV-encoding nucleic acids: implications for XMRV and other candidate human retroviruses |
| title_fullStr | Contamination of clinical specimens with MLV-encoding nucleic acids: implications for XMRV and other candidate human retroviruses |
| title_full_unstemmed | Contamination of clinical specimens with MLV-encoding nucleic acids: implications for XMRV and other candidate human retroviruses |
| title_short | Contamination of clinical specimens with MLV-encoding nucleic acids: implications for XMRV and other candidate human retroviruses |
| title_sort | contamination of clinical specimens with mlv encoding nucleic acids implications for xmrv and other candidate human retroviruses |
| url | http://www.retrovirology.com/content/7/1/112 |
| work_keys_str_mv | AT smithroberta contaminationofclinicalspecimenswithmlvencodingnucleicacidsimplicationsforxmrvandothercandidatehumanretroviruses |