Ellagic acid regulates Wnt/?-catenin signaling pathway and CDK8 in HCT 116 and HT 29 colon cancer cells
Colorectal cancer is one of the leading causes of death worldwide. Wnt/?-catenin signalling pathway plays a central role in normal cellular responses, making it a potent target in cancer therapy. Study was taken to assess whether ellagic acid modulates Wnt/?-catenin pathway and CDK8 activity in colo...
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| Format: | Article |
| Language: | English |
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Bangladesh Pharmacological Society
2015-01-01
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| Series: | Bangladesh Journal of Pharmacology |
| Subjects: | |
| Online Access: | https://www.banglajol.info/index.php/BJP/article/view/21068 |
| _version_ | 1818215008103825408 |
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| author | Yang Fang Hong Zhou Jian-Fu Xia Jie-Jun Lin Ri-Zeng Li Da-Qing Yang Mai-Yu Xu Xiao-Yang Li |
| author_facet | Yang Fang Hong Zhou Jian-Fu Xia Jie-Jun Lin Ri-Zeng Li Da-Qing Yang Mai-Yu Xu Xiao-Yang Li |
| author_sort | Yang Fang |
| collection | DOAJ |
| description | Colorectal cancer is one of the leading causes of death worldwide. Wnt/?-catenin signalling pathway plays a central role in normal cellular responses, making it a potent target in cancer therapy. Study was taken to assess whether ellagic acid modulates Wnt/?-catenin pathway and CDK8 activity in colon cancer cells. Effect of ellagic acid on viability of colon cancer cell lines (HT 29 and HCT 116), were assessed by MTT assay and its influence on CDK8, ?-catenin, p-?-catenin, axin1 and 2, survivin, c-Myc and cyclin D1 expressions were determined by western blotting. The levels of survivin, c-Myc and cyclin D1 were also analysed following siCDK8 transfection. Ellagic acid caused significant decrease in viability of HT 29 and HCT 116 cells. Expression of CDK 8, ?-catenin, survivin, c-Myc and cyclin D1were markedly reduced on exposure to ellagic acid. Significant up-regulation in the expression of p-?-catenin, axin1 and 2 were observed. siCDK8 transfection resulted in marked reduction in the expression of survivin, c-Myc and cyclin D1. Ellagic acid was able to effectively reduce cell viability and modulate expressions of Wnt/?-catenin signalling cascade proteins and down regulate the activity and expression of CDK8 in HT 29 and HCT 116 cells. |
| first_indexed | 2024-12-12T06:29:14Z |
| format | Article |
| id | doaj.art-bc7c4f3c38714fccaf857776bb387049 |
| institution | Directory Open Access Journal |
| issn | 1991-0088 |
| language | English |
| last_indexed | 2024-12-12T06:29:14Z |
| publishDate | 2015-01-01 |
| publisher | Bangladesh Pharmacological Society |
| record_format | Article |
| series | Bangladesh Journal of Pharmacology |
| spelling | doaj.art-bc7c4f3c38714fccaf857776bb3870492022-12-22T00:34:40ZengBangladesh Pharmacological SocietyBangladesh Journal of Pharmacology1991-00882015-01-01101Ellagic acid regulates Wnt/?-catenin signaling pathway and CDK8 in HCT 116 and HT 29 colon cancer cellsYang Fang0Hong Zhou1Jian-Fu Xia2Jie-Jun Lin3Ri-Zeng Li4Da-Qing Yang5Mai-Yu Xu6Xiao-Yang Li7Department of Colorectal and Anus Surgery, Wenzhou Central Hospital?Zhejiang Province 325000Department of Colorectal and Anus Surgery, Wenzhou Central Hospital?Zhejiang Province 325000Department of Colorectal and Anus Surgery, Wenzhou Central Hospital?Zhejiang Province 325000Department of Gastroenterology, Wenzhou Central Hospital?Zhejiang Province 325000Department of Colorectal and Anus Surgery, Wenzhou Central Hospital?Zhejiang Province 325000Department of Colorectal and Anus Surgery, Wenzhou Central Hospital?Zhejiang Province 325000Department of Hepatobiliary Surgery, Wenzhou Central Hospital?Zhejiang Province 325000Department of Colorectal and Anus Surgery, Wenzhou Central Hospital?Zhejiang Province 325000Colorectal cancer is one of the leading causes of death worldwide. Wnt/?-catenin signalling pathway plays a central role in normal cellular responses, making it a potent target in cancer therapy. Study was taken to assess whether ellagic acid modulates Wnt/?-catenin pathway and CDK8 activity in colon cancer cells. Effect of ellagic acid on viability of colon cancer cell lines (HT 29 and HCT 116), were assessed by MTT assay and its influence on CDK8, ?-catenin, p-?-catenin, axin1 and 2, survivin, c-Myc and cyclin D1 expressions were determined by western blotting. The levels of survivin, c-Myc and cyclin D1 were also analysed following siCDK8 transfection. Ellagic acid caused significant decrease in viability of HT 29 and HCT 116 cells. Expression of CDK 8, ?-catenin, survivin, c-Myc and cyclin D1were markedly reduced on exposure to ellagic acid. Significant up-regulation in the expression of p-?-catenin, axin1 and 2 were observed. siCDK8 transfection resulted in marked reduction in the expression of survivin, c-Myc and cyclin D1. Ellagic acid was able to effectively reduce cell viability and modulate expressions of Wnt/?-catenin signalling cascade proteins and down regulate the activity and expression of CDK8 in HT 29 and HCT 116 cells.https://www.banglajol.info/index.php/BJP/article/view/21068CDK8Colon cancerEllagic acidWnt/?-catenin |
| spellingShingle | Yang Fang Hong Zhou Jian-Fu Xia Jie-Jun Lin Ri-Zeng Li Da-Qing Yang Mai-Yu Xu Xiao-Yang Li Ellagic acid regulates Wnt/?-catenin signaling pathway and CDK8 in HCT 116 and HT 29 colon cancer cells Bangladesh Journal of Pharmacology CDK8 Colon cancer Ellagic acid Wnt/?-catenin |
| title | Ellagic acid regulates Wnt/?-catenin signaling pathway and CDK8 in HCT 116 and HT 29 colon cancer cells |
| title_full | Ellagic acid regulates Wnt/?-catenin signaling pathway and CDK8 in HCT 116 and HT 29 colon cancer cells |
| title_fullStr | Ellagic acid regulates Wnt/?-catenin signaling pathway and CDK8 in HCT 116 and HT 29 colon cancer cells |
| title_full_unstemmed | Ellagic acid regulates Wnt/?-catenin signaling pathway and CDK8 in HCT 116 and HT 29 colon cancer cells |
| title_short | Ellagic acid regulates Wnt/?-catenin signaling pathway and CDK8 in HCT 116 and HT 29 colon cancer cells |
| title_sort | ellagic acid regulates wnt catenin signaling pathway and cdk8 in hct 116 and ht 29 colon cancer cells |
| topic | CDK8 Colon cancer Ellagic acid Wnt/?-catenin |
| url | https://www.banglajol.info/index.php/BJP/article/view/21068 |
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