A Simple and Efficient In Vivo non-viral RNA Transfection Method For Labeling the Whole Axonal Tree of Individual Adult Long-Range Projection Neurons

A Simple and Efficient In Vivo non-viral RNA Transfection Method For Labeling the Whole Axonal Tree of Individual Adult Long-Range Projection Neurons

We report a highly efficient, simple and non-infective method for labeling individual long-range projection neurons in a specific location with enough sparseness and intensity to allow complete and unambiguous reconstructions of their entire axonal tree. The method is based on the in vivo transfecti...

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Bibliographic Details
Main Authors: César ePorrero, Javier eRodríguez-Moreno, José I Quetglas, Cristian eSmerdou, Takahiro eFuruta, Francisco eClasca
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-03-01
Series:Frontiers in Neuroanatomy
Subjects:
Electroporation
connectomics
Sindbis
Axon tracing
Non-viral RNA transfection
Online Access:http://journal.frontiersin.org/Journal/10.3389/fnana.2016.00027/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fnana.2016.00027/full

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