Efficient and Scalable Precision Genome Editing in <italic toggle="yes">Staphylococcus aureus</italic> through Conditional Recombineering and CRISPR/Cas9-Mediated Counterselection

Efficient and Scalable Precision Genome Editing in <italic toggle="yes">Staphylococcus aureus</italic> through Conditional Recombineering and CRISPR/Cas9-Mediated Counterselection

ABSTRACT Staphylococcus aureus is an important human pathogen, but studies of the organism have suffered from the lack of a robust tool set for its genetic and genomic manipulation. Here we report the development of a system for the facile and high-throughput genomic engineering of S. aureus using s...

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Bibliographic Details
Main Authors: Kelsi Penewit, Elizabeth A. Holmes, Kathryn McLean, Mingxin Ren, Adam Waalkes, Stephen J. Salipante
Format: Article
Language:English
Published: American Society for Microbiology 2018-03-01
Series:mBio
Subjects:
CRISPR
Cas9
Staphylococcus aureus
genetic engineering
genome editing
mutS
Online Access:https://journals.asm.org/doi/10.1128/mBio.00067-18

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