2A and 2A-like Sequences: Distribution in Different Virus Species and Applications in Biotechnology
2A is an oligopeptide sequence that mediates a ribosome “skipping” effect and can mediate a co-translation cleavage of polyproteins. These sequences are widely distributed from insect to mammalian viruses and could act by accelerating adaptive capacity. These sequences have been used in many heterol...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/13/11/2160 |
| _version_ | 1797508225211301888 |
|---|---|
| author | Juliana G. S. de Lima Daniel C. F. Lanza |
| author_facet | Juliana G. S. de Lima Daniel C. F. Lanza |
| author_sort | Juliana G. S. de Lima |
| collection | DOAJ |
| description | 2A is an oligopeptide sequence that mediates a ribosome “skipping” effect and can mediate a co-translation cleavage of polyproteins. These sequences are widely distributed from insect to mammalian viruses and could act by accelerating adaptive capacity. These sequences have been used in many heterologous co-expression systems because they are versatile tools for cleaving proteins of biotechnological interest. In this work, we review and update the occurrence of 2A/2A-like sequences in different groups of viruses by screening the sequences available in the National Center for Biotechnology Information database. Interestingly, we reported the occurrence of 2A-like for the first time in 69 sequences. Among these, 62 corresponded to positive single-stranded RNA species, six to double stranded RNA viruses, and one to a negative-sense single-stranded RNA virus. The importance of these sequences for viral evolution and their potential in biotechnological applications are also discussed. |
| first_indexed | 2024-03-10T04:59:29Z |
| format | Article |
| id | doaj.art-bc84e4a643474d03a09e0e79af9b0838 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T04:59:29Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-bc84e4a643474d03a09e0e79af9b08382023-11-23T01:55:50ZengMDPI AGViruses1999-49152021-10-011311216010.3390/v131121602A and 2A-like Sequences: Distribution in Different Virus Species and Applications in BiotechnologyJuliana G. S. de Lima0Daniel C. F. Lanza1Applied Molecular Biology Lab—LAPLIC, Department of Biochemistry, Federal University of Rio Grande do Norte, Natal 59064-720, BrazilApplied Molecular Biology Lab—LAPLIC, Department of Biochemistry, Federal University of Rio Grande do Norte, Natal 59064-720, Brazil2A is an oligopeptide sequence that mediates a ribosome “skipping” effect and can mediate a co-translation cleavage of polyproteins. These sequences are widely distributed from insect to mammalian viruses and could act by accelerating adaptive capacity. These sequences have been used in many heterologous co-expression systems because they are versatile tools for cleaving proteins of biotechnological interest. In this work, we review and update the occurrence of 2A/2A-like sequences in different groups of viruses by screening the sequences available in the National Center for Biotechnology Information database. Interestingly, we reported the occurrence of 2A-like for the first time in 69 sequences. Among these, 62 corresponded to positive single-stranded RNA species, six to double stranded RNA viruses, and one to a negative-sense single-stranded RNA virus. The importance of these sequences for viral evolution and their potential in biotechnological applications are also discussed.https://www.mdpi.com/1999-4915/13/11/21602A peptidedouble-stranded RNA viruspositive-sense single-stranded RNA virusTotiviridaePicornaviridae |
| spellingShingle | Juliana G. S. de Lima Daniel C. F. Lanza 2A and 2A-like Sequences: Distribution in Different Virus Species and Applications in Biotechnology Viruses 2A peptide double-stranded RNA virus positive-sense single-stranded RNA virus Totiviridae Picornaviridae |
| title | 2A and 2A-like Sequences: Distribution in Different Virus Species and Applications in Biotechnology |
| title_full | 2A and 2A-like Sequences: Distribution in Different Virus Species and Applications in Biotechnology |
| title_fullStr | 2A and 2A-like Sequences: Distribution in Different Virus Species and Applications in Biotechnology |
| title_full_unstemmed | 2A and 2A-like Sequences: Distribution in Different Virus Species and Applications in Biotechnology |
| title_short | 2A and 2A-like Sequences: Distribution in Different Virus Species and Applications in Biotechnology |
| title_sort | 2a and 2a like sequences distribution in different virus species and applications in biotechnology |
| topic | 2A peptide double-stranded RNA virus positive-sense single-stranded RNA virus Totiviridae Picornaviridae |
| url | https://www.mdpi.com/1999-4915/13/11/2160 |
| work_keys_str_mv | AT julianagsdelima 2aand2alikesequencesdistributionindifferentvirusspeciesandapplicationsinbiotechnology AT danielcflanza 2aand2alikesequencesdistributionindifferentvirusspeciesandapplicationsinbiotechnology |