Synthesis and Characterization of Polyaniline/Graphene Composite Nanofiber and Its Application as an Electrochemical DNA Biosensor for the Detection of Mycobacterium tuberculosis
This article describes chemically modified polyaniline and graphene (PANI/GP) composite nanofibers prepared by self-assembly process using oxidative polymerization of aniline monomer and graphene in the presence of a solution containing poly(methyl vinyl ether-alt-maleic acid) (PMVEA). Characterizat...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2017-12-01
|
| Series: | Sensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8220/17/12/2789 |
| _version_ | 1828136125716234240 |
|---|---|
| author | Fatimah Syahidah Mohamad Mohd Hazani Mat Zaid Jaafar Abdullah Ruzniza Mohd Zawawi Hong Ngee Lim Yusran Sulaiman Norizah Abdul Rahman |
| author_facet | Fatimah Syahidah Mohamad Mohd Hazani Mat Zaid Jaafar Abdullah Ruzniza Mohd Zawawi Hong Ngee Lim Yusran Sulaiman Norizah Abdul Rahman |
| author_sort | Fatimah Syahidah Mohamad |
| collection | DOAJ |
| description | This article describes chemically modified polyaniline and graphene (PANI/GP) composite nanofibers prepared by self-assembly process using oxidative polymerization of aniline monomer and graphene in the presence of a solution containing poly(methyl vinyl ether-alt-maleic acid) (PMVEA). Characterization of the composite nanofibers was carried out by Fourier transform infrared (FTIR) and Raman spectroscopy, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). SEM images revealed the size of the PANI nanofibers ranged from 90 to 360 nm in diameter and was greatly influenced by the proportion of PMVEA and graphene. The composite nanofibers with an immobilized DNA probe were used for the detection of Mycobacterium tuberculosis by using an electrochemical technique. A photochemical indicator, methylene blue (MB) was used to monitor the hybridization of target DNA by using differential pulse voltammetry (DPV) method. The detection range of DNA biosensor was obtained from of 10−6–10−9 M with the detection limit of 7.853 × 10−7 M under optimum conditions. The results show that the composite nanofibers have a great potential in a range of applications for DNA sensors. |
| first_indexed | 2024-04-11T17:59:01Z |
| format | Article |
| id | doaj.art-bc8528e9d9de4b7fba76ebb1b082b12c |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T17:59:01Z |
| publishDate | 2017-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-bc8528e9d9de4b7fba76ebb1b082b12c2022-12-22T04:10:35ZengMDPI AGSensors1424-82202017-12-011712278910.3390/s17122789s17122789Synthesis and Characterization of Polyaniline/Graphene Composite Nanofiber and Its Application as an Electrochemical DNA Biosensor for the Detection of Mycobacterium tuberculosisFatimah Syahidah Mohamad0Mohd Hazani Mat Zaid1Jaafar Abdullah2Ruzniza Mohd Zawawi3Hong Ngee Lim4Yusran Sulaiman5Norizah Abdul Rahman6Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, MalaysiaDepartment of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, MalaysiaDepartment of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, MalaysiaDepartment of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, MalaysiaDepartment of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, MalaysiaDepartment of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, MalaysiaDepartment of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, MalaysiaThis article describes chemically modified polyaniline and graphene (PANI/GP) composite nanofibers prepared by self-assembly process using oxidative polymerization of aniline monomer and graphene in the presence of a solution containing poly(methyl vinyl ether-alt-maleic acid) (PMVEA). Characterization of the composite nanofibers was carried out by Fourier transform infrared (FTIR) and Raman spectroscopy, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). SEM images revealed the size of the PANI nanofibers ranged from 90 to 360 nm in diameter and was greatly influenced by the proportion of PMVEA and graphene. The composite nanofibers with an immobilized DNA probe were used for the detection of Mycobacterium tuberculosis by using an electrochemical technique. A photochemical indicator, methylene blue (MB) was used to monitor the hybridization of target DNA by using differential pulse voltammetry (DPV) method. The detection range of DNA biosensor was obtained from of 10−6–10−9 M with the detection limit of 7.853 × 10−7 M under optimum conditions. The results show that the composite nanofibers have a great potential in a range of applications for DNA sensors.https://www.mdpi.com/1424-8220/17/12/2789Mycobacterium tuberculosispolyanilinegraphenenanofibersbiosensors |
| spellingShingle | Fatimah Syahidah Mohamad Mohd Hazani Mat Zaid Jaafar Abdullah Ruzniza Mohd Zawawi Hong Ngee Lim Yusran Sulaiman Norizah Abdul Rahman Synthesis and Characterization of Polyaniline/Graphene Composite Nanofiber and Its Application as an Electrochemical DNA Biosensor for the Detection of Mycobacterium tuberculosis Sensors Mycobacterium tuberculosis polyaniline graphene nanofibers biosensors |
| title | Synthesis and Characterization of Polyaniline/Graphene Composite Nanofiber and Its Application as an Electrochemical DNA Biosensor for the Detection of Mycobacterium tuberculosis |
| title_full | Synthesis and Characterization of Polyaniline/Graphene Composite Nanofiber and Its Application as an Electrochemical DNA Biosensor for the Detection of Mycobacterium tuberculosis |
| title_fullStr | Synthesis and Characterization of Polyaniline/Graphene Composite Nanofiber and Its Application as an Electrochemical DNA Biosensor for the Detection of Mycobacterium tuberculosis |
| title_full_unstemmed | Synthesis and Characterization of Polyaniline/Graphene Composite Nanofiber and Its Application as an Electrochemical DNA Biosensor for the Detection of Mycobacterium tuberculosis |
| title_short | Synthesis and Characterization of Polyaniline/Graphene Composite Nanofiber and Its Application as an Electrochemical DNA Biosensor for the Detection of Mycobacterium tuberculosis |
| title_sort | synthesis and characterization of polyaniline graphene composite nanofiber and its application as an electrochemical dna biosensor for the detection of mycobacterium tuberculosis |
| topic | Mycobacterium tuberculosis polyaniline graphene nanofibers biosensors |
| url | https://www.mdpi.com/1424-8220/17/12/2789 |
| work_keys_str_mv | AT fatimahsyahidahmohamad synthesisandcharacterizationofpolyanilinegraphenecompositenanofiberanditsapplicationasanelectrochemicaldnabiosensorforthedetectionofmycobacteriumtuberculosis AT mohdhazanimatzaid synthesisandcharacterizationofpolyanilinegraphenecompositenanofiberanditsapplicationasanelectrochemicaldnabiosensorforthedetectionofmycobacteriumtuberculosis AT jaafarabdullah synthesisandcharacterizationofpolyanilinegraphenecompositenanofiberanditsapplicationasanelectrochemicaldnabiosensorforthedetectionofmycobacteriumtuberculosis AT ruznizamohdzawawi synthesisandcharacterizationofpolyanilinegraphenecompositenanofiberanditsapplicationasanelectrochemicaldnabiosensorforthedetectionofmycobacteriumtuberculosis AT hongngeelim synthesisandcharacterizationofpolyanilinegraphenecompositenanofiberanditsapplicationasanelectrochemicaldnabiosensorforthedetectionofmycobacteriumtuberculosis AT yusransulaiman synthesisandcharacterizationofpolyanilinegraphenecompositenanofiberanditsapplicationasanelectrochemicaldnabiosensorforthedetectionofmycobacteriumtuberculosis AT norizahabdulrahman synthesisandcharacterizationofpolyanilinegraphenecompositenanofiberanditsapplicationasanelectrochemicaldnabiosensorforthedetectionofmycobacteriumtuberculosis |