Validation of a method for ethanol analysis in biological and non-biological samples and its toxicological application
A simple, cost-effective and fast headspace gas chromatography method coupled with flame ionization detection (HS-GC/FID) for determination of ethanol was developed and validated for clinical and forensic toxicology purposes. HS-GC/FID is often used for alcohol determination in different biological...
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| Format: | Article |
| Language: | English |
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Association of Chemical Engineers of Serbia
2021-01-01
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| Series: | Hemijska Industrija |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0367-598X/2021/0367-598X2100016K.pdf |
| _version_ | 1818779068546416640 |
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| author | Kostić Emilija Vujović Maja Milosavljević Biljana |
| author_facet | Kostić Emilija Vujović Maja Milosavljević Biljana |
| author_sort | Kostić Emilija |
| collection | DOAJ |
| description | A simple, cost-effective and fast headspace gas chromatography method coupled with flame ionization detection (HS-GC/FID) for determination of ethanol was developed and validated for clinical and forensic toxicology purposes. HS-GC/FID is often used for alcohol determination in different biological and non-biological samples. The calibration dependence of the method was linear in the range from 0.15 to 4.00 g dm-3 (r2=0.999) with adequate accuracy (99–106 %) and precision. The limit of detection (LOD) was 0.006 g dm-3. The method was quantitative (LOQ) above 0.020 g dm-3. The new method was successfully used for determination of ethanol in biological samples of intoxicated patients, car accidents participants, participants in criminal acts, and postmortem samples, non-biological samples such as alcoholic beverages, alcohol-based herbal preparations, cosmetic preparations, etc. This method is easy to perform, making it suitable not only for the routine applications in clinical biochemistry and forensic laboratories, but also in different fields of industry (e.g. for pharmaceutical preparations, cosmetics, dietary supplements, etc.). Some of the applications for ethanol determination in different samples related to various clinical-forensic cases are presented. |
| first_indexed | 2024-12-18T11:54:44Z |
| format | Article |
| id | doaj.art-bc858af04f87455ab7166ebf7616fd69 |
| institution | Directory Open Access Journal |
| issn | 0367-598X 2217-7426 |
| language | English |
| last_indexed | 2024-12-18T11:54:44Z |
| publishDate | 2021-01-01 |
| publisher | Association of Chemical Engineers of Serbia |
| record_format | Article |
| series | Hemijska Industrija |
| spelling | doaj.art-bc858af04f87455ab7166ebf7616fd692022-12-21T21:09:06ZengAssociation of Chemical Engineers of SerbiaHemijska Industrija0367-598X2217-74262021-01-0175317518310.2298/HEMIND201201016K0367-598X2100016KValidation of a method for ethanol analysis in biological and non-biological samples and its toxicological applicationKostić Emilija0https://orcid.org/0000-0002-4033-6348Vujović Maja1https://orcid.org/0000-0002-5728-4441Milosavljević Biljana2https://orcid.org/0000-0002-8800-2515Faculty of Medicine, University of Niš, SerbiaFaculty of Medicine, University of Niš, Serbia + Institute of Forensic Medicine Niš, SerbiaInstitute of Forensic Medicine Niš, SerbiaA simple, cost-effective and fast headspace gas chromatography method coupled with flame ionization detection (HS-GC/FID) for determination of ethanol was developed and validated for clinical and forensic toxicology purposes. HS-GC/FID is often used for alcohol determination in different biological and non-biological samples. The calibration dependence of the method was linear in the range from 0.15 to 4.00 g dm-3 (r2=0.999) with adequate accuracy (99–106 %) and precision. The limit of detection (LOD) was 0.006 g dm-3. The method was quantitative (LOQ) above 0.020 g dm-3. The new method was successfully used for determination of ethanol in biological samples of intoxicated patients, car accidents participants, participants in criminal acts, and postmortem samples, non-biological samples such as alcoholic beverages, alcohol-based herbal preparations, cosmetic preparations, etc. This method is easy to perform, making it suitable not only for the routine applications in clinical biochemistry and forensic laboratories, but also in different fields of industry (e.g. for pharmaceutical preparations, cosmetics, dietary supplements, etc.). Some of the applications for ethanol determination in different samples related to various clinical-forensic cases are presented.http://www.doiserbia.nb.rs/img/doi/0367-598X/2021/0367-598X2100016K.pdfheadspacegas chromatographyalcoholic beveragescosmetics |
| spellingShingle | Kostić Emilija Vujović Maja Milosavljević Biljana Validation of a method for ethanol analysis in biological and non-biological samples and its toxicological application Hemijska Industrija headspace gas chromatography alcoholic beverages cosmetics |
| title | Validation of a method for ethanol analysis in biological and non-biological samples and its toxicological application |
| title_full | Validation of a method for ethanol analysis in biological and non-biological samples and its toxicological application |
| title_fullStr | Validation of a method for ethanol analysis in biological and non-biological samples and its toxicological application |
| title_full_unstemmed | Validation of a method for ethanol analysis in biological and non-biological samples and its toxicological application |
| title_short | Validation of a method for ethanol analysis in biological and non-biological samples and its toxicological application |
| title_sort | validation of a method for ethanol analysis in biological and non biological samples and its toxicological application |
| topic | headspace gas chromatography alcoholic beverages cosmetics |
| url | http://www.doiserbia.nb.rs/img/doi/0367-598X/2021/0367-598X2100016K.pdf |
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