A 2D-proteomic analysis identifies proteins differentially regulated by two different dengue virus serotypes
Abstract The mosquito transmitted dengue virus (DENV) is a major public health problem in many tropical and sub-tropical countries around the world. Both vaccine development and drug development are complex as the species Dengue virus consist of four distinct viruses (DENV 1 to DENV 4) each of which...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-04-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-57930-1 |
| _version_ | 1797209367118872576 |
|---|---|
| author | Chanida Chumchanchira Suwipa Ramphan Atchara Paemanee Sittiruk Roytrakul Pathrapol Lithanatudom Duncan R. Smith |
| author_facet | Chanida Chumchanchira Suwipa Ramphan Atchara Paemanee Sittiruk Roytrakul Pathrapol Lithanatudom Duncan R. Smith |
| author_sort | Chanida Chumchanchira |
| collection | DOAJ |
| description | Abstract The mosquito transmitted dengue virus (DENV) is a major public health problem in many tropical and sub-tropical countries around the world. Both vaccine development and drug development are complex as the species Dengue virus consist of four distinct viruses (DENV 1 to DENV 4) each of which is composed of multiple lineages and strains. To understand the interaction of DENV with the host cell machinery, several studies have undertaken in vitro proteomic analysis of different cell lines infected with DENV. Invariably, these studies have utilized DENV 2. In this study we sought to define proteins that are differentially regulated by two different DENVs, DENV 2 and DENV 4. A 2-dimensional proteomic analysis identified some 300 protein spots, of which only 11 showed differential expression by both DENVs. Of these, only six were coordinately regulated. One protein, prohibitin 1 (PHB1) was downregulated by infection with both DENVs. Overexpression of PHB1 increased DENV protein expression, level of infection and genome copy number. DENV E protein colocalized with PHB, and there was a direct interaction between DENV 2 E protein and PHB1, but not between DENV 4 E protein and PHB1. The low number of proteins showing coordinate regulation after infection by different DENVs is a cause for concern, particularly in determining new druggable targets, and suggests that studies should routinely investigate multiple DENVs. |
| first_indexed | 2024-04-24T09:53:34Z |
| format | Article |
| id | doaj.art-bc89a07c9dfb43dfa70f420efa4eff2c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T09:53:34Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-bc89a07c9dfb43dfa70f420efa4eff2c2024-04-14T11:17:00ZengNature PortfolioScientific Reports2045-23222024-04-0114111210.1038/s41598-024-57930-1A 2D-proteomic analysis identifies proteins differentially regulated by two different dengue virus serotypesChanida Chumchanchira0Suwipa Ramphan1Atchara Paemanee2Sittiruk Roytrakul3Pathrapol Lithanatudom4Duncan R. Smith5PhD Degree Program in Biology (International Program), Department of Biology, Faculty of Science, Chiang Mai UniversityInstitute of Molecular Biosciences, Mahidol UniversityNational Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development AgencyNational Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development AgencyDepartment of Biology, Faculty of Science, Chiang Mai UniversityInstitute of Molecular Biosciences, Mahidol UniversityAbstract The mosquito transmitted dengue virus (DENV) is a major public health problem in many tropical and sub-tropical countries around the world. Both vaccine development and drug development are complex as the species Dengue virus consist of four distinct viruses (DENV 1 to DENV 4) each of which is composed of multiple lineages and strains. To understand the interaction of DENV with the host cell machinery, several studies have undertaken in vitro proteomic analysis of different cell lines infected with DENV. Invariably, these studies have utilized DENV 2. In this study we sought to define proteins that are differentially regulated by two different DENVs, DENV 2 and DENV 4. A 2-dimensional proteomic analysis identified some 300 protein spots, of which only 11 showed differential expression by both DENVs. Of these, only six were coordinately regulated. One protein, prohibitin 1 (PHB1) was downregulated by infection with both DENVs. Overexpression of PHB1 increased DENV protein expression, level of infection and genome copy number. DENV E protein colocalized with PHB, and there was a direct interaction between DENV 2 E protein and PHB1, but not between DENV 4 E protein and PHB1. The low number of proteins showing coordinate regulation after infection by different DENVs is a cause for concern, particularly in determining new druggable targets, and suggests that studies should routinely investigate multiple DENVs.https://doi.org/10.1038/s41598-024-57930-1 |
| spellingShingle | Chanida Chumchanchira Suwipa Ramphan Atchara Paemanee Sittiruk Roytrakul Pathrapol Lithanatudom Duncan R. Smith A 2D-proteomic analysis identifies proteins differentially regulated by two different dengue virus serotypes Scientific Reports |
| title | A 2D-proteomic analysis identifies proteins differentially regulated by two different dengue virus serotypes |
| title_full | A 2D-proteomic analysis identifies proteins differentially regulated by two different dengue virus serotypes |
| title_fullStr | A 2D-proteomic analysis identifies proteins differentially regulated by two different dengue virus serotypes |
| title_full_unstemmed | A 2D-proteomic analysis identifies proteins differentially regulated by two different dengue virus serotypes |
| title_short | A 2D-proteomic analysis identifies proteins differentially regulated by two different dengue virus serotypes |
| title_sort | 2d proteomic analysis identifies proteins differentially regulated by two different dengue virus serotypes |
| url | https://doi.org/10.1038/s41598-024-57930-1 |
| work_keys_str_mv | AT chanidachumchanchira a2dproteomicanalysisidentifiesproteinsdifferentiallyregulatedbytwodifferentdenguevirusserotypes AT suwiparamphan a2dproteomicanalysisidentifiesproteinsdifferentiallyregulatedbytwodifferentdenguevirusserotypes AT atcharapaemanee a2dproteomicanalysisidentifiesproteinsdifferentiallyregulatedbytwodifferentdenguevirusserotypes AT sittirukroytrakul a2dproteomicanalysisidentifiesproteinsdifferentiallyregulatedbytwodifferentdenguevirusserotypes AT pathrapollithanatudom a2dproteomicanalysisidentifiesproteinsdifferentiallyregulatedbytwodifferentdenguevirusserotypes AT duncanrsmith a2dproteomicanalysisidentifiesproteinsdifferentiallyregulatedbytwodifferentdenguevirusserotypes AT chanidachumchanchira 2dproteomicanalysisidentifiesproteinsdifferentiallyregulatedbytwodifferentdenguevirusserotypes AT suwiparamphan 2dproteomicanalysisidentifiesproteinsdifferentiallyregulatedbytwodifferentdenguevirusserotypes AT atcharapaemanee 2dproteomicanalysisidentifiesproteinsdifferentiallyregulatedbytwodifferentdenguevirusserotypes AT sittirukroytrakul 2dproteomicanalysisidentifiesproteinsdifferentiallyregulatedbytwodifferentdenguevirusserotypes AT pathrapollithanatudom 2dproteomicanalysisidentifiesproteinsdifferentiallyregulatedbytwodifferentdenguevirusserotypes AT duncanrsmith 2dproteomicanalysisidentifiesproteinsdifferentiallyregulatedbytwodifferentdenguevirusserotypes |