Study on efficient metabolism mechanism of starch in the intestine of carnivorous fish: A case of Monopterus albus
An efficient metabolism of mammals in the intestine is a crucial factor in their dietary starch utilization. Swamp eel (Monopterus albus), a carnivorous fish, has shown a remarkable ability to utilize dietary starch efficiently. However, the specific mechanisms underlying this efficient starch metab...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423003344 |
| _version_ | 1827582090279911424 |
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| author | Wenlu Zhu Lairong Qiu Yalin Niu Min Pan Xiaorui Chen Qiubai Zhou Chuanqi Yu |
| author_facet | Wenlu Zhu Lairong Qiu Yalin Niu Min Pan Xiaorui Chen Qiubai Zhou Chuanqi Yu |
| author_sort | Wenlu Zhu |
| collection | DOAJ |
| description | An efficient metabolism of mammals in the intestine is a crucial factor in their dietary starch utilization. Swamp eel (Monopterus albus), a carnivorous fish, has shown a remarkable ability to utilize dietary starch efficiently. However, the specific mechanisms underlying this efficient starch metabolism in the intestine remain poorly understood. Thus, we conducted a study in which juvenile swamp eels (average weight: 12.10 ± 0.03 g) were fed diets with two different starch levels (20 % -NF and 32 % -HF) for a period of 56 days. Subsequently, the intestine and digesta were extracted for analysis. This study showed that there was no significant variety on the activity of digestive enzyme and histological structure in anterior intestine of fish between HF group and NF group. However, HF diets down-regulated the expression of all differentially expressed genes of glycine--tRNA ligase concerned to damage in the most significantly enriched GO function of glycyl-tRNA aminoacylation. Besides, the HF diets significantly increased the genus of Cetobacterium with the function of producing acetic acid. Consistently, the HF diets significantly increased the concentration of short chain fatty acids (SCFA), particularly acetic acid, in the posterior intestine digesta of swamp eel. In summary, our study argued that the enhanced efficiency in utilizing dietary starch by the swamp eel might be attributed to the increased abundance of Cetobacterium and the subsequent production of acetic acid in the posterior intestine. |
| first_indexed | 2024-03-08T22:46:34Z |
| format | Article |
| id | doaj.art-bc89cef6d56c4ef2baa3a0449849a352 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-08T22:46:34Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-bc89cef6d56c4ef2baa3a0449849a3522023-12-17T06:39:45ZengElsevierAquaculture Reports2352-51342023-12-0133101795Study on efficient metabolism mechanism of starch in the intestine of carnivorous fish: A case of Monopterus albusWenlu Zhu0Lairong Qiu1Yalin Niu2Min Pan3Xiaorui Chen4Qiubai Zhou5Chuanqi Yu6College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China; Key Laboratory of Featured Hydrobios Nutritional Physiology and Healthy Breeding, Nanchang 330045, ChinaCollege of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China; Key Laboratory of Featured Hydrobios Nutritional Physiology and Healthy Breeding, Nanchang 330045, ChinaCollege of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China; Key Laboratory of Featured Hydrobios Nutritional Physiology and Healthy Breeding, Nanchang 330045, ChinaCollege of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China; Key Laboratory of Featured Hydrobios Nutritional Physiology and Healthy Breeding, Nanchang 330045, ChinaCollege of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China; Key Laboratory of Featured Hydrobios Nutritional Physiology and Healthy Breeding, Nanchang 330045, ChinaCollege of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China; Key Laboratory of Featured Hydrobios Nutritional Physiology and Healthy Breeding, Nanchang 330045, China; Corresponding authors at: College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China; Key Laboratory of Featured Hydrobios Nutritional Physiology and Healthy Breeding, Nanchang 330045, China; Corresponding authors at: College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.An efficient metabolism of mammals in the intestine is a crucial factor in their dietary starch utilization. Swamp eel (Monopterus albus), a carnivorous fish, has shown a remarkable ability to utilize dietary starch efficiently. However, the specific mechanisms underlying this efficient starch metabolism in the intestine remain poorly understood. Thus, we conducted a study in which juvenile swamp eels (average weight: 12.10 ± 0.03 g) were fed diets with two different starch levels (20 % -NF and 32 % -HF) for a period of 56 days. Subsequently, the intestine and digesta were extracted for analysis. This study showed that there was no significant variety on the activity of digestive enzyme and histological structure in anterior intestine of fish between HF group and NF group. However, HF diets down-regulated the expression of all differentially expressed genes of glycine--tRNA ligase concerned to damage in the most significantly enriched GO function of glycyl-tRNA aminoacylation. Besides, the HF diets significantly increased the genus of Cetobacterium with the function of producing acetic acid. Consistently, the HF diets significantly increased the concentration of short chain fatty acids (SCFA), particularly acetic acid, in the posterior intestine digesta of swamp eel. In summary, our study argued that the enhanced efficiency in utilizing dietary starch by the swamp eel might be attributed to the increased abundance of Cetobacterium and the subsequent production of acetic acid in the posterior intestine.http://www.sciencedirect.com/science/article/pii/S2352513423003344Freshwater fishSCFAMicrofloraOmics |
| spellingShingle | Wenlu Zhu Lairong Qiu Yalin Niu Min Pan Xiaorui Chen Qiubai Zhou Chuanqi Yu Study on efficient metabolism mechanism of starch in the intestine of carnivorous fish: A case of Monopterus albus Aquaculture Reports Freshwater fish SCFA Microflora Omics |
| title | Study on efficient metabolism mechanism of starch in the intestine of carnivorous fish: A case of Monopterus albus |
| title_full | Study on efficient metabolism mechanism of starch in the intestine of carnivorous fish: A case of Monopterus albus |
| title_fullStr | Study on efficient metabolism mechanism of starch in the intestine of carnivorous fish: A case of Monopterus albus |
| title_full_unstemmed | Study on efficient metabolism mechanism of starch in the intestine of carnivorous fish: A case of Monopterus albus |
| title_short | Study on efficient metabolism mechanism of starch in the intestine of carnivorous fish: A case of Monopterus albus |
| title_sort | study on efficient metabolism mechanism of starch in the intestine of carnivorous fish a case of monopterus albus |
| topic | Freshwater fish SCFA Microflora Omics |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423003344 |
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