<i>OsARF11</i> Promotes Growth, Meristem, Seed, and Vein Formation during Rice Plant Development
The plant hormone auxin acts as a mediator providing positional instructions in a range of developmental processes. Studies in <i>Arabidopsis thaliana</i> L. show that auxin acts in large part via activation of Auxin Response Factors (ARFs) that in turn regulate the expression of downstr...
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MDPI AG
2021-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/8/4089 |
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| author | Katherine Sims Fatemeh Abedi-Samakush Nicole Szulc Monika Gyongyi Macias Honti Jim Mattsson |
| author_facet | Katherine Sims Fatemeh Abedi-Samakush Nicole Szulc Monika Gyongyi Macias Honti Jim Mattsson |
| author_sort | Katherine Sims |
| collection | DOAJ |
| description | The plant hormone auxin acts as a mediator providing positional instructions in a range of developmental processes. Studies in <i>Arabidopsis thaliana</i> L. show that auxin acts in large part via activation of Auxin Response Factors (ARFs) that in turn regulate the expression of downstream genes. The rice (<i>Oryza sativa</i> L.) gene <i>OsARF11</i> is of interest because of its expression in developing rice organs and its high sequence similarity with <i>MONOPTEROS/ARF5</i>, a gene with prominent roles in <i>A. thaliana</i> development. We have assessed the phenotype of homozygous insertion mutants in the <i>OsARF11</i> gene and found that in relation to wildtype, <i>osarf11</i> seedlings produced fewer and shorter roots as well as shorter and less wide leaves. Leaves developed fewer veins and larger areoles. Mature <i>osarf11</i> plants had a reduced root system, fewer branches per panicle, fewer grains per panicle and fewer filled seeds. Mutants had a reduced sensitivity to auxin-mediated callus formation and inhibition of root elongation, and phenylboronic acid (PBA)-mediated inhibition of vein formation. Taken together, our results implicate <i>OsARF11</i> in auxin-mediated growth of multiple organs and leaf veins. <i>OsARF11</i> also appears to play a central role in the formation of lateral root, panicle branch, and grain meristems. |
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| last_indexed | 2024-03-10T12:18:41Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-bc89d64919c14522a7c9bf4072b54f6c2023-11-21T15:41:56ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-04-01228408910.3390/ijms22084089<i>OsARF11</i> Promotes Growth, Meristem, Seed, and Vein Formation during Rice Plant DevelopmentKatherine Sims0Fatemeh Abedi-Samakush1Nicole Szulc2Monika Gyongyi Macias Honti3Jim Mattsson4Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC V5A 1S6, CanadaDepartment of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC V5A 1S6, CanadaDepartment of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC V5A 1S6, CanadaDepartment of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC V5A 1S6, CanadaDepartment of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC V5A 1S6, CanadaThe plant hormone auxin acts as a mediator providing positional instructions in a range of developmental processes. Studies in <i>Arabidopsis thaliana</i> L. show that auxin acts in large part via activation of Auxin Response Factors (ARFs) that in turn regulate the expression of downstream genes. The rice (<i>Oryza sativa</i> L.) gene <i>OsARF11</i> is of interest because of its expression in developing rice organs and its high sequence similarity with <i>MONOPTEROS/ARF5</i>, a gene with prominent roles in <i>A. thaliana</i> development. We have assessed the phenotype of homozygous insertion mutants in the <i>OsARF11</i> gene and found that in relation to wildtype, <i>osarf11</i> seedlings produced fewer and shorter roots as well as shorter and less wide leaves. Leaves developed fewer veins and larger areoles. Mature <i>osarf11</i> plants had a reduced root system, fewer branches per panicle, fewer grains per panicle and fewer filled seeds. Mutants had a reduced sensitivity to auxin-mediated callus formation and inhibition of root elongation, and phenylboronic acid (PBA)-mediated inhibition of vein formation. Taken together, our results implicate <i>OsARF11</i> in auxin-mediated growth of multiple organs and leaf veins. <i>OsARF11</i> also appears to play a central role in the formation of lateral root, panicle branch, and grain meristems.https://www.mdpi.com/1422-0067/22/8/4089auxin response factorauxin signalingauxin perceptionmeristemvein patterningleaf expansion |
| spellingShingle | Katherine Sims Fatemeh Abedi-Samakush Nicole Szulc Monika Gyongyi Macias Honti Jim Mattsson <i>OsARF11</i> Promotes Growth, Meristem, Seed, and Vein Formation during Rice Plant Development International Journal of Molecular Sciences auxin response factor auxin signaling auxin perception meristem vein patterning leaf expansion |
| title | <i>OsARF11</i> Promotes Growth, Meristem, Seed, and Vein Formation during Rice Plant Development |
| title_full | <i>OsARF11</i> Promotes Growth, Meristem, Seed, and Vein Formation during Rice Plant Development |
| title_fullStr | <i>OsARF11</i> Promotes Growth, Meristem, Seed, and Vein Formation during Rice Plant Development |
| title_full_unstemmed | <i>OsARF11</i> Promotes Growth, Meristem, Seed, and Vein Formation during Rice Plant Development |
| title_short | <i>OsARF11</i> Promotes Growth, Meristem, Seed, and Vein Formation during Rice Plant Development |
| title_sort | i osarf11 i promotes growth meristem seed and vein formation during rice plant development |
| topic | auxin response factor auxin signaling auxin perception meristem vein patterning leaf expansion |
| url | https://www.mdpi.com/1422-0067/22/8/4089 |
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