Comparison of Various Soybean Allergen Levels in Genetically and Non-Genetically Modified Soybeans
Several analyses of allergen levels have been reported as part of the safety assessment of genetically modified (GM) soybean; however, few comprehensive analyses have included new allergens. Thus, in this study the levels of eight major soybean allergens, including Gly m 7 (a newly reported soybean...
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MDPI AG
2020-04-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/4/522 |
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| author | Ayato Matsuo Kaho Matsushita Ayano Fukuzumi Naoki Tokumasu Erika Yano Nobuhiro Zaima Tatsuya Moriyama |
| author_facet | Ayato Matsuo Kaho Matsushita Ayano Fukuzumi Naoki Tokumasu Erika Yano Nobuhiro Zaima Tatsuya Moriyama |
| author_sort | Ayato Matsuo |
| collection | DOAJ |
| description | Several analyses of allergen levels have been reported as part of the safety assessment of genetically modified (GM) soybean; however, few comprehensive analyses have included new allergens. Thus, in this study the levels of eight major soybean allergens, including Gly m 7 (a newly reported soybean allergen), were semi-quantitatively detected in six GM soybeans and six non-GM soybeans using antigen-immobilized ELISA and immunoblotting. We also analyzed the IgE-reactivity to these soybeans through immunoblotting, using sera from three soybean-allergic patients. The results showed that there were no significant differences in the levels of the major soybean allergens in the GM and non-GM soybeans. Moreover, there were no significant differences in the serum IgE-reactive protein profiles of the patients, as analyzed using immunoblotting. These results indicate that, in general, CP4-EPSPS-transfected GM soybeans are not more allergenic than non-GM soybeans. |
| first_indexed | 2024-03-10T20:19:34Z |
| format | Article |
| id | doaj.art-bc8b0ab0ee2546ec94d1b94d6a092a52 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T20:19:34Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-bc8b0ab0ee2546ec94d1b94d6a092a522023-11-19T22:17:25ZengMDPI AGFoods2304-81582020-04-019452210.3390/foods9040522Comparison of Various Soybean Allergen Levels in Genetically and Non-Genetically Modified SoybeansAyato Matsuo0Kaho Matsushita1Ayano Fukuzumi2Naoki Tokumasu3Erika Yano4Nobuhiro Zaima5Tatsuya Moriyama6Department of Applied Biological Chemistry, Graduate School of Agriculture, Kindai University, Nara 631-8505, JapanDepartment of Applied Biological Chemistry, Graduate School of Agriculture, Kindai University, Nara 631-8505, JapanDepartment of Applied Biological Chemistry, Graduate School of Agriculture, Kindai University, Nara 631-8505, JapanDepartment of Applied Biological Chemistry, Graduate School of Agriculture, Kindai University, Nara 631-8505, JapanDepartment of Applied Biological Chemistry, Graduate School of Agriculture, Kindai University, Nara 631-8505, JapanDepartment of Applied Biological Chemistry, Graduate School of Agriculture, Kindai University, Nara 631-8505, JapanDepartment of Applied Biological Chemistry, Graduate School of Agriculture, Kindai University, Nara 631-8505, JapanSeveral analyses of allergen levels have been reported as part of the safety assessment of genetically modified (GM) soybean; however, few comprehensive analyses have included new allergens. Thus, in this study the levels of eight major soybean allergens, including Gly m 7 (a newly reported soybean allergen), were semi-quantitatively detected in six GM soybeans and six non-GM soybeans using antigen-immobilized ELISA and immunoblotting. We also analyzed the IgE-reactivity to these soybeans through immunoblotting, using sera from three soybean-allergic patients. The results showed that there were no significant differences in the levels of the major soybean allergens in the GM and non-GM soybeans. Moreover, there were no significant differences in the serum IgE-reactive protein profiles of the patients, as analyzed using immunoblotting. These results indicate that, in general, CP4-EPSPS-transfected GM soybeans are not more allergenic than non-GM soybeans.https://www.mdpi.com/2304-8158/9/4/522soybeanallergensallergenicitygenetically modifiedGly m 7 |
| spellingShingle | Ayato Matsuo Kaho Matsushita Ayano Fukuzumi Naoki Tokumasu Erika Yano Nobuhiro Zaima Tatsuya Moriyama Comparison of Various Soybean Allergen Levels in Genetically and Non-Genetically Modified Soybeans Foods soybean allergens allergenicity genetically modified Gly m 7 |
| title | Comparison of Various Soybean Allergen Levels in Genetically and Non-Genetically Modified Soybeans |
| title_full | Comparison of Various Soybean Allergen Levels in Genetically and Non-Genetically Modified Soybeans |
| title_fullStr | Comparison of Various Soybean Allergen Levels in Genetically and Non-Genetically Modified Soybeans |
| title_full_unstemmed | Comparison of Various Soybean Allergen Levels in Genetically and Non-Genetically Modified Soybeans |
| title_short | Comparison of Various Soybean Allergen Levels in Genetically and Non-Genetically Modified Soybeans |
| title_sort | comparison of various soybean allergen levels in genetically and non genetically modified soybeans |
| topic | soybean allergens allergenicity genetically modified Gly m 7 |
| url | https://www.mdpi.com/2304-8158/9/4/522 |
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