Effect of earthworm and papain enzyme concentration on antioxidant activity of earthworm (Eudrilus eugeniae) protein hydrolysate
The earthworm Eudrilus eugeniae (African Night Crawler) can be used as a raw material for making protein hydrolysates. Hydrolysis of earthworms using papain enzyme produces peptides that have bioactivity as antioxidants. Antioxidant activity in earthworm hydrolysate is caused by phenolic compounds t...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Brawijaya, Fakultas Teknologi Pertanian
2023-12-01
|
| Series: | Advances in Food Science, Sustainable Agriculture, and Agroindustrial Engineering |
| Subjects: | |
| Online Access: | https://afssaae.ub.ac.id/index.php/afssaae/article/view/15608/220 |
| _version_ | 1827390220404785152 |
|---|---|
| author | Dianah Salsabila Nur Hidayat Suprayogi |
| author_facet | Dianah Salsabila Nur Hidayat Suprayogi |
| author_sort | Dianah Salsabila |
| collection | DOAJ |
| description | The earthworm Eudrilus eugeniae (African Night Crawler) can be used as a raw material for making protein hydrolysates. Hydrolysis of earthworms using papain enzyme produces peptides that have bioactivity as antioxidants. Antioxidant activity in earthworm hydrolysate is caused by phenolic compounds that have an important role in neutralizing free radicals. This study aims to determine the effect of earthworm concentration and papain enzyme concentration on soluble protein, total phenolic content, and antioxidant activity. This study used the Randomized Block Design (RBD) method arranged with two factors, namely earthworm concentration (20% 30%, and 40% (w/v)) and papain enzyme concentration (8%, 10%, and 12% (w/v)) with three replications. Data analysis used Analysis of Variance (ANOVA) and continued with the DMRT test. The results showed that earthworm concentration, papain enzyme concentration, and the interaction between the two factors had a significant effect (p<0.05) on the test results of soluble protein, total phenolic content, and antioxidant activity. The treatment with earthworm concentration (40% (w/v)) and papain enzyme concentration (12% (w/v)) produced the highest mean soluble protein, total phenolic content, and antioxidant activity of 11.47%, 23.07 mg GAE/mL hydrolysate, and 3301.42 ppm. |
| first_indexed | 2024-03-08T16:48:20Z |
| format | Article |
| id | doaj.art-bc8ba00813ba4c68a8b7f23cb59c2db4 |
| institution | Directory Open Access Journal |
| issn | 2622-5921 |
| language | English |
| last_indexed | 2024-03-08T16:48:20Z |
| publishDate | 2023-12-01 |
| publisher | Universitas Brawijaya, Fakultas Teknologi Pertanian |
| record_format | Article |
| series | Advances in Food Science, Sustainable Agriculture, and Agroindustrial Engineering |
| spelling | doaj.art-bc8ba00813ba4c68a8b7f23cb59c2db42024-01-05T06:55:27ZengUniversitas Brawijaya, Fakultas Teknologi PertanianAdvances in Food Science, Sustainable Agriculture, and Agroindustrial Engineering2622-59212023-12-0164362373 10.21776/ub.afssaae.2023.006.04.4Effect of earthworm and papain enzyme concentration on antioxidant activity of earthworm (Eudrilus eugeniae) protein hydrolysateDianah Salsabila0Nur Hidayat1Suprayogi2Universitas BrawijayaUniversitas BrawijayaUniversitas BrawijayaThe earthworm Eudrilus eugeniae (African Night Crawler) can be used as a raw material for making protein hydrolysates. Hydrolysis of earthworms using papain enzyme produces peptides that have bioactivity as antioxidants. Antioxidant activity in earthworm hydrolysate is caused by phenolic compounds that have an important role in neutralizing free radicals. This study aims to determine the effect of earthworm concentration and papain enzyme concentration on soluble protein, total phenolic content, and antioxidant activity. This study used the Randomized Block Design (RBD) method arranged with two factors, namely earthworm concentration (20% 30%, and 40% (w/v)) and papain enzyme concentration (8%, 10%, and 12% (w/v)) with three replications. Data analysis used Analysis of Variance (ANOVA) and continued with the DMRT test. The results showed that earthworm concentration, papain enzyme concentration, and the interaction between the two factors had a significant effect (p<0.05) on the test results of soluble protein, total phenolic content, and antioxidant activity. The treatment with earthworm concentration (40% (w/v)) and papain enzyme concentration (12% (w/v)) produced the highest mean soluble protein, total phenolic content, and antioxidant activity of 11.47%, 23.07 mg GAE/mL hydrolysate, and 3301.42 ppm.https://afssaae.ub.ac.id/index.php/afssaae/article/view/15608/220antioxidant activityearthworm eudrilus eugeniaepapain enzymesoluble proteintotal phenolic content |
| spellingShingle | Dianah Salsabila Nur Hidayat Suprayogi Effect of earthworm and papain enzyme concentration on antioxidant activity of earthworm (Eudrilus eugeniae) protein hydrolysate Advances in Food Science, Sustainable Agriculture, and Agroindustrial Engineering antioxidant activity earthworm eudrilus eugeniae papain enzyme soluble protein total phenolic content |
| title | Effect of earthworm and papain enzyme concentration on antioxidant activity of earthworm (Eudrilus eugeniae) protein hydrolysate |
| title_full | Effect of earthworm and papain enzyme concentration on antioxidant activity of earthworm (Eudrilus eugeniae) protein hydrolysate |
| title_fullStr | Effect of earthworm and papain enzyme concentration on antioxidant activity of earthworm (Eudrilus eugeniae) protein hydrolysate |
| title_full_unstemmed | Effect of earthworm and papain enzyme concentration on antioxidant activity of earthworm (Eudrilus eugeniae) protein hydrolysate |
| title_short | Effect of earthworm and papain enzyme concentration on antioxidant activity of earthworm (Eudrilus eugeniae) protein hydrolysate |
| title_sort | effect of earthworm and papain enzyme concentration on antioxidant activity of earthworm eudrilus eugeniae protein hydrolysate |
| topic | antioxidant activity earthworm eudrilus eugeniae papain enzyme soluble protein total phenolic content |
| url | https://afssaae.ub.ac.id/index.php/afssaae/article/view/15608/220 |
| work_keys_str_mv | AT dianahsalsabila effectofearthwormandpapainenzymeconcentrationonantioxidantactivityofearthwormeudriluseugeniaeproteinhydrolysate AT nurhidayat effectofearthwormandpapainenzymeconcentrationonantioxidantactivityofearthwormeudriluseugeniaeproteinhydrolysate AT suprayogi effectofearthwormandpapainenzymeconcentrationonantioxidantactivityofearthwormeudriluseugeniaeproteinhydrolysate |