Use of AFLP marker system on sugarcane somaclones to study their resistance to rust
AFLPs (amplified fragment length polymorphism) was carried out from genomic DNA of five rust resistant sugar cane somaclons and their susceptible donor, Saccharum officinarum var B 4362, using three combinations of primers (EcoRI/aca: MseI/acc; EcoRI/aca: MseI/atg and EcoRI/aca: MseI/agg). Six polym...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidad Central Marta Abreu de Las Villas
2002-10-01
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| Series: | Biotecnología Vegetal |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/194 |
| _version_ | 1818598158276493312 |
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| author | María Ileana Oloriz Luis Rojas Víctor Gil Milady Mendoza Ariel Arencibia Elva Rosa Carmona Elio Jiménez |
| author_facet | María Ileana Oloriz Luis Rojas Víctor Gil Milady Mendoza Ariel Arencibia Elva Rosa Carmona Elio Jiménez |
| author_sort | María Ileana Oloriz |
| collection | DOAJ |
| description | AFLPs (amplified fragment length polymorphism) was carried out from genomic DNA of five rust resistant sugar cane somaclons and their susceptible donor, Saccharum officinarum var B 4362, using three combinations of primers (EcoRI/aca: MseI/acc; EcoRI/aca: MseI/atg and EcoRI/aca: MseI/agg). Six polymorphic bands were obtained, two of these only appeared in the resistant genotypes, which are probably DNA sequences, related to rust resistance locus. These fragments have been cloned to study their nucleotide sequence and to investigate their roll in resistance mechanism develop by these mutants during Puccinia melanocephala infection.
Key words: molecular markers, Puccinia. melanocephala, Saccharum |
| first_indexed | 2024-12-16T11:59:15Z |
| format | Article |
| id | doaj.art-bc8c67a37471471b9c29ae7e23fb5dbd |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-12-16T11:59:15Z |
| publishDate | 2002-10-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-bc8c67a37471471b9c29ae7e23fb5dbd2022-12-21T22:32:29ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472002-10-0124170Use of AFLP marker system on sugarcane somaclones to study their resistance to rustMaría Ileana OlorizLuis RojasVíctor GilMilady MendozaAriel ArencibiaElva Rosa CarmonaElio JiménezAFLPs (amplified fragment length polymorphism) was carried out from genomic DNA of five rust resistant sugar cane somaclons and their susceptible donor, Saccharum officinarum var B 4362, using three combinations of primers (EcoRI/aca: MseI/acc; EcoRI/aca: MseI/atg and EcoRI/aca: MseI/agg). Six polymorphic bands were obtained, two of these only appeared in the resistant genotypes, which are probably DNA sequences, related to rust resistance locus. These fragments have been cloned to study their nucleotide sequence and to investigate their roll in resistance mechanism develop by these mutants during Puccinia melanocephala infection. Key words: molecular markers, Puccinia. melanocephala, Saccharumhttps://revista.ibp.co.cu/index.php/BV/article/view/194 |
| spellingShingle | María Ileana Oloriz Luis Rojas Víctor Gil Milady Mendoza Ariel Arencibia Elva Rosa Carmona Elio Jiménez Use of AFLP marker system on sugarcane somaclones to study their resistance to rust Biotecnología Vegetal |
| title | Use of AFLP marker system on sugarcane somaclones to study their resistance to rust |
| title_full | Use of AFLP marker system on sugarcane somaclones to study their resistance to rust |
| title_fullStr | Use of AFLP marker system on sugarcane somaclones to study their resistance to rust |
| title_full_unstemmed | Use of AFLP marker system on sugarcane somaclones to study their resistance to rust |
| title_short | Use of AFLP marker system on sugarcane somaclones to study their resistance to rust |
| title_sort | use of aflp marker system on sugarcane somaclones to study their resistance to rust |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/194 |
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