New Cell Lines Derived from Laboratory Colony <i>Triatoma infestans</i> and <i>Rhodnius prolixus</i>, Vectors of <i>Trypanosoma cruzi</i>, Do Not Harbour Triatoma Virus
Triatomine bugs of the genera <i>Triatoma</i> and <i>Rhodnius</i> are vectors of Chagas disease, a neglected tropical disease of humans in South America caused by <i>Trypanosoma cruzi</i>. Triatoma virus (TrV), a natural pathogen of <i>Triatoma infestans<...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/13/10/906 |
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| author | Rebekah Penrice-Randal Catherine Hartley Alexandra Beliavskaia Xiaofeng Dong Luke Brandner-Garrod Miranda Whitten Lesley Bell-Sakyi |
| author_facet | Rebekah Penrice-Randal Catherine Hartley Alexandra Beliavskaia Xiaofeng Dong Luke Brandner-Garrod Miranda Whitten Lesley Bell-Sakyi |
| author_sort | Rebekah Penrice-Randal |
| collection | DOAJ |
| description | Triatomine bugs of the genera <i>Triatoma</i> and <i>Rhodnius</i> are vectors of Chagas disease, a neglected tropical disease of humans in South America caused by <i>Trypanosoma cruzi</i>. Triatoma virus (TrV), a natural pathogen of <i>Triatoma infestans</i>, has been proposed as a possible tool for the bio-control of triatomine bugs, but research into this virus has been hampered by a lack of suitable host cells for <i>in vitro</i> propagation. Here we report establishment and partial characterisation of continuous cell lines from embryos of <i>T. infestans</i> (TIE/LULS54) and <i>Rhodnius prolixus</i> (RPE/LULS53 and RPE/LULS57). RNAseq screening by a sequence-independent, single primer amplification approach confirmed the absence of TrV and other RNA viruses known to infect <i>R. prolixus</i>, indicating that these new cell lines could be used for propagation of TrV. |
| first_indexed | 2024-03-09T20:04:08Z |
| format | Article |
| id | doaj.art-bc8cdd3675234a178d2cd117c482f965 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T20:04:08Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-bc8cdd3675234a178d2cd117c482f9652023-11-24T00:38:02ZengMDPI AGInsects2075-44502022-10-01131090610.3390/insects13100906New Cell Lines Derived from Laboratory Colony <i>Triatoma infestans</i> and <i>Rhodnius prolixus</i>, Vectors of <i>Trypanosoma cruzi</i>, Do Not Harbour Triatoma VirusRebekah Penrice-Randal0Catherine Hartley1Alexandra Beliavskaia2Xiaofeng Dong3Luke Brandner-Garrod4Miranda Whitten5Lesley Bell-Sakyi6Department of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, 146 Brownlow Hill, Liverpool L3 5RF, UKDepartment of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, 146 Brownlow Hill, Liverpool L3 5RF, UKDepartment of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, 146 Brownlow Hill, Liverpool L3 5RF, UKDepartment of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, 146 Brownlow Hill, Liverpool L3 5RF, UKDepartment of Infection Biology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UKSwansea University Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UKDepartment of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, 146 Brownlow Hill, Liverpool L3 5RF, UKTriatomine bugs of the genera <i>Triatoma</i> and <i>Rhodnius</i> are vectors of Chagas disease, a neglected tropical disease of humans in South America caused by <i>Trypanosoma cruzi</i>. Triatoma virus (TrV), a natural pathogen of <i>Triatoma infestans</i>, has been proposed as a possible tool for the bio-control of triatomine bugs, but research into this virus has been hampered by a lack of suitable host cells for <i>in vitro</i> propagation. Here we report establishment and partial characterisation of continuous cell lines from embryos of <i>T. infestans</i> (TIE/LULS54) and <i>Rhodnius prolixus</i> (RPE/LULS53 and RPE/LULS57). RNAseq screening by a sequence-independent, single primer amplification approach confirmed the absence of TrV and other RNA viruses known to infect <i>R. prolixus</i>, indicating that these new cell lines could be used for propagation of TrV.https://www.mdpi.com/2075-4450/13/10/906<i>Triatoma infestans</i><i>Rhodnius prolixus</i>insectvectorkissing bugcell line |
| spellingShingle | Rebekah Penrice-Randal Catherine Hartley Alexandra Beliavskaia Xiaofeng Dong Luke Brandner-Garrod Miranda Whitten Lesley Bell-Sakyi New Cell Lines Derived from Laboratory Colony <i>Triatoma infestans</i> and <i>Rhodnius prolixus</i>, Vectors of <i>Trypanosoma cruzi</i>, Do Not Harbour Triatoma Virus Insects <i>Triatoma infestans</i> <i>Rhodnius prolixus</i> insect vector kissing bug cell line |
| title | New Cell Lines Derived from Laboratory Colony <i>Triatoma infestans</i> and <i>Rhodnius prolixus</i>, Vectors of <i>Trypanosoma cruzi</i>, Do Not Harbour Triatoma Virus |
| title_full | New Cell Lines Derived from Laboratory Colony <i>Triatoma infestans</i> and <i>Rhodnius prolixus</i>, Vectors of <i>Trypanosoma cruzi</i>, Do Not Harbour Triatoma Virus |
| title_fullStr | New Cell Lines Derived from Laboratory Colony <i>Triatoma infestans</i> and <i>Rhodnius prolixus</i>, Vectors of <i>Trypanosoma cruzi</i>, Do Not Harbour Triatoma Virus |
| title_full_unstemmed | New Cell Lines Derived from Laboratory Colony <i>Triatoma infestans</i> and <i>Rhodnius prolixus</i>, Vectors of <i>Trypanosoma cruzi</i>, Do Not Harbour Triatoma Virus |
| title_short | New Cell Lines Derived from Laboratory Colony <i>Triatoma infestans</i> and <i>Rhodnius prolixus</i>, Vectors of <i>Trypanosoma cruzi</i>, Do Not Harbour Triatoma Virus |
| title_sort | new cell lines derived from laboratory colony i triatoma infestans i and i rhodnius prolixus i vectors of i trypanosoma cruzi i do not harbour triatoma virus |
| topic | <i>Triatoma infestans</i> <i>Rhodnius prolixus</i> insect vector kissing bug cell line |
| url | https://www.mdpi.com/2075-4450/13/10/906 |
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