Serum Nitric Oxide and Plasma HbA1c Levels in Type 2 Diabetes Mellitus Patients
Introduction: Diabetes Mellitus (DM) is a metabolic disorder characterised by hyperglycaemia and insufficiency of secretion or action of endogenous insulin. Glycated haemoglobin (HbA1c) is commonly used as reliable index to determine cumulative glycaemic history of preceding 2-3 months. Nitric Oxide...
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| Format: | Article |
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JCDR Research and Publications Private Limited
2019-09-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/13139/41952_CE[Ra1]_F(KM)_PF1(TR_SHU)_PN(SL).pdf |
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| author | Shah Yasir Yousuf Sudha Ambiger Dnyanesh Morkar |
| author_facet | Shah Yasir Yousuf Sudha Ambiger Dnyanesh Morkar |
| author_sort | Shah Yasir Yousuf |
| collection | DOAJ |
| description | Introduction: Diabetes Mellitus (DM) is a metabolic disorder characterised by hyperglycaemia and insufficiency of secretion or action of endogenous insulin. Glycated haemoglobin (HbA1c) is commonly used as reliable index to determine cumulative glycaemic history of preceding 2-3 months. Nitric Oxide (NO) is a potent vasodilator and an endothelium-relaxing factor. Low serum Nitric Oxide (NO) levels are known to be associated with endothelial dysfunction in Type 2 Diabetes Mellitus (T2DM). Aim: To estimate the serum nitric oxide levels in type 2 diabetic patients and correlate it with serum HbA1c levels. Materials and Methods: The cross-sectional study comprised of 100 (50 male and 50 female) T2DM patients who attended KLE’S Dr. Prabhakar Kore Hospital and Medical Research Centre, Belagavi, Karnataka, India. Fasting Plasma Glucose (FPG) was measured by Hexokinase method using Roche/Hitachi COBAS 6000 Fully Automated Analyser. HbA1c was estimated using a Bio-Rad D-10 HbA1c program and serum NO was estimated by Classical Griess Reaction. Data was analysed using independent t-test within the groups and Karl Pearson Correlation Coefficient in between the groups. The p<0.05 was considered as statistically significant. Results: Statistically significant positive correlation was observed between NO and with HbA1c (r=0.7674) and NO with FBS (r=0.5688). Conclusion: Hyperglycaemia increases NO levels in T2DM patients. Measurement of nitric oxide in early course of diabetes may help in preventing the progression and development of complications like endothelial dysfunction. |
| first_indexed | 2024-12-11T04:07:50Z |
| format | Article |
| id | doaj.art-bc8e17915fd745c4a5fa914bde29d570 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-11T04:07:50Z |
| publishDate | 2019-09-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-bc8e17915fd745c4a5fa914bde29d5702022-12-22T01:21:29ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2019-09-01139BC04BC0610.7860/JCDR/2019/41952.13139Serum Nitric Oxide and Plasma HbA1c Levels in Type 2 Diabetes Mellitus PatientsShah Yasir Yousuf0Sudha Ambiger1Dnyanesh Morkar2Student, Department of Biochemistry, Jawaharlal Nehru Medical College, Belagavi, Karnataka, India.Assistant Professor, Department of Biochemistry, Jawaharlal Nehru Medical College, Belagavi, Karnataka, India.Associate Professor, Department of General Medicine, Jawaharlal Nehru Medical College, Belagavi, Karnataka, India.Introduction: Diabetes Mellitus (DM) is a metabolic disorder characterised by hyperglycaemia and insufficiency of secretion or action of endogenous insulin. Glycated haemoglobin (HbA1c) is commonly used as reliable index to determine cumulative glycaemic history of preceding 2-3 months. Nitric Oxide (NO) is a potent vasodilator and an endothelium-relaxing factor. Low serum Nitric Oxide (NO) levels are known to be associated with endothelial dysfunction in Type 2 Diabetes Mellitus (T2DM). Aim: To estimate the serum nitric oxide levels in type 2 diabetic patients and correlate it with serum HbA1c levels. Materials and Methods: The cross-sectional study comprised of 100 (50 male and 50 female) T2DM patients who attended KLE’S Dr. Prabhakar Kore Hospital and Medical Research Centre, Belagavi, Karnataka, India. Fasting Plasma Glucose (FPG) was measured by Hexokinase method using Roche/Hitachi COBAS 6000 Fully Automated Analyser. HbA1c was estimated using a Bio-Rad D-10 HbA1c program and serum NO was estimated by Classical Griess Reaction. Data was analysed using independent t-test within the groups and Karl Pearson Correlation Coefficient in between the groups. The p<0.05 was considered as statistically significant. Results: Statistically significant positive correlation was observed between NO and with HbA1c (r=0.7674) and NO with FBS (r=0.5688). Conclusion: Hyperglycaemia increases NO levels in T2DM patients. Measurement of nitric oxide in early course of diabetes may help in preventing the progression and development of complications like endothelial dysfunction.https://jcdr.net/articles/PDF/13139/41952_CE[Ra1]_F(KM)_PF1(TR_SHU)_PN(SL).pdfdiabetic complicationsendothelial dysfunctionglycated haemoglobingriess reaction |
| spellingShingle | Shah Yasir Yousuf Sudha Ambiger Dnyanesh Morkar Serum Nitric Oxide and Plasma HbA1c Levels in Type 2 Diabetes Mellitus Patients Journal of Clinical and Diagnostic Research diabetic complications endothelial dysfunction glycated haemoglobin griess reaction |
| title | Serum Nitric Oxide and Plasma HbA1c Levels in Type 2 Diabetes Mellitus Patients |
| title_full | Serum Nitric Oxide and Plasma HbA1c Levels in Type 2 Diabetes Mellitus Patients |
| title_fullStr | Serum Nitric Oxide and Plasma HbA1c Levels in Type 2 Diabetes Mellitus Patients |
| title_full_unstemmed | Serum Nitric Oxide and Plasma HbA1c Levels in Type 2 Diabetes Mellitus Patients |
| title_short | Serum Nitric Oxide and Plasma HbA1c Levels in Type 2 Diabetes Mellitus Patients |
| title_sort | serum nitric oxide and plasma hba1c levels in type 2 diabetes mellitus patients |
| topic | diabetic complications endothelial dysfunction glycated haemoglobin griess reaction |
| url | https://jcdr.net/articles/PDF/13139/41952_CE[Ra1]_F(KM)_PF1(TR_SHU)_PN(SL).pdf |
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