Antagonistic interaction between two key endodontic pathogens Enterococcus faecalis and Fusobacterium nucleatum
ABSTRACTBackground Endodontic infections are known to be caused by pathogenic bacteria. Numerous previous studies found that both Fusobacterium nucleatum and Enterococcus faecalis are associated with endodontic infections, with Fusobacterium nucleatum more abundant in primary infection while Enteroc...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Journal of Oral Microbiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20002297.2022.2149448 |
| _version_ | 1827632219423768576 |
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| author | Doudou Xiang Pu-Ting Dong Lujia Cen Batbileg Bor Renate Lux Wenyuan Shi Qing Yu Xuesong He Tingxi Wu |
| author_facet | Doudou Xiang Pu-Ting Dong Lujia Cen Batbileg Bor Renate Lux Wenyuan Shi Qing Yu Xuesong He Tingxi Wu |
| author_sort | Doudou Xiang |
| collection | DOAJ |
| description | ABSTRACTBackground Endodontic infections are known to be caused by pathogenic bacteria. Numerous previous studies found that both Fusobacterium nucleatum and Enterococcus faecalis are associated with endodontic infections, with Fusobacterium nucleatum more abundant in primary infection while Enterococcus faecalis more abundant in secondary infection. Little is known about the potential interactions between different endodontic pathogens.Objective This study aims to investigate the potential interaction between F. nucleatum and E. faecalis via phenotypical and genetic approaches.Methods Physical and physiological interactions of F. nucleatum and E. faecalis under both planktonic and biofilm conditions were measured with co-aggregation and competition assays. The mechanisms behind these interactions were revealed with genetic screening and biochemical measurements.Results E. faecalis was found to physically bind to F. nucleatum under both in vitro planktonic and biofilm conditions, and this interaction requires F. nucleatum fap2, a galactose-inhibitable adhesin-encoding gene. Under our experimental conditions, E. faecalis exhibits a strong killing ability against F. nucleatum by generating an acidic micro-environment and producing hydrogen peroxide (H2O2). Finally, the binding and killing capacities of E. faecalis were found to be necessary to invade and dominate a pre-established in vitro F. nucleatum biofilm.Conclusions This study reveals multifaceted mechanisms underlying the physical binding and antagonistic interaction between F. nucleatum and E. faecalis, which could play a potential role in the shift of microbial composition in primary and secondary endodontic infections. |
| first_indexed | 2024-03-09T14:32:58Z |
| format | Article |
| id | doaj.art-bc8eb3ac8f1946beaa28ee42ae3b3b59 |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-03-09T14:32:58Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-bc8eb3ac8f1946beaa28ee42ae3b3b592023-11-27T17:25:00ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972023-12-0115110.1080/20002297.2022.2149448Antagonistic interaction between two key endodontic pathogens Enterococcus faecalis and Fusobacterium nucleatumDoudou Xiang0Pu-Ting Dong1Lujia Cen2Batbileg Bor3Renate Lux4Wenyuan Shi5Qing Yu6Xuesong He7Tingxi Wu8Department of Operative Dentistry and Endodontics, School of Stomatology, The Fourth Military Medical University, Xi’an, Shaanxi, ChinaDepartment of Microbiology, The Forsyth Institute, Cambridge, Massachusetts, USADepartment of Microbiology, The Forsyth Institute, Cambridge, Massachusetts, USADepartment of Microbiology, The Forsyth Institute, Cambridge, Massachusetts, USASchool of Dentistry, UCLA, Los Angeles, California, USADepartment of Microbiology, The Forsyth Institute, Cambridge, Massachusetts, USADepartment of Operative Dentistry and Endodontics, School of Stomatology, The Fourth Military Medical University, Xi’an, Shaanxi, ChinaDepartment of Microbiology, The Forsyth Institute, Cambridge, Massachusetts, USADepartment of Microbiology, The Forsyth Institute, Cambridge, Massachusetts, USAABSTRACTBackground Endodontic infections are known to be caused by pathogenic bacteria. Numerous previous studies found that both Fusobacterium nucleatum and Enterococcus faecalis are associated with endodontic infections, with Fusobacterium nucleatum more abundant in primary infection while Enterococcus faecalis more abundant in secondary infection. Little is known about the potential interactions between different endodontic pathogens.Objective This study aims to investigate the potential interaction between F. nucleatum and E. faecalis via phenotypical and genetic approaches.Methods Physical and physiological interactions of F. nucleatum and E. faecalis under both planktonic and biofilm conditions were measured with co-aggregation and competition assays. The mechanisms behind these interactions were revealed with genetic screening and biochemical measurements.Results E. faecalis was found to physically bind to F. nucleatum under both in vitro planktonic and biofilm conditions, and this interaction requires F. nucleatum fap2, a galactose-inhibitable adhesin-encoding gene. Under our experimental conditions, E. faecalis exhibits a strong killing ability against F. nucleatum by generating an acidic micro-environment and producing hydrogen peroxide (H2O2). Finally, the binding and killing capacities of E. faecalis were found to be necessary to invade and dominate a pre-established in vitro F. nucleatum biofilm.Conclusions This study reveals multifaceted mechanisms underlying the physical binding and antagonistic interaction between F. nucleatum and E. faecalis, which could play a potential role in the shift of microbial composition in primary and secondary endodontic infections.https://www.tandfonline.com/doi/10.1080/20002297.2022.2149448Interspecies interactioncoaggregationEnterococcus faecalisFusobacterium nucleatumfap2H2O2 |
| spellingShingle | Doudou Xiang Pu-Ting Dong Lujia Cen Batbileg Bor Renate Lux Wenyuan Shi Qing Yu Xuesong He Tingxi Wu Antagonistic interaction between two key endodontic pathogens Enterococcus faecalis and Fusobacterium nucleatum Journal of Oral Microbiology Interspecies interaction coaggregation Enterococcus faecalis Fusobacterium nucleatum fap2 H2O2 |
| title | Antagonistic interaction between two key endodontic pathogens Enterococcus faecalis and Fusobacterium nucleatum |
| title_full | Antagonistic interaction between two key endodontic pathogens Enterococcus faecalis and Fusobacterium nucleatum |
| title_fullStr | Antagonistic interaction between two key endodontic pathogens Enterococcus faecalis and Fusobacterium nucleatum |
| title_full_unstemmed | Antagonistic interaction between two key endodontic pathogens Enterococcus faecalis and Fusobacterium nucleatum |
| title_short | Antagonistic interaction between two key endodontic pathogens Enterococcus faecalis and Fusobacterium nucleatum |
| title_sort | antagonistic interaction between two key endodontic pathogens enterococcus faecalis and fusobacterium nucleatum |
| topic | Interspecies interaction coaggregation Enterococcus faecalis Fusobacterium nucleatum fap2 H2O2 |
| url | https://www.tandfonline.com/doi/10.1080/20002297.2022.2149448 |
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