β-Klotho Promotes the Development of Intrauterine Adhesions via the PI3K/AKT Signaling Pathway
Intrauterine adhesion (IUA) refers to injury to the basal layer of the endometrium, which can be caused by various factors. It is often accompanied by clinical symptoms such as abnormal menstruation, infertility, recurrent abortion, and periodic abdominal pain. In recent years, a number of studies h...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/19/11294 |
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| author | Zizhen Guo Yuqing Wang Xiaoyang Wen Xinxin Xu Lei Yan |
| author_facet | Zizhen Guo Yuqing Wang Xiaoyang Wen Xinxin Xu Lei Yan |
| author_sort | Zizhen Guo |
| collection | DOAJ |
| description | Intrauterine adhesion (IUA) refers to injury to the basal layer of the endometrium, which can be caused by various factors. It is often accompanied by clinical symptoms such as abnormal menstruation, infertility, recurrent abortion, and periodic abdominal pain. In recent years, a number of studies have reported the effects of β-Klotho (KLB) on the occurrence and development of human tumors and fibrotic diseases, but its relationship with endometrial fibroblasts and endometrial fibrosis has not been elucidated. In this study, we compared the expression of KLB in endometrial stromal cells (ESCs) from patients with IUA and normal controls. We constructed animal and cell models of IUA and conducted expression verification and functional experiments on KLB. We found that the expression of KLB was significantly increased in the ESCs of IUA patients and rat models compared with the controls. The overexpression of KLB could promote the proliferation and fibrosis of ESCs. In addition, the overexpression of KLB activated the PI3K/AKT signaling pathway in ESCs. Our study shows that KLB protein is highly expressed in the ESCs of patients with IUA and can enhance stromal cell proliferation and cell fibrosis by activating the PI3K/AKT pathway, thus promoting the development of IUA. |
| first_indexed | 2024-03-09T21:41:17Z |
| format | Article |
| id | doaj.art-bc90af44f55244ebb38f4e15b3dd1c03 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:41:17Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-bc90af44f55244ebb38f4e15b3dd1c032023-11-23T20:30:37ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123191129410.3390/ijms231911294β-Klotho Promotes the Development of Intrauterine Adhesions via the PI3K/AKT Signaling PathwayZizhen Guo0Yuqing Wang1Xiaoyang Wen2Xinxin Xu3Lei Yan4Center for Reproductive Medicine, Shandong University, Jinan 250000, ChinaCenter for Reproductive Medicine, Shandong University, Jinan 250000, ChinaCenter for Reproductive Medicine, Shandong University, Jinan 250000, ChinaCenter for Reproductive Medicine, Shandong University, Jinan 250000, ChinaCenter for Reproductive Medicine, Shandong University, Jinan 250000, ChinaIntrauterine adhesion (IUA) refers to injury to the basal layer of the endometrium, which can be caused by various factors. It is often accompanied by clinical symptoms such as abnormal menstruation, infertility, recurrent abortion, and periodic abdominal pain. In recent years, a number of studies have reported the effects of β-Klotho (KLB) on the occurrence and development of human tumors and fibrotic diseases, but its relationship with endometrial fibroblasts and endometrial fibrosis has not been elucidated. In this study, we compared the expression of KLB in endometrial stromal cells (ESCs) from patients with IUA and normal controls. We constructed animal and cell models of IUA and conducted expression verification and functional experiments on KLB. We found that the expression of KLB was significantly increased in the ESCs of IUA patients and rat models compared with the controls. The overexpression of KLB could promote the proliferation and fibrosis of ESCs. In addition, the overexpression of KLB activated the PI3K/AKT signaling pathway in ESCs. Our study shows that KLB protein is highly expressed in the ESCs of patients with IUA and can enhance stromal cell proliferation and cell fibrosis by activating the PI3K/AKT pathway, thus promoting the development of IUA.https://www.mdpi.com/1422-0067/23/19/11294intrauterine adhesionβ-KlothoPI3K/AKTfibrosis |
| spellingShingle | Zizhen Guo Yuqing Wang Xiaoyang Wen Xinxin Xu Lei Yan β-Klotho Promotes the Development of Intrauterine Adhesions via the PI3K/AKT Signaling Pathway International Journal of Molecular Sciences intrauterine adhesion β-Klotho PI3K/AKT fibrosis |
| title | β-Klotho Promotes the Development of Intrauterine Adhesions via the PI3K/AKT Signaling Pathway |
| title_full | β-Klotho Promotes the Development of Intrauterine Adhesions via the PI3K/AKT Signaling Pathway |
| title_fullStr | β-Klotho Promotes the Development of Intrauterine Adhesions via the PI3K/AKT Signaling Pathway |
| title_full_unstemmed | β-Klotho Promotes the Development of Intrauterine Adhesions via the PI3K/AKT Signaling Pathway |
| title_short | β-Klotho Promotes the Development of Intrauterine Adhesions via the PI3K/AKT Signaling Pathway |
| title_sort | β klotho promotes the development of intrauterine adhesions via the pi3k akt signaling pathway |
| topic | intrauterine adhesion β-Klotho PI3K/AKT fibrosis |
| url | https://www.mdpi.com/1422-0067/23/19/11294 |
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