Induction of avirulence by AVR-Pita1 in virulent U.S. field isolates of Magnaporthe oryzae
The AVR-Pita1 gene, from the Chinese isolate O-137 of Magnaporthe oryzae, is an effector that determines the efficacy of the Pi-ta rice blast resistance gene. In the present study, the avirulence function of AVR-Pita1 was induced by transformation of field isolates (TM2, ZN19, B2 and B8) that origin...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2014-02-01
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| Series: | Crop Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514113000536 |
| Summary: | The AVR-Pita1 gene, from the Chinese isolate O-137 of Magnaporthe oryzae, is an effector that determines the efficacy of the Pi-ta rice blast resistance gene. In the present study, the avirulence function of AVR-Pita1 was induced by transformation of field isolates (TM2, ZN19, B2 and B8) that originally were collected from the U.S. and are virulent on Pi-ta-carrying rice cultivars. The presence of AVR-Pita1 from O-137 in independent transformants was detected by PCR using AVR-Pita1 specific primers and verified by DNA sequencing and Southern blot analysis using the AVR-Pita1 coding region as a probe. The results of pathogenicity assays showed that the AVR-Pita1-transformed isolates were not able to infect rice cultivars Katy and Drew carrying Pi-ta. Control isolates that were transformed with inserts lacking the AVR-Pita1 gene remained virulent. Our findings demonstrate that AVR-Pita1 can be used to induce novel gene-specific blast resistance in nature. |
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| ISSN: | 2095-5421 2214-5141 |