Induction of avirulence by AVR-Pita1 in virulent U.S. field isolates of Magnaporthe oryzae
The AVR-Pita1 gene, from the Chinese isolate O-137 of Magnaporthe oryzae, is an effector that determines the efficacy of the Pi-ta rice blast resistance gene. In the present study, the avirulence function of AVR-Pita1 was induced by transformation of field isolates (TM2, ZN19, B2 and B8) that origin...
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KeAi Communications Co., Ltd.
2014-02-01
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| Series: | Crop Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514113000536 |
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| author | Yuntao Dai Eugenia Winston James C. Correll Yulin Jia |
| author_facet | Yuntao Dai Eugenia Winston James C. Correll Yulin Jia |
| author_sort | Yuntao Dai |
| collection | DOAJ |
| description | The AVR-Pita1 gene, from the Chinese isolate O-137 of Magnaporthe oryzae, is an effector that determines the efficacy of the Pi-ta rice blast resistance gene. In the present study, the avirulence function of AVR-Pita1 was induced by transformation of field isolates (TM2, ZN19, B2 and B8) that originally were collected from the U.S. and are virulent on Pi-ta-carrying rice cultivars. The presence of AVR-Pita1 from O-137 in independent transformants was detected by PCR using AVR-Pita1 specific primers and verified by DNA sequencing and Southern blot analysis using the AVR-Pita1 coding region as a probe. The results of pathogenicity assays showed that the AVR-Pita1-transformed isolates were not able to infect rice cultivars Katy and Drew carrying Pi-ta. Control isolates that were transformed with inserts lacking the AVR-Pita1 gene remained virulent. Our findings demonstrate that AVR-Pita1 can be used to induce novel gene-specific blast resistance in nature. |
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| institution | Directory Open Access Journal |
| issn | 2095-5421 2214-5141 |
| language | English |
| last_indexed | 2024-12-20T00:12:52Z |
| publishDate | 2014-02-01 |
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| series | Crop Journal |
| spelling | doaj.art-bc927fb92a9040a3951fd3c3e423f0752022-12-21T20:00:27ZengKeAi Communications Co., Ltd.Crop Journal2095-54212214-51412014-02-012119doi:10.1016/j.cj.2013.12.002Induction of avirulence by AVR-Pita1 in virulent U.S. field isolates of Magnaporthe oryzaeYuntao Dai0Eugenia Winston1James C. Correll2Yulin Jia3Department of Plant Pathology, University of Arkansas, Fayetteville, AR 72701, USA;USDA Agricultural Research Service, Dale Bumpers National Rice Research Center, 2890 Hwy 130E, Stuttgart, AR 72160, USAUSDA Agriculture Research Service, Jamie Whitten Delta States Research Center, Stoneville, MS 38776, USADepartment of Plant Pathology, University of Arkansas, Fayetteville, AR 72701, USAUSDA Agricultural Research Service, Dale Bumpers National Rice Research Center, 2890 Hwy 130E, Stuttgart, AR 72160, USAThe AVR-Pita1 gene, from the Chinese isolate O-137 of Magnaporthe oryzae, is an effector that determines the efficacy of the Pi-ta rice blast resistance gene. In the present study, the avirulence function of AVR-Pita1 was induced by transformation of field isolates (TM2, ZN19, B2 and B8) that originally were collected from the U.S. and are virulent on Pi-ta-carrying rice cultivars. The presence of AVR-Pita1 from O-137 in independent transformants was detected by PCR using AVR-Pita1 specific primers and verified by DNA sequencing and Southern blot analysis using the AVR-Pita1 coding region as a probe. The results of pathogenicity assays showed that the AVR-Pita1-transformed isolates were not able to infect rice cultivars Katy and Drew carrying Pi-ta. Control isolates that were transformed with inserts lacking the AVR-Pita1 gene remained virulent. Our findings demonstrate that AVR-Pita1 can be used to induce novel gene-specific blast resistance in nature.http://www.sciencedirect.com/science/article/pii/S2214514113000536Avirulence geneAVR-Pita1Pi-taRice blast resistance |
| spellingShingle | Yuntao Dai Eugenia Winston James C. Correll Yulin Jia Induction of avirulence by AVR-Pita1 in virulent U.S. field isolates of Magnaporthe oryzae Crop Journal Avirulence gene AVR-Pita1 Pi-ta Rice blast resistance |
| title | Induction of avirulence by AVR-Pita1 in virulent U.S. field isolates of Magnaporthe oryzae |
| title_full | Induction of avirulence by AVR-Pita1 in virulent U.S. field isolates of Magnaporthe oryzae |
| title_fullStr | Induction of avirulence by AVR-Pita1 in virulent U.S. field isolates of Magnaporthe oryzae |
| title_full_unstemmed | Induction of avirulence by AVR-Pita1 in virulent U.S. field isolates of Magnaporthe oryzae |
| title_short | Induction of avirulence by AVR-Pita1 in virulent U.S. field isolates of Magnaporthe oryzae |
| title_sort | induction of avirulence by avr pita1 in virulent u s field isolates of magnaporthe oryzae |
| topic | Avirulence gene AVR-Pita1 Pi-ta Rice blast resistance |
| url | http://www.sciencedirect.com/science/article/pii/S2214514113000536 |
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