Oncolytic Vaccinia Virus Expressing <i>Aphrocallistes vastus</i> Lectin as a Cancer Therapeutic Agent
Lectins display a variety of biological functions including insecticidal, antimicrobial, as well as antitumor activities. In this report, a gene encoding <i>Aphrocallistes vastus</i> lectin (AVL), a C-type lectin, was inserted into an oncolytic vaccinia virus vector (oncoVV) to form a re...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-06-01
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| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/17/6/363 |
| _version_ | 1828115634494373888 |
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| author | Tao Wu Yulin Xiang Tingting Liu Xue Wang Xiaoyuan Ren Ting Ye Gongchu Li |
| author_facet | Tao Wu Yulin Xiang Tingting Liu Xue Wang Xiaoyuan Ren Ting Ye Gongchu Li |
| author_sort | Tao Wu |
| collection | DOAJ |
| description | Lectins display a variety of biological functions including insecticidal, antimicrobial, as well as antitumor activities. In this report, a gene encoding <i>Aphrocallistes vastus</i> lectin (AVL), a C-type lectin, was inserted into an oncolytic vaccinia virus vector (oncoVV) to form a recombinant virus oncoVV-AVL, which showed significant in vitro antiproliferative activity in a variety of cancer cell lines. Further investigations revealed that oncoVV-AVL replicated faster than oncoVV significantly in cancer cells. Intracellular signaling elements including NF-κB2, NIK, as well as ERK were determined to be altered by oncoVV-AVL. Virus replication upregulated by AVL was completely dependent on ERK activity. Furthermore, in vivo studies showed that oncoVV-AVL elicited significant antitumor effect in colorectal cancer and liver cancer mouse models. Our study might provide insights into a novel way of the utilization of marine lectin AVL in oncolytic viral therapies. |
| first_indexed | 2024-04-11T12:44:37Z |
| format | Article |
| id | doaj.art-bc93566cc87d4b8f85e9a0549d887cce |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T12:44:37Z |
| publishDate | 2019-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-bc93566cc87d4b8f85e9a0549d887cce2022-12-22T04:23:25ZengMDPI AGMarine Drugs1660-33972019-06-0117636310.3390/md17060363md17060363Oncolytic Vaccinia Virus Expressing <i>Aphrocallistes vastus</i> Lectin as a Cancer Therapeutic AgentTao Wu0Yulin Xiang1Tingting Liu2Xue Wang3Xiaoyuan Ren4Ting Ye5Gongchu Li6Zhejiang Sci-Tech University Hangzhou Gongchu Joint Institute of Biomedicine, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaZhejiang Sci-Tech University Hangzhou Gongchu Joint Institute of Biomedicine, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaZhejiang Sci-Tech University Hangzhou Gongchu Joint Institute of Biomedicine, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaZhejiang Sci-Tech University Hangzhou Gongchu Joint Institute of Biomedicine, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaZhejiang Sci-Tech University Hangzhou Gongchu Joint Institute of Biomedicine, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaZhejiang Sci-Tech University Hangzhou Gongchu Joint Institute of Biomedicine, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaZhejiang Sci-Tech University Hangzhou Gongchu Joint Institute of Biomedicine, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaLectins display a variety of biological functions including insecticidal, antimicrobial, as well as antitumor activities. In this report, a gene encoding <i>Aphrocallistes vastus</i> lectin (AVL), a C-type lectin, was inserted into an oncolytic vaccinia virus vector (oncoVV) to form a recombinant virus oncoVV-AVL, which showed significant in vitro antiproliferative activity in a variety of cancer cell lines. Further investigations revealed that oncoVV-AVL replicated faster than oncoVV significantly in cancer cells. Intracellular signaling elements including NF-κB2, NIK, as well as ERK were determined to be altered by oncoVV-AVL. Virus replication upregulated by AVL was completely dependent on ERK activity. Furthermore, in vivo studies showed that oncoVV-AVL elicited significant antitumor effect in colorectal cancer and liver cancer mouse models. Our study might provide insights into a novel way of the utilization of marine lectin AVL in oncolytic viral therapies.https://www.mdpi.com/1660-3397/17/6/363<i>Aphrocallistes vastus</i> lectinoncolytic vaccinia virusERK |
| spellingShingle | Tao Wu Yulin Xiang Tingting Liu Xue Wang Xiaoyuan Ren Ting Ye Gongchu Li Oncolytic Vaccinia Virus Expressing <i>Aphrocallistes vastus</i> Lectin as a Cancer Therapeutic Agent Marine Drugs <i>Aphrocallistes vastus</i> lectin oncolytic vaccinia virus ERK |
| title | Oncolytic Vaccinia Virus Expressing <i>Aphrocallistes vastus</i> Lectin as a Cancer Therapeutic Agent |
| title_full | Oncolytic Vaccinia Virus Expressing <i>Aphrocallistes vastus</i> Lectin as a Cancer Therapeutic Agent |
| title_fullStr | Oncolytic Vaccinia Virus Expressing <i>Aphrocallistes vastus</i> Lectin as a Cancer Therapeutic Agent |
| title_full_unstemmed | Oncolytic Vaccinia Virus Expressing <i>Aphrocallistes vastus</i> Lectin as a Cancer Therapeutic Agent |
| title_short | Oncolytic Vaccinia Virus Expressing <i>Aphrocallistes vastus</i> Lectin as a Cancer Therapeutic Agent |
| title_sort | oncolytic vaccinia virus expressing i aphrocallistes vastus i lectin as a cancer therapeutic agent |
| topic | <i>Aphrocallistes vastus</i> lectin oncolytic vaccinia virus ERK |
| url | https://www.mdpi.com/1660-3397/17/6/363 |
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