Conversion of Animal-Derived Protein By-Products into a New Dual-Layer Nanofiber Biomaterial by Electrospinning Process
The aim of this study was to design a dual-layer wound dressing as a new fibrous biomaterial based on the valorization of animal-derived proteins. The first layer was fabricated by the deposition of poly(ethylene oxide) (PEO) loaded with keratin hydrolysate (KH) via a mono-electrospinning process on...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Fibers |
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| Online Access: | https://www.mdpi.com/2079-6439/11/10/87 |
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| author | Carmen Gaidău Maria Râpă Laura Mihaela Stefan Ecaterina Matei Andrei Constantin Berbecaru Cristian Predescu Liliana Mititelu-Tartau |
| author_facet | Carmen Gaidău Maria Râpă Laura Mihaela Stefan Ecaterina Matei Andrei Constantin Berbecaru Cristian Predescu Liliana Mititelu-Tartau |
| author_sort | Carmen Gaidău |
| collection | DOAJ |
| description | The aim of this study was to design a dual-layer wound dressing as a new fibrous biomaterial based on the valorization of animal-derived proteins. The first layer was fabricated by the deposition of poly(ethylene oxide) (PEO) loaded with keratin hydrolysate (KH) via a mono-electrospinning process onto a poly(lactic acid) (PLA) film, which was used as a support. The second layer consisted of encapsulating a bovine collagen hydrolysate (CH) into poly(vinyl pyrrolidone) (PVP) through a coaxial electrospinning process, which was added onto the previous layer. This assemblage was characterized by electronic microscopy for morphology and the controlled release of KH. In vitro biocompatibility was evaluated on the L929 (NCTC) murine fibroblasts using quantitative MTT assay and qualitative cell morphological examination after Giemsa staining. Additionally, in vivo biocompatibility methods were used to assess the impact of the biomaterial on white Swiss mice, including the evaluation of hematological, biochemical, and immunological profiles, as well as its impact on oxidative stress. The results revealed a nanofibrous structure for each layer, and the assembled product demonstrated antioxidant activity, controlled release of KH, a high degree of in vitro biocompatibility, negligible hematological and biochemical changes, and minimal impact of certain specific oxidative stress parameters compared to the use of patches with textile support. |
| first_indexed | 2024-03-10T21:15:59Z |
| format | Article |
| id | doaj.art-bc93b8489d6c444a80478f986b94a9b7 |
| institution | Directory Open Access Journal |
| issn | 2079-6439 |
| language | English |
| last_indexed | 2024-03-10T21:15:59Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fibers |
| spelling | doaj.art-bc93b8489d6c444a80478f986b94a9b72023-11-19T16:26:51ZengMDPI AGFibers2079-64392023-10-0111108710.3390/fib11100087Conversion of Animal-Derived Protein By-Products into a New Dual-Layer Nanofiber Biomaterial by Electrospinning ProcessCarmen Gaidău0Maria Râpă1Laura Mihaela Stefan2Ecaterina Matei3Andrei Constantin Berbecaru4Cristian Predescu5Liliana Mititelu-Tartau6The National Research & Development Institute for Textiles and Leather-Division Leather and Footwear Research Institute, 031251 Bucharest, RomaniaFaculty of Material Science and Engineering, National University of Science and Technology Politehnica Bucharest, 060042 Bucharest, RomaniaDepartment of Cellular and Molecular Biology, National Institute of Research and Development for Biological Sciences, 296 Splaiul Independenţei, Sector 6, 060031 Bucharest, RomaniaFaculty of Material Science and Engineering, National University of Science and Technology Politehnica Bucharest, 060042 Bucharest, RomaniaFaculty of Material Science and Engineering, National University of Science and Technology Politehnica Bucharest, 060042 Bucharest, RomaniaFaculty of Material Science and Engineering, National University of Science and Technology Politehnica Bucharest, 060042 Bucharest, RomaniaPharmacology, Clinical Pharmacology and Algesiology Department, Faculty of Medicine “Grigore T. Popa”, University of Medicine and Pharmacy, 700115 Iasi, RomaniaThe aim of this study was to design a dual-layer wound dressing as a new fibrous biomaterial based on the valorization of animal-derived proteins. The first layer was fabricated by the deposition of poly(ethylene oxide) (PEO) loaded with keratin hydrolysate (KH) via a mono-electrospinning process onto a poly(lactic acid) (PLA) film, which was used as a support. The second layer consisted of encapsulating a bovine collagen hydrolysate (CH) into poly(vinyl pyrrolidone) (PVP) through a coaxial electrospinning process, which was added onto the previous layer. This assemblage was characterized by electronic microscopy for morphology and the controlled release of KH. In vitro biocompatibility was evaluated on the L929 (NCTC) murine fibroblasts using quantitative MTT assay and qualitative cell morphological examination after Giemsa staining. Additionally, in vivo biocompatibility methods were used to assess the impact of the biomaterial on white Swiss mice, including the evaluation of hematological, biochemical, and immunological profiles, as well as its impact on oxidative stress. The results revealed a nanofibrous structure for each layer, and the assembled product demonstrated antioxidant activity, controlled release of KH, a high degree of in vitro biocompatibility, negligible hematological and biochemical changes, and minimal impact of certain specific oxidative stress parameters compared to the use of patches with textile support.https://www.mdpi.com/2079-6439/11/10/87collagen hydrolysatekeratin hydrolysatebiomaterialnanofiberselectrospinningwound dressing |
| spellingShingle | Carmen Gaidău Maria Râpă Laura Mihaela Stefan Ecaterina Matei Andrei Constantin Berbecaru Cristian Predescu Liliana Mititelu-Tartau Conversion of Animal-Derived Protein By-Products into a New Dual-Layer Nanofiber Biomaterial by Electrospinning Process Fibers collagen hydrolysate keratin hydrolysate biomaterial nanofibers electrospinning wound dressing |
| title | Conversion of Animal-Derived Protein By-Products into a New Dual-Layer Nanofiber Biomaterial by Electrospinning Process |
| title_full | Conversion of Animal-Derived Protein By-Products into a New Dual-Layer Nanofiber Biomaterial by Electrospinning Process |
| title_fullStr | Conversion of Animal-Derived Protein By-Products into a New Dual-Layer Nanofiber Biomaterial by Electrospinning Process |
| title_full_unstemmed | Conversion of Animal-Derived Protein By-Products into a New Dual-Layer Nanofiber Biomaterial by Electrospinning Process |
| title_short | Conversion of Animal-Derived Protein By-Products into a New Dual-Layer Nanofiber Biomaterial by Electrospinning Process |
| title_sort | conversion of animal derived protein by products into a new dual layer nanofiber biomaterial by electrospinning process |
| topic | collagen hydrolysate keratin hydrolysate biomaterial nanofibers electrospinning wound dressing |
| url | https://www.mdpi.com/2079-6439/11/10/87 |
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