Study of class 1 integrons in multidrug-resistant uropathogenic Escherichia coli isolated from different hospitals in Karachi
Objective(s): Escherichia coli is the key pathogen in the family producing ESBL (extended spectrum β-lactamase) and associated with community-acquired infections. Therefore, this study was planned to determine the antibiotic susceptibility pattern of uropathogenic E. coli, prevalence of the ESBL gen...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2018-10-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/article_11368_85f177b94ea4f52d026dd3c7eb3e0a49.pdf |
| _version_ | 1811246061734330368 |
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| author | Fouzia Zeeshan Khan Tehseen Nawaz Zulfiqar Mirani Saeed Khan Yasir Raza Shahana Urooj Kazmi |
| author_facet | Fouzia Zeeshan Khan Tehseen Nawaz Zulfiqar Mirani Saeed Khan Yasir Raza Shahana Urooj Kazmi |
| author_sort | Fouzia Zeeshan Khan |
| collection | DOAJ |
| description | Objective(s): Escherichia coli is the key pathogen in the family producing ESBL (extended spectrum β-lactamase) and associated with community-acquired infections. Therefore, this study was planned to determine the antibiotic susceptibility pattern of uropathogenic E. coli, prevalence of the ESBL gene group and class 1 integrons.Materials and Methods: Clinical isolates of uropathogenic E. coli were isolated from different hospitals of Karachi. Antibiotic susceptibility test was performed by Kirby-Bauer Methods. Presence of β– lactamases genes (CTX, TEM, and SHV) and integron 1 were identified by polymerase chain reaction (PCR).Results: Out of 500, 105 isolates were identified as multi-drug resistant (MDR) uropathogenic E. coli. The subject MDR isolates showed the highest resistance to aztreonam, amoxil/ clavulanic acid, ampicillin, cotrimoxazole, ceftriaxone, cefipime, and cefuroxime. Genetic analysis showed that the majority of the MDR E. coli carry CTX M1 (57.1%) followed by TEM (33.3%) and SHV (9.5%). Moreover, 79% of MDR E. coli harbored class 1 integrons, whereas all three conserved genes for class 1 integrons were present in 58% of MDR E. coli. Conclusion: This study is helpful to provide information regarding the antibiotic susceptibility pattern, distribution ESBLs and class 1 integrons among uropathogenic E. coli. |
| first_indexed | 2024-04-12T14:48:14Z |
| format | Article |
| id | doaj.art-bc94ed3414a4492aa5b2d44170804fa2 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-12T14:48:14Z |
| publishDate | 2018-10-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-bc94ed3414a4492aa5b2d44170804fa22022-12-22T03:28:34ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742018-10-0121101079108210.22038/ijbms.2018.28807.696611368Study of class 1 integrons in multidrug-resistant uropathogenic Escherichia coli isolated from different hospitals in KarachiFouzia Zeeshan Khan0Tehseen Nawaz1Zulfiqar Mirani2Saeed Khan3Yasir Raza4Shahana Urooj Kazmi5Dow University of Health Sciences, Karachi, PakistanBarrett Hodgson University, Karachi, PakistanPakistan Council of Scientific & Industrial Research Laboratories Complex, Karachi, PakistanDepartment of Microbiology, University of Karachi, Karachi, PakistanDada Bhoy Institute of Higher Education Commission, Karachi, PakistanDada Bhoy Institute of Higher Education Commission, Karachi, PakistanObjective(s): Escherichia coli is the key pathogen in the family producing ESBL (extended spectrum β-lactamase) and associated with community-acquired infections. Therefore, this study was planned to determine the antibiotic susceptibility pattern of uropathogenic E. coli, prevalence of the ESBL gene group and class 1 integrons.Materials and Methods: Clinical isolates of uropathogenic E. coli were isolated from different hospitals of Karachi. Antibiotic susceptibility test was performed by Kirby-Bauer Methods. Presence of β– lactamases genes (CTX, TEM, and SHV) and integron 1 were identified by polymerase chain reaction (PCR).Results: Out of 500, 105 isolates were identified as multi-drug resistant (MDR) uropathogenic E. coli. The subject MDR isolates showed the highest resistance to aztreonam, amoxil/ clavulanic acid, ampicillin, cotrimoxazole, ceftriaxone, cefipime, and cefuroxime. Genetic analysis showed that the majority of the MDR E. coli carry CTX M1 (57.1%) followed by TEM (33.3%) and SHV (9.5%). Moreover, 79% of MDR E. coli harbored class 1 integrons, whereas all three conserved genes for class 1 integrons were present in 58% of MDR E. coli. Conclusion: This study is helpful to provide information regarding the antibiotic susceptibility pattern, distribution ESBLs and class 1 integrons among uropathogenic E. coli.http://ijbms.mums.ac.ir/article_11368_85f177b94ea4f52d026dd3c7eb3e0a49.pdfClass 1 integronsESBLsMDR E. coliMultidrug resistanceUropathogenic E. coli |
| spellingShingle | Fouzia Zeeshan Khan Tehseen Nawaz Zulfiqar Mirani Saeed Khan Yasir Raza Shahana Urooj Kazmi Study of class 1 integrons in multidrug-resistant uropathogenic Escherichia coli isolated from different hospitals in Karachi Iranian Journal of Basic Medical Sciences Class 1 integrons ESBLs MDR E. coli Multidrug resistance Uropathogenic E. coli |
| title | Study of class 1 integrons in multidrug-resistant uropathogenic Escherichia coli isolated from different hospitals in Karachi |
| title_full | Study of class 1 integrons in multidrug-resistant uropathogenic Escherichia coli isolated from different hospitals in Karachi |
| title_fullStr | Study of class 1 integrons in multidrug-resistant uropathogenic Escherichia coli isolated from different hospitals in Karachi |
| title_full_unstemmed | Study of class 1 integrons in multidrug-resistant uropathogenic Escherichia coli isolated from different hospitals in Karachi |
| title_short | Study of class 1 integrons in multidrug-resistant uropathogenic Escherichia coli isolated from different hospitals in Karachi |
| title_sort | study of class 1 integrons in multidrug resistant uropathogenic escherichia coli isolated from different hospitals in karachi |
| topic | Class 1 integrons ESBLs MDR E. coli Multidrug resistance Uropathogenic E. coli |
| url | http://ijbms.mums.ac.ir/article_11368_85f177b94ea4f52d026dd3c7eb3e0a49.pdf |
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