5-Aminolevulinic acid/sodium ferrous citrate improves the quality of heat-stressed bovine oocytes by reducing oxidative stress
A high temperature-humidity index during summer has deleterious effects on mitochondrial function, reducing oocyte developmental competence. 5-Aminolevulinic acid (5-ALA) and sodium ferrous citrate (SFC) are both known to support mitochondrial function and have strong anti-oxidant and anti-apoptotic...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The Society for Reproduction and Development
2023-08-01
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| Series: | The Journal of Reproduction and Development |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jrd/69/5/69_2023-038/_pdf/-char/en |
| _version_ | 1797643149771800576 |
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| author | Omnia ELGENDY Go KITAHARA Kentaro YAMADA Shin TANIGUCHI Takeshi OSAWA |
| author_facet | Omnia ELGENDY Go KITAHARA Kentaro YAMADA Shin TANIGUCHI Takeshi OSAWA |
| author_sort | Omnia ELGENDY |
| collection | DOAJ |
| description | A high temperature-humidity index during summer has deleterious effects on mitochondrial function, reducing oocyte developmental competence. 5-Aminolevulinic acid (5-ALA) and sodium ferrous citrate (SFC) are both known to support mitochondrial function and have strong anti-oxidant and anti-apoptotic activities. This study aimed to determine the mechanism of action of 5-ALA/SFC on oocyte quality. Bovine oocytes were collected from medium-sized follicles during summer (July–September, temperature-humidity index:76.6), cultured with 0, 1, 2, 4, and 8 µM 5-ALA with SFC at a molar ratio of 1:0.125, fertilized, and cultured for 10 days. The addition of 8/1 µM 5-ALA/SFC had a deleterious effect on oocyte cleavage rate in comparison with control oocytes, but did not affect the blastocyst rate, while 1/0.125 µM 5-ALA/SFC had a significantly higher increase in blastocyst rate than 8/1 µM 5-ALA/SFC. The addition of 1/0.125 and 2/0.25 µM 5-ALA/SFC improved oocyte quality by increasing the mitochondrial distribution pattern and metaphase-II oocytes, reducing reactive oxygen species and upregulating nuclear factor erythroid-2-related factor 2, heme oxygenase-1, and superoxide dismutase-1 in oocytes, and nuclear factor erythroid-2-related factor 2 and mitochondrial transcription factor A in cumulus cells. These results indicate that 1/0.125 and 2/0.25 µM 5-ALA/SFC may support oocyte quality and developmental competence and provide anti-oxidant actions in cumulus-oocyte complexes. |
| first_indexed | 2024-03-11T14:11:34Z |
| format | Article |
| id | doaj.art-bc9ab683d6024222a85edc4f763c261f |
| institution | Directory Open Access Journal |
| issn | 0916-8818 1348-4400 |
| language | English |
| last_indexed | 2024-03-11T14:11:34Z |
| publishDate | 2023-08-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj.art-bc9ab683d6024222a85edc4f763c261f2023-11-02T00:05:33ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002023-08-0169526126910.1262/jrd.2023-038jrd5-Aminolevulinic acid/sodium ferrous citrate improves the quality of heat-stressed bovine oocytes by reducing oxidative stressOmnia ELGENDY0Go KITAHARA1Kentaro YAMADA2Shin TANIGUCHI3Takeshi OSAWA4Graduate School of Medicine and Veterinary Medicine, University of Miyazaki, Miyazaki 889-2192, JapanGraduate School of Medicine and Veterinary Medicine, University of Miyazaki, Miyazaki 889-2192, JapanGraduate School of Medicine and Veterinary Medicine, University of Miyazaki, Miyazaki 889-2192, JapanOne Health Business Department, Neopharma Japan Co., Ltd., Tokyo 102-0071, JapanGraduate School of Medicine and Veterinary Medicine, University of Miyazaki, Miyazaki 889-2192, JapanA high temperature-humidity index during summer has deleterious effects on mitochondrial function, reducing oocyte developmental competence. 5-Aminolevulinic acid (5-ALA) and sodium ferrous citrate (SFC) are both known to support mitochondrial function and have strong anti-oxidant and anti-apoptotic activities. This study aimed to determine the mechanism of action of 5-ALA/SFC on oocyte quality. Bovine oocytes were collected from medium-sized follicles during summer (July–September, temperature-humidity index:76.6), cultured with 0, 1, 2, 4, and 8 µM 5-ALA with SFC at a molar ratio of 1:0.125, fertilized, and cultured for 10 days. The addition of 8/1 µM 5-ALA/SFC had a deleterious effect on oocyte cleavage rate in comparison with control oocytes, but did not affect the blastocyst rate, while 1/0.125 µM 5-ALA/SFC had a significantly higher increase in blastocyst rate than 8/1 µM 5-ALA/SFC. The addition of 1/0.125 and 2/0.25 µM 5-ALA/SFC improved oocyte quality by increasing the mitochondrial distribution pattern and metaphase-II oocytes, reducing reactive oxygen species and upregulating nuclear factor erythroid-2-related factor 2, heme oxygenase-1, and superoxide dismutase-1 in oocytes, and nuclear factor erythroid-2-related factor 2 and mitochondrial transcription factor A in cumulus cells. These results indicate that 1/0.125 and 2/0.25 µM 5-ALA/SFC may support oocyte quality and developmental competence and provide anti-oxidant actions in cumulus-oocyte complexes.https://www.jstage.jst.go.jp/article/jrd/69/5/69_2023-038/_pdf/-char/en5-aminolevulinic acid/sodium ferrous citrate (5-ala/sfc)anti-oxidant pathwaybovine oocyteheat stressmitochondria |
| spellingShingle | Omnia ELGENDY Go KITAHARA Kentaro YAMADA Shin TANIGUCHI Takeshi OSAWA 5-Aminolevulinic acid/sodium ferrous citrate improves the quality of heat-stressed bovine oocytes by reducing oxidative stress The Journal of Reproduction and Development 5-aminolevulinic acid/sodium ferrous citrate (5-ala/sfc) anti-oxidant pathway bovine oocyte heat stress mitochondria |
| title | 5-Aminolevulinic acid/sodium ferrous citrate improves the quality of heat-stressed bovine oocytes by reducing oxidative stress |
| title_full | 5-Aminolevulinic acid/sodium ferrous citrate improves the quality of heat-stressed bovine oocytes by reducing oxidative stress |
| title_fullStr | 5-Aminolevulinic acid/sodium ferrous citrate improves the quality of heat-stressed bovine oocytes by reducing oxidative stress |
| title_full_unstemmed | 5-Aminolevulinic acid/sodium ferrous citrate improves the quality of heat-stressed bovine oocytes by reducing oxidative stress |
| title_short | 5-Aminolevulinic acid/sodium ferrous citrate improves the quality of heat-stressed bovine oocytes by reducing oxidative stress |
| title_sort | 5 aminolevulinic acid sodium ferrous citrate improves the quality of heat stressed bovine oocytes by reducing oxidative stress |
| topic | 5-aminolevulinic acid/sodium ferrous citrate (5-ala/sfc) anti-oxidant pathway bovine oocyte heat stress mitochondria |
| url | https://www.jstage.jst.go.jp/article/jrd/69/5/69_2023-038/_pdf/-char/en |
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