Untargeted Metabolomics and Polyamine Profiling in Serum before and after Surgery in Colorectal Cancer Patients
Colorectal cancer is one of the most prevalent cancers in Korea and globally. In this study, we aimed to characterize the differential serum metabolomic profiles between pre-operative and post-operative patients with colorectal cancer. To investigate the significant metabolites and metabolic pathway...
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| Format: | Article |
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MDPI AG
2020-11-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/10/12/487 |
| _version_ | 1797546548772470784 |
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| author | Yu Ra Lee Ki-Yong An Justin Jeon Nam Kyu Kim Ji Won Lee Jongki Hong Bong Chul Chung |
| author_facet | Yu Ra Lee Ki-Yong An Justin Jeon Nam Kyu Kim Ji Won Lee Jongki Hong Bong Chul Chung |
| author_sort | Yu Ra Lee |
| collection | DOAJ |
| description | Colorectal cancer is one of the most prevalent cancers in Korea and globally. In this study, we aimed to characterize the differential serum metabolomic profiles between pre-operative and post-operative patients with colorectal cancer. To investigate the significant metabolites and metabolic pathways associated with colorectal cancer, we analyzed serum samples from 68 patients (aged 20–71, mean 57.57 years). Untargeted and targeted metabolomics profiling in patients with colorectal cancer were performed using liquid chromatography-mass spectrometry. Untargeted analysis identified differences in sphingolipid metabolism, steroid biosynthesis, and arginine and proline metabolism in pre- and post-operative patients with colorectal cancer. We then performed quantitative target profiling of polyamines, synthesized from arginine and proline metabolism, to identify potential polyamines that may serve as effective biomarkers for colorectal cancer. Results indicate a significantly reduced serum concentration of putrescine in post-operative patients compared to pre-operative patients. Our metabolomics approach provided insights into the physiological alterations in patients with colorectal cancer after surgery. |
| first_indexed | 2024-03-10T14:32:22Z |
| format | Article |
| id | doaj.art-bca18388f109457b831c28ab5e38f661 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T14:32:22Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-bca18388f109457b831c28ab5e38f6612023-11-20T22:32:21ZengMDPI AGMetabolites2218-19892020-11-01101248710.3390/metabo10120487Untargeted Metabolomics and Polyamine Profiling in Serum before and after Surgery in Colorectal Cancer PatientsYu Ra Lee0Ki-Yong An1Justin Jeon2Nam Kyu Kim3Ji Won Lee4Jongki Hong5Bong Chul Chung6Molecular Recognition Research Center, Korea Institute of Science and Technology, Seoul 02792, KoreaFaculty of Kinesiology, Sport and Recreation, University of Alberta, Edmonton, AB T6G 2R3, CanadaDepartment of Sport Industry, Yonsei University, Seoul 03722, KoreaDepartment of Surgery, Yonsei University College of Medicine, Seoul 03722, KoreaDepartment of Family Medicine, Yonsei University College of Medicine, Seoul 06273, KoreaKHU-KIST Department of Converging Science and Technology, Kyung Hee University, Seoul 02447, KoreaMolecular Recognition Research Center, Korea Institute of Science and Technology, Seoul 02792, KoreaColorectal cancer is one of the most prevalent cancers in Korea and globally. In this study, we aimed to characterize the differential serum metabolomic profiles between pre-operative and post-operative patients with colorectal cancer. To investigate the significant metabolites and metabolic pathways associated with colorectal cancer, we analyzed serum samples from 68 patients (aged 20–71, mean 57.57 years). Untargeted and targeted metabolomics profiling in patients with colorectal cancer were performed using liquid chromatography-mass spectrometry. Untargeted analysis identified differences in sphingolipid metabolism, steroid biosynthesis, and arginine and proline metabolism in pre- and post-operative patients with colorectal cancer. We then performed quantitative target profiling of polyamines, synthesized from arginine and proline metabolism, to identify potential polyamines that may serve as effective biomarkers for colorectal cancer. Results indicate a significantly reduced serum concentration of putrescine in post-operative patients compared to pre-operative patients. Our metabolomics approach provided insights into the physiological alterations in patients with colorectal cancer after surgery.https://www.mdpi.com/2218-1989/10/12/487colorectal cancerliquid chromatography-mass spectrometrypolyaminetumorectomymetabolomicsbiomarker |
| spellingShingle | Yu Ra Lee Ki-Yong An Justin Jeon Nam Kyu Kim Ji Won Lee Jongki Hong Bong Chul Chung Untargeted Metabolomics and Polyamine Profiling in Serum before and after Surgery in Colorectal Cancer Patients Metabolites colorectal cancer liquid chromatography-mass spectrometry polyamine tumorectomy metabolomics biomarker |
| title | Untargeted Metabolomics and Polyamine Profiling in Serum before and after Surgery in Colorectal Cancer Patients |
| title_full | Untargeted Metabolomics and Polyamine Profiling in Serum before and after Surgery in Colorectal Cancer Patients |
| title_fullStr | Untargeted Metabolomics and Polyamine Profiling in Serum before and after Surgery in Colorectal Cancer Patients |
| title_full_unstemmed | Untargeted Metabolomics and Polyamine Profiling in Serum before and after Surgery in Colorectal Cancer Patients |
| title_short | Untargeted Metabolomics and Polyamine Profiling in Serum before and after Surgery in Colorectal Cancer Patients |
| title_sort | untargeted metabolomics and polyamine profiling in serum before and after surgery in colorectal cancer patients |
| topic | colorectal cancer liquid chromatography-mass spectrometry polyamine tumorectomy metabolomics biomarker |
| url | https://www.mdpi.com/2218-1989/10/12/487 |
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