miRNA expression in anaplastic thyroid carcinomas.
Anaplastic thyroid carcinoma (ATC) is the most lethal form of thyroid neoplasia and represents an end stage of thyroid tumor progression. No effective treatment exists so far. In this study, we analyzed the miRNA expression profiles of 11 ATC by microarrays and their relationship with the mRNA expre...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4143225?pdf=render |
| _version_ | 1818692910127775744 |
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| author | Aline Hébrant Sébastien Floor Manuel Saiselet Aline Antoniou Alice Desbuleux Bérengère Snyers Caroline La Nicolas de Saint Aubain Emmanuelle Leteurtre Guy Andry Carine Maenhaut |
| author_facet | Aline Hébrant Sébastien Floor Manuel Saiselet Aline Antoniou Alice Desbuleux Bérengère Snyers Caroline La Nicolas de Saint Aubain Emmanuelle Leteurtre Guy Andry Carine Maenhaut |
| author_sort | Aline Hébrant |
| collection | DOAJ |
| description | Anaplastic thyroid carcinoma (ATC) is the most lethal form of thyroid neoplasia and represents an end stage of thyroid tumor progression. No effective treatment exists so far. In this study, we analyzed the miRNA expression profiles of 11 ATC by microarrays and their relationship with the mRNA expression profiles of the same 11 ATC samples. ATC show distinct miRNA expression profiles compared to other less aggressive thyroid tumor types. ATC show 18 commonly deregulated miRNA compared to normal thyroid tissue (17 downregulated and 1 upregulated miRNA). First, the analysis of a combined approach of the mRNA gene expression and of the bioinformatically predicted mRNA targets of the deregulated miRNA suggested a role for these regulations in the epithelial to mesenchymal transition (EMT) process in ATC. Second, the direct interaction between one of the upregulated mRNA target, the LOX gene which is an EMT key player, and a downregulated miRNA, the miR-29a, was experimentally validated by a luciferase assay in HEK cell. Third, we confirmed that the ATC tissue is composed of about 50% of tumor associated macrophages (TAM) and suggested, by taking into account our data and published data, their most likely direct or paracrine intercommunication between them and the thyroid tumor cells, amplifying the tumor aggressiveness. Finally, we demonstrated by in situ hybridization a specific thyrocyte localization of 3 of the deregulated miRNA: let-7g, miR-29a and miR-30e and we pointed out the importance of identifying the cell type localization before drawing any conclusion on the physiopathological role of a given gene. |
| first_indexed | 2024-12-17T13:05:17Z |
| format | Article |
| id | doaj.art-bca2eba4b0a14d2bbd7f8825728d5865 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T13:05:17Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-bca2eba4b0a14d2bbd7f8825728d58652022-12-21T21:47:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0198e10387110.1371/journal.pone.0103871miRNA expression in anaplastic thyroid carcinomas.Aline HébrantSébastien FloorManuel SaiseletAline AntoniouAlice DesbuleuxBérengère SnyersCaroline LaNicolas de Saint AubainEmmanuelle LeteurtreGuy AndryCarine MaenhautAnaplastic thyroid carcinoma (ATC) is the most lethal form of thyroid neoplasia and represents an end stage of thyroid tumor progression. No effective treatment exists so far. In this study, we analyzed the miRNA expression profiles of 11 ATC by microarrays and their relationship with the mRNA expression profiles of the same 11 ATC samples. ATC show distinct miRNA expression profiles compared to other less aggressive thyroid tumor types. ATC show 18 commonly deregulated miRNA compared to normal thyroid tissue (17 downregulated and 1 upregulated miRNA). First, the analysis of a combined approach of the mRNA gene expression and of the bioinformatically predicted mRNA targets of the deregulated miRNA suggested a role for these regulations in the epithelial to mesenchymal transition (EMT) process in ATC. Second, the direct interaction between one of the upregulated mRNA target, the LOX gene which is an EMT key player, and a downregulated miRNA, the miR-29a, was experimentally validated by a luciferase assay in HEK cell. Third, we confirmed that the ATC tissue is composed of about 50% of tumor associated macrophages (TAM) and suggested, by taking into account our data and published data, their most likely direct or paracrine intercommunication between them and the thyroid tumor cells, amplifying the tumor aggressiveness. Finally, we demonstrated by in situ hybridization a specific thyrocyte localization of 3 of the deregulated miRNA: let-7g, miR-29a and miR-30e and we pointed out the importance of identifying the cell type localization before drawing any conclusion on the physiopathological role of a given gene.http://europepmc.org/articles/PMC4143225?pdf=render |
| spellingShingle | Aline Hébrant Sébastien Floor Manuel Saiselet Aline Antoniou Alice Desbuleux Bérengère Snyers Caroline La Nicolas de Saint Aubain Emmanuelle Leteurtre Guy Andry Carine Maenhaut miRNA expression in anaplastic thyroid carcinomas. PLoS ONE |
| title | miRNA expression in anaplastic thyroid carcinomas. |
| title_full | miRNA expression in anaplastic thyroid carcinomas. |
| title_fullStr | miRNA expression in anaplastic thyroid carcinomas. |
| title_full_unstemmed | miRNA expression in anaplastic thyroid carcinomas. |
| title_short | miRNA expression in anaplastic thyroid carcinomas. |
| title_sort | mirna expression in anaplastic thyroid carcinomas |
| url | http://europepmc.org/articles/PMC4143225?pdf=render |
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