Gene expression analysis of the microdissected trophoblast layer of human placenta after the spontaneous onset of labor.
BACKGROUND:Despite increasing evidence that human parturition is associated with alteration in gene expression in the uteroplacental unit, the precise mechanisms that elicit spontaneous term labor in humans remain unknown. Our goal in this study was to compare the mRNA expression pattern of the trop...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3798298?pdf=render |
| _version_ | 1819262431979896832 |
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| author | Soo Hyun Kim Sung Han Shim Se Ra Sung Kyung A Lee Jung Yun Shim Dong Hyun Cha Kyoung Jin Lee |
| author_facet | Soo Hyun Kim Sung Han Shim Se Ra Sung Kyung A Lee Jung Yun Shim Dong Hyun Cha Kyoung Jin Lee |
| author_sort | Soo Hyun Kim |
| collection | DOAJ |
| description | BACKGROUND:Despite increasing evidence that human parturition is associated with alteration in gene expression in the uteroplacental unit, the precise mechanisms that elicit spontaneous term labor in humans remain unknown. Our goal in this study was to compare the mRNA expression pattern of the trophoblast layer of normal term placenta between women who had given natural birth (labor group) and those who had undergone an elective cesarean section without labor (non-labor group). METHODS:We collected placental tissue samples from six pregnant women after term vaginal deliveries (labor group) and from six pregnant women after scheduled Cesarean sections (non-labor group). Frozen sections were made immediately after placental delivery. Because the placenta is a heterogeneous tissue composed of several cell types, we used laser capture microdissection to separate the trophoblast layer from the rest of the placental tissues. RESULTS:A number of genes were differentially expressed in the trophoblast layer when the labor and non-labor groups were compared. The expression of SIRT1, KAP1, and CRH was significantly lower in the trophoblast layer of the labor group than of the non-labor group. The expression of IL-1b, NF-kB1 and TLR 8 in the labor group was significantly higher than that in the non-labor group. CONCLUSIONS:Human term labor may be closely associated with inflammatory response. We suggest that downregulation of SIRT1, KAP1, and CRH gene expression in the trophoblast may play a key role in parturition and initiation of labor in pregnant human females. |
| first_indexed | 2024-12-23T19:57:36Z |
| format | Article |
| id | doaj.art-bca9563e21724a11903e1f92a97ec2c4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T19:57:36Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-bca9563e21724a11903e1f92a97ec2c42022-12-21T17:33:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01810e7764810.1371/journal.pone.0077648Gene expression analysis of the microdissected trophoblast layer of human placenta after the spontaneous onset of labor.Soo Hyun KimSung Han ShimSe Ra SungKyung A LeeJung Yun ShimDong Hyun ChaKyoung Jin LeeBACKGROUND:Despite increasing evidence that human parturition is associated with alteration in gene expression in the uteroplacental unit, the precise mechanisms that elicit spontaneous term labor in humans remain unknown. Our goal in this study was to compare the mRNA expression pattern of the trophoblast layer of normal term placenta between women who had given natural birth (labor group) and those who had undergone an elective cesarean section without labor (non-labor group). METHODS:We collected placental tissue samples from six pregnant women after term vaginal deliveries (labor group) and from six pregnant women after scheduled Cesarean sections (non-labor group). Frozen sections were made immediately after placental delivery. Because the placenta is a heterogeneous tissue composed of several cell types, we used laser capture microdissection to separate the trophoblast layer from the rest of the placental tissues. RESULTS:A number of genes were differentially expressed in the trophoblast layer when the labor and non-labor groups were compared. The expression of SIRT1, KAP1, and CRH was significantly lower in the trophoblast layer of the labor group than of the non-labor group. The expression of IL-1b, NF-kB1 and TLR 8 in the labor group was significantly higher than that in the non-labor group. CONCLUSIONS:Human term labor may be closely associated with inflammatory response. We suggest that downregulation of SIRT1, KAP1, and CRH gene expression in the trophoblast may play a key role in parturition and initiation of labor in pregnant human females.http://europepmc.org/articles/PMC3798298?pdf=render |
| spellingShingle | Soo Hyun Kim Sung Han Shim Se Ra Sung Kyung A Lee Jung Yun Shim Dong Hyun Cha Kyoung Jin Lee Gene expression analysis of the microdissected trophoblast layer of human placenta after the spontaneous onset of labor. PLoS ONE |
| title | Gene expression analysis of the microdissected trophoblast layer of human placenta after the spontaneous onset of labor. |
| title_full | Gene expression analysis of the microdissected trophoblast layer of human placenta after the spontaneous onset of labor. |
| title_fullStr | Gene expression analysis of the microdissected trophoblast layer of human placenta after the spontaneous onset of labor. |
| title_full_unstemmed | Gene expression analysis of the microdissected trophoblast layer of human placenta after the spontaneous onset of labor. |
| title_short | Gene expression analysis of the microdissected trophoblast layer of human placenta after the spontaneous onset of labor. |
| title_sort | gene expression analysis of the microdissected trophoblast layer of human placenta after the spontaneous onset of labor |
| url | http://europepmc.org/articles/PMC3798298?pdf=render |
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