Immobilisation of endoinulinase on polyhydroxybutyrate microfibers
Due to the health benefits associated with the consumption of prebiotic short-chain fructooligosaccharides (sc-FOS) and inulooligosaccharides (IOS), there is increased interest in the use of these compounds in food products. We have developed a new biocatalyst for the production of FOS and IOS by in...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2016-12-01
|
| Series: | Czech Journal of Food Sciences |
| Subjects: | |
| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-201606-0010_immobilisation-of-endoinulinase-on-polyhydroxybutyrate-microfibers.php |
| _version_ | 1797899631092301824 |
|---|---|
| author | Milos Beran Jitka Pinkrová Marian Urban Josef Drahorád |
| author_facet | Milos Beran Jitka Pinkrová Marian Urban Josef Drahorád |
| author_sort | Milos Beran |
| collection | DOAJ |
| description | Due to the health benefits associated with the consumption of prebiotic short-chain fructooligosaccharides (sc-FOS) and inulooligosaccharides (IOS), there is increased interest in the use of these compounds in food products. We have developed a new biocatalyst for the production of FOS and IOS by inulin hydrolysis. Endoinulinase from Aspergillus niger Inulinase® Novozym 960 (Novozymes) was immobilised on polyhydroxybutyrate (PHB) nanofibres and microfibres by hydrophobic interactions. The PHB fibres were prepared by centrifugal spinning. FOS and IOS profiles were determined by ion-exchange chromatography with the Rezex RSO-Oligosaccharide column. The biocatalyst had very good activity and stability after repeated applications. It can be used in biocatalytic membrane reactors for the production of prebiotic oligosaccharides. |
| first_indexed | 2024-04-10T08:33:03Z |
| format | Article |
| id | doaj.art-bcaacfe40378454c8a4a9e885b6d3251 |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:33:03Z |
| publishDate | 2016-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-bcaacfe40378454c8a4a9e885b6d32512023-02-23T03:28:20ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172016-12-0134654154610.17221/72/2016-CJFScjf-201606-0010Immobilisation of endoinulinase on polyhydroxybutyrate microfibersMilos Beran0Jitka Pinkrová1Marian Urban2Josef Drahorád3Department of Quality Features and Microbial Products, Food Research Institute Prague, Prague, Czech RepublicDepartment of Quality Features and Microbial Products, Food Research Institute Prague, Prague, Czech RepublicDepartment of Quality Features and Microbial Products, Food Research Institute Prague, Prague, Czech RepublicDepartment of Quality Features and Microbial Products, Food Research Institute Prague, Prague, Czech RepublicDue to the health benefits associated with the consumption of prebiotic short-chain fructooligosaccharides (sc-FOS) and inulooligosaccharides (IOS), there is increased interest in the use of these compounds in food products. We have developed a new biocatalyst for the production of FOS and IOS by inulin hydrolysis. Endoinulinase from Aspergillus niger Inulinase® Novozym 960 (Novozymes) was immobilised on polyhydroxybutyrate (PHB) nanofibres and microfibres by hydrophobic interactions. The PHB fibres were prepared by centrifugal spinning. FOS and IOS profiles were determined by ion-exchange chromatography with the Rezex RSO-Oligosaccharide column. The biocatalyst had very good activity and stability after repeated applications. It can be used in biocatalytic membrane reactors for the production of prebiotic oligosaccharides.https://cjfs.agriculturejournals.cz/artkey/cjf-201606-0010_immobilisation-of-endoinulinase-on-polyhydroxybutyrate-microfibers.phpinulinfructooligosaccharideinulooligosaccharideinulinasenanofibresphb |
| spellingShingle | Milos Beran Jitka Pinkrová Marian Urban Josef Drahorád Immobilisation of endoinulinase on polyhydroxybutyrate microfibers Czech Journal of Food Sciences inulin fructooligosaccharide inulooligosaccharide inulinase nanofibres phb |
| title | Immobilisation of endoinulinase on polyhydroxybutyrate microfibers |
| title_full | Immobilisation of endoinulinase on polyhydroxybutyrate microfibers |
| title_fullStr | Immobilisation of endoinulinase on polyhydroxybutyrate microfibers |
| title_full_unstemmed | Immobilisation of endoinulinase on polyhydroxybutyrate microfibers |
| title_short | Immobilisation of endoinulinase on polyhydroxybutyrate microfibers |
| title_sort | immobilisation of endoinulinase on polyhydroxybutyrate microfibers |
| topic | inulin fructooligosaccharide inulooligosaccharide inulinase nanofibres phb |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-201606-0010_immobilisation-of-endoinulinase-on-polyhydroxybutyrate-microfibers.php |
| work_keys_str_mv | AT milosberan immobilisationofendoinulinaseonpolyhydroxybutyratemicrofibers AT jitkapinkrova immobilisationofendoinulinaseonpolyhydroxybutyratemicrofibers AT marianurban immobilisationofendoinulinaseonpolyhydroxybutyratemicrofibers AT josefdrahorad immobilisationofendoinulinaseonpolyhydroxybutyratemicrofibers |