A Simple and Straightforward Method for Activity Measurement of Carbonic Anhydrases

Carbonic anhydrase (CA) is an enzyme of high interest due to its high implications relative to the medical and environmental sectors. In the current paper, an enzyme assay for the determination of CA activity is proposed and it is characterized by its simplicity and high practicability. It permits t...

Full description

Bibliographic Details
Main Authors: Werner Fuchs, Franziska Steger, Johanna Reich, Doris Ribitsch, Simon K.-M. R. Rittmann, Günther Bochmann
Format: Article
Language:English
Published: MDPI AG 2021-07-01
Series:Catalysts
Subjects:
Online Access:https://www.mdpi.com/2073-4344/11/7/819
Description
Summary:Carbonic anhydrase (CA) is an enzyme of high interest due to its high implications relative to the medical and environmental sectors. In the current paper, an enzyme assay for the determination of CA activity is proposed and it is characterized by its simplicity and high practicability. It permits the straightforward comparison of CAs performance in physiological conditions. The methodology and the theoretical background of the evaluation method are explained in detail. Moreover, the presumed advantages over alternative assays are discussed. The assay has proven to be particularly useful for the screening of CA activity with respect to their application in CO<sub>2</sub>, capturing processes for further utilization or storage.
ISSN:2073-4344