Germination, Physicochemical Properties, and Antioxidant Enzyme Activities in Kangkong (<i>Ipomoea aquatica</i> Forssk.) Seeds as Affected by Dielectric Barrier Discharge Plasma
Dielectric barrier discharge (DBD) plasma has been utilized as a sustainable technology to enhance seed germination in various plant species. The objective of this research was to identify the mechanism of physicochemical properties and antioxidant enzyme activities to promote kangkong (<i>Ipo...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/12/1269 |
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| author | Prapasiri Ongrak Nopporn Poolyarat Suebsak Suksaengpanomrung Kamtorn Saidarasamoot Yaowapha Jirakiattikul Panumart Rithichai |
| author_facet | Prapasiri Ongrak Nopporn Poolyarat Suebsak Suksaengpanomrung Kamtorn Saidarasamoot Yaowapha Jirakiattikul Panumart Rithichai |
| author_sort | Prapasiri Ongrak |
| collection | DOAJ |
| description | Dielectric barrier discharge (DBD) plasma has been utilized as a sustainable technology to enhance seed germination in various plant species. The objective of this research was to identify the mechanism of physicochemical properties and antioxidant enzyme activities to promote kangkong (<i>Ipomoea aquatica</i> Forssk.) seed germination using different durations of DBD plasma treatments. Seeds were exposed to atmospheric DBD plasma from 5 to 20 min, compared to non-treated seeds as the control. According to SEM images, the seed surface had cracks and grew wider as a result of the prolonged DBD plasma treatments. A longer DBD plasma treatment exhibited a lower water contact angle and increased water absorption. DBD plasma treatments strongly improved germination percentages and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) contents. Seeds treated with DBD plasma for 20 min showed the highest malondialdehyde (MDA) content and the lowest field emergence. Catalase (CAT) activity increased under DBD plasma treatments for 5 and 10 min. Ascorbate peroxidase (APX) and superoxide dismutase (SOD) activities were not statistically different among the treatments. This finding suggested that DBD plasma treatments stimulated the germination of kangkong seeds by modifying the seed surface, and upregulating H<sub>2</sub>O<sub>2</sub> content and CAT activity. Five minutes was an appropriate time to treat DBD plasma. |
| first_indexed | 2024-03-08T20:43:03Z |
| format | Article |
| id | doaj.art-bcae2400a8ba41c69ac1e19789827bff |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-08T20:43:03Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-bcae2400a8ba41c69ac1e19789827bff2023-12-22T14:12:26ZengMDPI AGHorticulturae2311-75242023-11-01912126910.3390/horticulturae9121269Germination, Physicochemical Properties, and Antioxidant Enzyme Activities in Kangkong (<i>Ipomoea aquatica</i> Forssk.) Seeds as Affected by Dielectric Barrier Discharge PlasmaPrapasiri Ongrak0Nopporn Poolyarat1Suebsak Suksaengpanomrung2Kamtorn Saidarasamoot3Yaowapha Jirakiattikul4Panumart Rithichai5Department of Biotechnology, Faculty of Science and Technology, Thammasat University, Pathum Thani 12120, ThailandCenter of Advanced Nuclear Technology, Thailand Institute of Nuclear Technology (Public Organization), Nakhon Nayok 26120, ThailandCenter of Advanced Nuclear Technology, Thailand Institute of Nuclear Technology (Public Organization), Nakhon Nayok 26120, ThailandCenter of Advanced Nuclear Technology, Thailand Institute of Nuclear Technology (Public Organization), Nakhon Nayok 26120, ThailandDepartment of Agricultural Technology, Faculty of Science and Technology, Thammasat University, Pathum Thani 12120, ThailandDepartment of Agricultural Technology, Faculty of Science and Technology, Thammasat University, Pathum Thani 12120, ThailandDielectric barrier discharge (DBD) plasma has been utilized as a sustainable technology to enhance seed germination in various plant species. The objective of this research was to identify the mechanism of physicochemical properties and antioxidant enzyme activities to promote kangkong (<i>Ipomoea aquatica</i> Forssk.) seed germination using different durations of DBD plasma treatments. Seeds were exposed to atmospheric DBD plasma from 5 to 20 min, compared to non-treated seeds as the control. According to SEM images, the seed surface had cracks and grew wider as a result of the prolonged DBD plasma treatments. A longer DBD plasma treatment exhibited a lower water contact angle and increased water absorption. DBD plasma treatments strongly improved germination percentages and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) contents. Seeds treated with DBD plasma for 20 min showed the highest malondialdehyde (MDA) content and the lowest field emergence. Catalase (CAT) activity increased under DBD plasma treatments for 5 and 10 min. Ascorbate peroxidase (APX) and superoxide dismutase (SOD) activities were not statistically different among the treatments. This finding suggested that DBD plasma treatments stimulated the germination of kangkong seeds by modifying the seed surface, and upregulating H<sub>2</sub>O<sub>2</sub> content and CAT activity. Five minutes was an appropriate time to treat DBD plasma.https://www.mdpi.com/2311-7524/9/12/1269catalaseDBD plasmahydrogen peroxide<i>Ipomoea aquatica</i> Forssk.malondialdehydewettability |
| spellingShingle | Prapasiri Ongrak Nopporn Poolyarat Suebsak Suksaengpanomrung Kamtorn Saidarasamoot Yaowapha Jirakiattikul Panumart Rithichai Germination, Physicochemical Properties, and Antioxidant Enzyme Activities in Kangkong (<i>Ipomoea aquatica</i> Forssk.) Seeds as Affected by Dielectric Barrier Discharge Plasma Horticulturae catalase DBD plasma hydrogen peroxide <i>Ipomoea aquatica</i> Forssk. malondialdehyde wettability |
| title | Germination, Physicochemical Properties, and Antioxidant Enzyme Activities in Kangkong (<i>Ipomoea aquatica</i> Forssk.) Seeds as Affected by Dielectric Barrier Discharge Plasma |
| title_full | Germination, Physicochemical Properties, and Antioxidant Enzyme Activities in Kangkong (<i>Ipomoea aquatica</i> Forssk.) Seeds as Affected by Dielectric Barrier Discharge Plasma |
| title_fullStr | Germination, Physicochemical Properties, and Antioxidant Enzyme Activities in Kangkong (<i>Ipomoea aquatica</i> Forssk.) Seeds as Affected by Dielectric Barrier Discharge Plasma |
| title_full_unstemmed | Germination, Physicochemical Properties, and Antioxidant Enzyme Activities in Kangkong (<i>Ipomoea aquatica</i> Forssk.) Seeds as Affected by Dielectric Barrier Discharge Plasma |
| title_short | Germination, Physicochemical Properties, and Antioxidant Enzyme Activities in Kangkong (<i>Ipomoea aquatica</i> Forssk.) Seeds as Affected by Dielectric Barrier Discharge Plasma |
| title_sort | germination physicochemical properties and antioxidant enzyme activities in kangkong i ipomoea aquatica i forssk seeds as affected by dielectric barrier discharge plasma |
| topic | catalase DBD plasma hydrogen peroxide <i>Ipomoea aquatica</i> Forssk. malondialdehyde wettability |
| url | https://www.mdpi.com/2311-7524/9/12/1269 |
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