Allele-Specific PCR for <i>KRAS</i> Mutation Detection Using Phosphoryl Guanidine Modified Primers

Allele-Specific PCR for <i>KRAS</i> Mutation Detection Using Phosphoryl Guanidine Modified Primers

Establishing the Kirsten rat sarcoma <i>(KRAS</i>) mutational status is essential in terms of managing patients with various types of cancer. Allele-specific real-time polymerase chain reaction (AS-PCR) is a widely used method for somatic mutations detection. To improve the limited sensi...

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Bibliographic Details
Main Authors:	Alexey S. Chubarov, Igor P. Oscorbin, Maxim L. Filipenko, Alexander A. Lomzov, Dmitrii V. Pyshnyi
Format:	Article
Language:	English
Published:	MDPI AG 2020-10-01
Series:	Diagnostics
Subjects:	mutation detection <i>KRAS</i> mutations allele-specific PCR blocker PCR modified oligonucleotides phosphoryl guanidine oligonucleotide (PGO)
Online Access:	https://www.mdpi.com/2075-4418/10/11/872

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