New insights into the raw milk microbiota diversity from animals with a different genetic predisposition for feed efficiency and resilience to mastitis

Abstract The main objective of this study was to assess the microbiota diversity in milk samples collected from Holstein cows with different estimated breeding values for predicted feed efficiency, milk coagulation, resilience to mastitis, and consequently, to study its effects on milk quality. One...

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Main Authors: Armin Tarrah, Simone Callegaro, Shadi Pakroo, Raffaella Finocchiaro, Alessio Giacomini, Viviana Corich, Martino Cassandro
Format: Article
Language:English
Published: Nature Portfolio 2022-08-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-022-17418-2
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author Armin Tarrah
Simone Callegaro
Shadi Pakroo
Raffaella Finocchiaro
Alessio Giacomini
Viviana Corich
Martino Cassandro
author_facet Armin Tarrah
Simone Callegaro
Shadi Pakroo
Raffaella Finocchiaro
Alessio Giacomini
Viviana Corich
Martino Cassandro
author_sort Armin Tarrah
collection DOAJ
description Abstract The main objective of this study was to assess the microbiota diversity in milk samples collected from Holstein cows with different estimated breeding values for predicted feed efficiency, milk coagulation, resilience to mastitis, and consequently, to study its effects on milk quality. One hundred and twenty milk samples were collected in two seasons (summer and winter) from different commercial dairy farms in the Nord-east of Italy. For each trait, 20 animals divided into two groups of the high (10 cows) and the low (10 cows) were selected to study the microbiota profile using 16S rRNA metabarcoding sequencing. The alpha and beta diversity analysis revealed significant differences between the high and the low groups for feed efficiency and resilience to mastitis, while no significant difference was detected for milk coagulation. Moreover, remarkable differences among the taxa were detected between the two seasons, where the winter was more diverse than summer when applied the Chao1 index. Lastly, the linear discriminant analysis (LDA) effect size (LEfSe) indicated Aerococcus, Corynebacterium, Facklamia, and Psychrobacter taxa with more abundance in the high group of feed efficiency, whereas, in resilience to mastitis, only two genera of Mycoplana and Rhodococcus were more abundant in the low group. In addition, LEfSe analysis between the seasons showed significant differences in the abundance of Bacteroides, Lactobacillus, Corynebacterium, Escherichia, Citrobacter, Pantoea, Pseudomonas, and Stenotrophomonas. These findings indicate that the different genetic predisposition for feed efficiency and resilience to mastitis could affect the raw milk microbiota and, consequently, its quality. Moreover, we found more abundance of mastitis-associated bacteria in the milk of dairy cows with a higher feed efficiency index.
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spelling doaj.art-bcb0ad39f9984e3a998c7bfa9512568b2022-12-22T04:01:47ZengNature PortfolioScientific Reports2045-23222022-08-011211910.1038/s41598-022-17418-2New insights into the raw milk microbiota diversity from animals with a different genetic predisposition for feed efficiency and resilience to mastitisArmin Tarrah0Simone Callegaro1Shadi Pakroo2Raffaella Finocchiaro3Alessio Giacomini4Viviana Corich5Martino Cassandro6Department of Agronomy, Food, Natural resources, Animals and Environment (DAFNAE), University of PadovaDepartment of Agronomy, Food, Natural resources, Animals and Environment (DAFNAE), University of PadovaDepartment of Agronomy, Food, Natural resources, Animals and Environment (DAFNAE), University of PadovaAssociazione Nazionale Allevatori Razza Frisona, Bruna e Jersey Italiana—ANAFIBJDepartment of Agronomy, Food, Natural resources, Animals and Environment (DAFNAE), University of PadovaDepartment of Agronomy, Food, Natural resources, Animals and Environment (DAFNAE), University of PadovaDepartment of Agronomy, Food, Natural resources, Animals and Environment (DAFNAE), University of PadovaAbstract The main objective of this study was to assess the microbiota diversity in milk samples collected from Holstein cows with different estimated breeding values for predicted feed efficiency, milk coagulation, resilience to mastitis, and consequently, to study its effects on milk quality. One hundred and twenty milk samples were collected in two seasons (summer and winter) from different commercial dairy farms in the Nord-east of Italy. For each trait, 20 animals divided into two groups of the high (10 cows) and the low (10 cows) were selected to study the microbiota profile using 16S rRNA metabarcoding sequencing. The alpha and beta diversity analysis revealed significant differences between the high and the low groups for feed efficiency and resilience to mastitis, while no significant difference was detected for milk coagulation. Moreover, remarkable differences among the taxa were detected between the two seasons, where the winter was more diverse than summer when applied the Chao1 index. Lastly, the linear discriminant analysis (LDA) effect size (LEfSe) indicated Aerococcus, Corynebacterium, Facklamia, and Psychrobacter taxa with more abundance in the high group of feed efficiency, whereas, in resilience to mastitis, only two genera of Mycoplana and Rhodococcus were more abundant in the low group. In addition, LEfSe analysis between the seasons showed significant differences in the abundance of Bacteroides, Lactobacillus, Corynebacterium, Escherichia, Citrobacter, Pantoea, Pseudomonas, and Stenotrophomonas. These findings indicate that the different genetic predisposition for feed efficiency and resilience to mastitis could affect the raw milk microbiota and, consequently, its quality. Moreover, we found more abundance of mastitis-associated bacteria in the milk of dairy cows with a higher feed efficiency index.https://doi.org/10.1038/s41598-022-17418-2
spellingShingle Armin Tarrah
Simone Callegaro
Shadi Pakroo
Raffaella Finocchiaro
Alessio Giacomini
Viviana Corich
Martino Cassandro
New insights into the raw milk microbiota diversity from animals with a different genetic predisposition for feed efficiency and resilience to mastitis
Scientific Reports
title New insights into the raw milk microbiota diversity from animals with a different genetic predisposition for feed efficiency and resilience to mastitis
title_full New insights into the raw milk microbiota diversity from animals with a different genetic predisposition for feed efficiency and resilience to mastitis
title_fullStr New insights into the raw milk microbiota diversity from animals with a different genetic predisposition for feed efficiency and resilience to mastitis
title_full_unstemmed New insights into the raw milk microbiota diversity from animals with a different genetic predisposition for feed efficiency and resilience to mastitis
title_short New insights into the raw milk microbiota diversity from animals with a different genetic predisposition for feed efficiency and resilience to mastitis
title_sort new insights into the raw milk microbiota diversity from animals with a different genetic predisposition for feed efficiency and resilience to mastitis
url https://doi.org/10.1038/s41598-022-17418-2
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