Visualised genotyping assay with oral swabs in a closed tube by nested invasive reaction assisted with gold nanoparticle probes
Abstract Single nucleotide polymorphism (SNP) typing is crucial for drug dosage and disease progression. Therefore, a simple and convenient genotyping assay is essential for personalised medicine. Herein, we developed a non‐invasive, closed‐tube, and visualised method for genotyping. In this method,...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hindawi-IET
2023-05-01
|
| Series: | IET Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1049/nbt2.12123 |
| _version_ | 1797426945307181056 |
|---|---|
| author | Yijun Li Wei Wei Yi Ma Jingwen Shan Yanan Chu LiKun Zhang Danni Liu Xueping Ma Guohua Zhou Haiping Wu |
| author_facet | Yijun Li Wei Wei Yi Ma Jingwen Shan Yanan Chu LiKun Zhang Danni Liu Xueping Ma Guohua Zhou Haiping Wu |
| author_sort | Yijun Li |
| collection | DOAJ |
| description | Abstract Single nucleotide polymorphism (SNP) typing is crucial for drug dosage and disease progression. Therefore, a simple and convenient genotyping assay is essential for personalised medicine. Herein, we developed a non‐invasive, closed‐tube, and visualised method for genotyping. In this method, oral swabs were lysed to directly perform PCR coupled with nested invasive reaction and visualisation based on gold nanoparticle probes in a closed tube. The strategy for genotyping assay depends on the single base recognition property of invasive reaction. This assay allowed quick and simple sample preparation and the detection of 25 copies/μL of CYP2C19*2 and 100 copies/μL of CYP2C19*3 within 90 min. Further, 20 oral swab samples for CYP2C19*2 and CYP2C19*3 were correctly typed, which agreed with pyrosequencing, indicating that this method has great potential for SNP typing in source‐limited regions to guide personalised medicine. |
| first_indexed | 2024-03-09T08:37:11Z |
| format | Article |
| id | doaj.art-bcb47b0817934ecfb6886b0c0fc7339d |
| institution | Directory Open Access Journal |
| issn | 1751-8741 1751-875X |
| language | English |
| last_indexed | 2024-03-09T08:37:11Z |
| publishDate | 2023-05-01 |
| publisher | Hindawi-IET |
| record_format | Article |
| series | IET Nanobiotechnology |
| spelling | doaj.art-bcb47b0817934ecfb6886b0c0fc7339d2023-12-02T17:58:29ZengHindawi-IETIET Nanobiotechnology1751-87411751-875X2023-05-0117328128810.1049/nbt2.12123Visualised genotyping assay with oral swabs in a closed tube by nested invasive reaction assisted with gold nanoparticle probesYijun Li0Wei Wei1Yi Ma2Jingwen Shan3Yanan Chu4LiKun Zhang5Danni Liu6Xueping Ma7Guohua Zhou8Haiping Wu9Department of Clinical Pharmacy Jinling Hospital School of Pharmaceutical Sciences Southern Medical University Guangzhou ChinaDepartment of Clinical Pharmacy Jinling Hospital School of Pharmaceutical Sciences Southern Medical University Guangzhou ChinaState Key Laboratory of Analytical Chemistry for Life Science and Jiangsu Key Laboratory of Molecular Medicine Medical School of Nanjing University Nanjing ChinaDepartment of Clinical Pharmacy Jinling Hospital School of Pharmaceutical Sciences Southern Medical University Guangzhou ChinaDepartment of Clinical Pharmacy Jinling Hospital School of Pharmaceutical Sciences Southern Medical University Guangzhou ChinaState Key Laboratory of Analytical Chemistry for Life Science and Jiangsu Key Laboratory of Molecular Medicine Medical School of Nanjing University Nanjing ChinaSchool of Life Science and Technology China Pharmaceutical University Nanjing ChinaDepartment of Clinical Pharmacy Jinling Hospital School of Pharmaceutical Sciences Southern Medical University Guangzhou ChinaDepartment of Clinical Pharmacy Jinling Hospital School of Pharmaceutical Sciences Southern Medical University Guangzhou ChinaDepartment of Clinical Pharmacy Jinling Hospital School of Pharmaceutical Sciences Southern Medical University Guangzhou ChinaAbstract Single nucleotide polymorphism (SNP) typing is crucial for drug dosage and disease progression. Therefore, a simple and convenient genotyping assay is essential for personalised medicine. Herein, we developed a non‐invasive, closed‐tube, and visualised method for genotyping. In this method, oral swabs were lysed to directly perform PCR coupled with nested invasive reaction and visualisation based on gold nanoparticle probes in a closed tube. The strategy for genotyping assay depends on the single base recognition property of invasive reaction. This assay allowed quick and simple sample preparation and the detection of 25 copies/μL of CYP2C19*2 and 100 copies/μL of CYP2C19*3 within 90 min. Further, 20 oral swab samples for CYP2C19*2 and CYP2C19*3 were correctly typed, which agreed with pyrosequencing, indicating that this method has great potential for SNP typing in source‐limited regions to guide personalised medicine.https://doi.org/10.1049/nbt2.12123nanobiotechnologynanoparticlespatient treatment |
| spellingShingle | Yijun Li Wei Wei Yi Ma Jingwen Shan Yanan Chu LiKun Zhang Danni Liu Xueping Ma Guohua Zhou Haiping Wu Visualised genotyping assay with oral swabs in a closed tube by nested invasive reaction assisted with gold nanoparticle probes IET Nanobiotechnology nanobiotechnology nanoparticles patient treatment |
| title | Visualised genotyping assay with oral swabs in a closed tube by nested invasive reaction assisted with gold nanoparticle probes |
| title_full | Visualised genotyping assay with oral swabs in a closed tube by nested invasive reaction assisted with gold nanoparticle probes |
| title_fullStr | Visualised genotyping assay with oral swabs in a closed tube by nested invasive reaction assisted with gold nanoparticle probes |
| title_full_unstemmed | Visualised genotyping assay with oral swabs in a closed tube by nested invasive reaction assisted with gold nanoparticle probes |
| title_short | Visualised genotyping assay with oral swabs in a closed tube by nested invasive reaction assisted with gold nanoparticle probes |
| title_sort | visualised genotyping assay with oral swabs in a closed tube by nested invasive reaction assisted with gold nanoparticle probes |
| topic | nanobiotechnology nanoparticles patient treatment |
| url | https://doi.org/10.1049/nbt2.12123 |
| work_keys_str_mv | AT yijunli visualisedgenotypingassaywithoralswabsinaclosedtubebynestedinvasivereactionassistedwithgoldnanoparticleprobes AT weiwei visualisedgenotypingassaywithoralswabsinaclosedtubebynestedinvasivereactionassistedwithgoldnanoparticleprobes AT yima visualisedgenotypingassaywithoralswabsinaclosedtubebynestedinvasivereactionassistedwithgoldnanoparticleprobes AT jingwenshan visualisedgenotypingassaywithoralswabsinaclosedtubebynestedinvasivereactionassistedwithgoldnanoparticleprobes AT yananchu visualisedgenotypingassaywithoralswabsinaclosedtubebynestedinvasivereactionassistedwithgoldnanoparticleprobes AT likunzhang visualisedgenotypingassaywithoralswabsinaclosedtubebynestedinvasivereactionassistedwithgoldnanoparticleprobes AT danniliu visualisedgenotypingassaywithoralswabsinaclosedtubebynestedinvasivereactionassistedwithgoldnanoparticleprobes AT xuepingma visualisedgenotypingassaywithoralswabsinaclosedtubebynestedinvasivereactionassistedwithgoldnanoparticleprobes AT guohuazhou visualisedgenotypingassaywithoralswabsinaclosedtubebynestedinvasivereactionassistedwithgoldnanoparticleprobes AT haipingwu visualisedgenotypingassaywithoralswabsinaclosedtubebynestedinvasivereactionassistedwithgoldnanoparticleprobes |