Physiological and Biochemical Parameters of Field Bean (<i>Vicia faba</i> var. minor) Seeds Stored for 33 Years
Changes occurring in seeds of two field bean cultivars during long-term storage at −14 °C, +4 °C and +20 °C were examined. It was found that after 33 years of storage at low temperatures, the seeds showed a significant decline in biological quality, and the seeds stored at +20 °C completely lost the...
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MDPI AG
2023-10-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/13/10/2012 |
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| author | Dariusz J. Michalczyk Magdalena Krupka Jan Kamiński Marta Wierzbicka Sonia Floryańska Wiktor Kopeć Agnieszka I. Piotrowicz-Cieślak |
| author_facet | Dariusz J. Michalczyk Magdalena Krupka Jan Kamiński Marta Wierzbicka Sonia Floryańska Wiktor Kopeć Agnieszka I. Piotrowicz-Cieślak |
| author_sort | Dariusz J. Michalczyk |
| collection | DOAJ |
| description | Changes occurring in seeds of two field bean cultivars during long-term storage at −14 °C, +4 °C and +20 °C were examined. It was found that after 33 years of storage at low temperatures, the seeds showed a significant decline in biological quality, and the seeds stored at +20 °C completely lost their germination capacity. As the seeds aged, changes in their phenolic composition, concentrations of polyamines and activity of enzymes associated with redox homeostasis and phenolic metabolism could be easily observed. The activity of ascorbate peroxidase and phenylalanine ammonia-lyase in deteriorated seeds was higher than in high-quality seeds. The activity of guaiacol peroxidase, catalase and glutathione reductase in low-quality seeds was decreased. With superoxide dismutase, the pattern was less clear-cut and depended on both seed biological quality and cultivar. Whole seed levels of spermine and spermidine decreased in ageing seeds and were lowest in non-germinating seeds. However, the opposite pattern was observed regarding spermine and spermidine, as well as putrescine, in seed coats. The obtained results indicate that changes in the activity of redox and protective systems in seeds have a clear relationship with the seed biological quality and can be detected even at a moderate level of seed deterioration. The analyses of such changes can significantly facilitate the assessment of seed quality and can therefore be of interest for seed companies and seed banks. |
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| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-10T21:31:38Z |
| publishDate | 2023-10-01 |
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| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-bcb70f12b7424e98a37842de17e134132023-11-19T15:19:53ZengMDPI AGAgriculture2077-04722023-10-011310201210.3390/agriculture13102012Physiological and Biochemical Parameters of Field Bean (<i>Vicia faba</i> var. minor) Seeds Stored for 33 YearsDariusz J. Michalczyk0Magdalena Krupka1Jan Kamiński2Marta Wierzbicka3Sonia Floryańska4Wiktor Kopeć5Agnieszka I. Piotrowicz-Cieślak6Department of Plant Physiology, Genetics and Biotechnology, University of Warmia and Mazury, Oczapowskiego Str. 1A, 10-719 Olsztyn, PolandDepartment of Plant Physiology, Genetics and Biotechnology, University of Warmia and Mazury, Oczapowskiego Str. 1A, 10-719 Olsztyn, PolandDepartment of Plant Physiology, Genetics and Biotechnology, University of Warmia and Mazury, Oczapowskiego Str. 1A, 10-719 Olsztyn, PolandDepartment of Plant Physiology, Genetics and Biotechnology, University of Warmia and Mazury, Oczapowskiego Str. 1A, 10-719 Olsztyn, PolandDepartment of Plant Physiology, Genetics and Biotechnology, University of Warmia and Mazury, Oczapowskiego Str. 1A, 10-719 Olsztyn, PolandDepartment of Plant Physiology, Genetics and Biotechnology, University of Warmia and Mazury, Oczapowskiego Str. 1A, 10-719 Olsztyn, PolandDepartment of Plant Physiology, Genetics and Biotechnology, University of Warmia and Mazury, Oczapowskiego Str. 1A, 10-719 Olsztyn, PolandChanges occurring in seeds of two field bean cultivars during long-term storage at −14 °C, +4 °C and +20 °C were examined. It was found that after 33 years of storage at low temperatures, the seeds showed a significant decline in biological quality, and the seeds stored at +20 °C completely lost their germination capacity. As the seeds aged, changes in their phenolic composition, concentrations of polyamines and activity of enzymes associated with redox homeostasis and phenolic metabolism could be easily observed. The activity of ascorbate peroxidase and phenylalanine ammonia-lyase in deteriorated seeds was higher than in high-quality seeds. The activity of guaiacol peroxidase, catalase and glutathione reductase in low-quality seeds was decreased. With superoxide dismutase, the pattern was less clear-cut and depended on both seed biological quality and cultivar. Whole seed levels of spermine and spermidine decreased in ageing seeds and were lowest in non-germinating seeds. However, the opposite pattern was observed regarding spermine and spermidine, as well as putrescine, in seed coats. The obtained results indicate that changes in the activity of redox and protective systems in seeds have a clear relationship with the seed biological quality and can be detected even at a moderate level of seed deterioration. The analyses of such changes can significantly facilitate the assessment of seed quality and can therefore be of interest for seed companies and seed banks.https://www.mdpi.com/2077-0472/13/10/2012<i>Vicia faba</i> var. minor seedslong-term storageperoxidasesuperoxide dismutaseglutathione reductasephenylalanine ammonia-lyase |
| spellingShingle | Dariusz J. Michalczyk Magdalena Krupka Jan Kamiński Marta Wierzbicka Sonia Floryańska Wiktor Kopeć Agnieszka I. Piotrowicz-Cieślak Physiological and Biochemical Parameters of Field Bean (<i>Vicia faba</i> var. minor) Seeds Stored for 33 Years Agriculture <i>Vicia faba</i> var. minor seeds long-term storage peroxidase superoxide dismutase glutathione reductase phenylalanine ammonia-lyase |
| title | Physiological and Biochemical Parameters of Field Bean (<i>Vicia faba</i> var. minor) Seeds Stored for 33 Years |
| title_full | Physiological and Biochemical Parameters of Field Bean (<i>Vicia faba</i> var. minor) Seeds Stored for 33 Years |
| title_fullStr | Physiological and Biochemical Parameters of Field Bean (<i>Vicia faba</i> var. minor) Seeds Stored for 33 Years |
| title_full_unstemmed | Physiological and Biochemical Parameters of Field Bean (<i>Vicia faba</i> var. minor) Seeds Stored for 33 Years |
| title_short | Physiological and Biochemical Parameters of Field Bean (<i>Vicia faba</i> var. minor) Seeds Stored for 33 Years |
| title_sort | physiological and biochemical parameters of field bean i vicia faba i var minor seeds stored for 33 years |
| topic | <i>Vicia faba</i> var. minor seeds long-term storage peroxidase superoxide dismutase glutathione reductase phenylalanine ammonia-lyase |
| url | https://www.mdpi.com/2077-0472/13/10/2012 |
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