blaKPC-24-Harboring Aeromonas veronii from the Hospital Sewage Samples in China
ABSTRACT KPC-24, different from KPC-2 by a single amino acid alteration at codon 6 (R6P), was initially discovered in Klebsiella pneumoniae in Chile. Here, we reported KPC-24-producing Aeromonas veronii isolates from hospital sewage in China. The blaKPC-24 was cloned and the MICs were tested against...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2022-06-01
|
| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.00555-22 |
| _version_ | 1828315020981698560 |
|---|---|
| author | Chengcheng Yang Min Guo Haizhen Yang Yicheng Wen Zhichen Zhu Tao Wang Jie Zhu Liang Chen Hong Du |
| author_facet | Chengcheng Yang Min Guo Haizhen Yang Yicheng Wen Zhichen Zhu Tao Wang Jie Zhu Liang Chen Hong Du |
| author_sort | Chengcheng Yang |
| collection | DOAJ |
| description | ABSTRACT KPC-24, different from KPC-2 by a single amino acid alteration at codon 6 (R6P), was initially discovered in Klebsiella pneumoniae in Chile. Here, we reported KPC-24-producing Aeromonas veronii isolates from hospital sewage in China. The blaKPC-24 was cloned and the MICs were tested against β-lactams antimicrobial agents. KPC-24 exhibited a β-lactam susceptibility profile similar to that of KPC-2. Whole-genome sequencing and analysis revealed that blaKPC-24 was located within a Tn6296-related region on an IncP-6 plasmid. IMPORTANCE Our study described a variant of K. pneumoniae carbapenemase (KPC), KPC-24, from two A. veronii strains isolated from hospital sewage, in which antibiotics, biocides, pharmaceuticals, and heavy metals may supply an appropriate condition for the evolution of carbapenemases. Some variants exhibited stronger hydrolysis activity to antibiotics and gave rise to a major public health concern. More seriously, Aeromonas species are prevalent in aquatic environments and, thus, may act as a suitable vector for antibiotics-resistance genes and foster the transmission of resistance. We should attach importance to surveying the evolution and transmission of antibiotics-resistance genes. |
| first_indexed | 2024-04-13T16:54:10Z |
| format | Article |
| id | doaj.art-bcba27c812a14c5887c257b262568053 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-04-13T16:54:10Z |
| publishDate | 2022-06-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-bcba27c812a14c5887c257b2625680532022-12-22T02:38:52ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972022-06-0110310.1128/spectrum.00555-22blaKPC-24-Harboring Aeromonas veronii from the Hospital Sewage Samples in ChinaChengcheng Yang0Min Guo1Haizhen Yang2Yicheng Wen3Zhichen Zhu4Tao Wang5Jie Zhu6Liang Chen7Hong Du8Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, People’s Republic of ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, People’s Republic of ChinaHealth Management Center, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, People’s Republic of ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, People’s Republic of ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, People’s Republic of ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, People’s Republic of ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, People’s Republic of ChinaHackensack Meridian Health Center for Discovery and Innovation, Nutley, New Jersey, USADepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, People’s Republic of ChinaABSTRACT KPC-24, different from KPC-2 by a single amino acid alteration at codon 6 (R6P), was initially discovered in Klebsiella pneumoniae in Chile. Here, we reported KPC-24-producing Aeromonas veronii isolates from hospital sewage in China. The blaKPC-24 was cloned and the MICs were tested against β-lactams antimicrobial agents. KPC-24 exhibited a β-lactam susceptibility profile similar to that of KPC-2. Whole-genome sequencing and analysis revealed that blaKPC-24 was located within a Tn6296-related region on an IncP-6 plasmid. IMPORTANCE Our study described a variant of K. pneumoniae carbapenemase (KPC), KPC-24, from two A. veronii strains isolated from hospital sewage, in which antibiotics, biocides, pharmaceuticals, and heavy metals may supply an appropriate condition for the evolution of carbapenemases. Some variants exhibited stronger hydrolysis activity to antibiotics and gave rise to a major public health concern. More seriously, Aeromonas species are prevalent in aquatic environments and, thus, may act as a suitable vector for antibiotics-resistance genes and foster the transmission of resistance. We should attach importance to surveying the evolution and transmission of antibiotics-resistance genes.https://journals.asm.org/doi/10.1128/spectrum.00555-22Aeromonas veroniiIncP-6 plasmidKPC-24carbapenemase variantshospital sewage |
| spellingShingle | Chengcheng Yang Min Guo Haizhen Yang Yicheng Wen Zhichen Zhu Tao Wang Jie Zhu Liang Chen Hong Du blaKPC-24-Harboring Aeromonas veronii from the Hospital Sewage Samples in China Microbiology Spectrum Aeromonas veronii IncP-6 plasmid KPC-24 carbapenemase variants hospital sewage |
| title | blaKPC-24-Harboring Aeromonas veronii from the Hospital Sewage Samples in China |
| title_full | blaKPC-24-Harboring Aeromonas veronii from the Hospital Sewage Samples in China |
| title_fullStr | blaKPC-24-Harboring Aeromonas veronii from the Hospital Sewage Samples in China |
| title_full_unstemmed | blaKPC-24-Harboring Aeromonas veronii from the Hospital Sewage Samples in China |
| title_short | blaKPC-24-Harboring Aeromonas veronii from the Hospital Sewage Samples in China |
| title_sort | blakpc 24 harboring aeromonas veronii from the hospital sewage samples in china |
| topic | Aeromonas veronii IncP-6 plasmid KPC-24 carbapenemase variants hospital sewage |
| url | https://journals.asm.org/doi/10.1128/spectrum.00555-22 |
| work_keys_str_mv | AT chengchengyang blakpc24harboringaeromonasveroniifromthehospitalsewagesamplesinchina AT minguo blakpc24harboringaeromonasveroniifromthehospitalsewagesamplesinchina AT haizhenyang blakpc24harboringaeromonasveroniifromthehospitalsewagesamplesinchina AT yichengwen blakpc24harboringaeromonasveroniifromthehospitalsewagesamplesinchina AT zhichenzhu blakpc24harboringaeromonasveroniifromthehospitalsewagesamplesinchina AT taowang blakpc24harboringaeromonasveroniifromthehospitalsewagesamplesinchina AT jiezhu blakpc24harboringaeromonasveroniifromthehospitalsewagesamplesinchina AT liangchen blakpc24harboringaeromonasveroniifromthehospitalsewagesamplesinchina AT hongdu blakpc24harboringaeromonasveroniifromthehospitalsewagesamplesinchina |