Host‐Microbiota Communication in Spontaneously Hypertensive Rats Generates Unique IgA‐Coated Gut Microbes
Background Hypertension is associated with gut dysbiosis, altered intestinal immunity, and gut pathology in animal models and humans. Although these findings have implicated impaired interactions between gut and gut microbiota in hypertension, little is known about the specific functional gut microb...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-02-01
|
| Series: | Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease |
| Subjects: | |
| Online Access: | https://www.ahajournals.org/doi/10.1161/JAHA.122.027918 |
| _version_ | 1797606801817993216 |
|---|---|
| author | Jing Li Elaine M. Richards Ramakumar Tummala Carl J. Pepine Mohan K. Raizada Tao Yang |
| author_facet | Jing Li Elaine M. Richards Ramakumar Tummala Carl J. Pepine Mohan K. Raizada Tao Yang |
| author_sort | Jing Li |
| collection | DOAJ |
| description | Background Hypertension is associated with gut dysbiosis, altered intestinal immunity, and gut pathology in animal models and humans. Although these findings have implicated impaired interactions between gut and gut microbiota in hypertension, little is known about the specific functional gut microbes that interact with intestinal mucosa. Methods and Results To identify these microbes, we sorted Immunoglobin A (IgA)‐coated (IgA+) and IgA‐noncoated (IgA−) bacteria using a combination of magnetic‐activated cell sorting and fluorescence‐activated cell sorting, and subsequently performed 16 S rRNA gene sequencing (IgA‐SEQ) to determine the microbial composition of IgA+ and IgA− fractions in spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto rats. We observed a significant decrease in IgA+ bacteria in SHR compared with Wistar Kyoto and a distinct composition of IgA+ and IgA− bacteria between Wistar Kyoto and SHR, showing more IgA‐bound Proteobacteria, Bacteroidetes and Actinobacteria but less of Firmicutes in SHR at the phylum level. We further identified enriched IgA‐coated Romboutsia, Turicibacter, Ileibacterium, and Dubosiella in SHR that were negatively correlated with the various pathways including antigen presentation, immune response, cell junction organization, epithelium development, and defense response to virus. Conclusions We demonstrate new IgA‐coated bacteria that participate in host‐microbiota communication in hypertension, suggesting promising therapeutic interventions targeting these bacteria for hypertension management. |
| first_indexed | 2024-03-11T05:21:14Z |
| format | Article |
| id | doaj.art-bcbf225d3c214aa394a2bfea55983581 |
| institution | Directory Open Access Journal |
| issn | 2047-9980 |
| language | English |
| last_indexed | 2024-03-11T05:21:14Z |
| publishDate | 2023-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease |
| spelling | doaj.art-bcbf225d3c214aa394a2bfea559835812023-11-17T17:54:26ZengWileyJournal of the American Heart Association: Cardiovascular and Cerebrovascular Disease2047-99802023-02-0112410.1161/JAHA.122.027918Host‐Microbiota Communication in Spontaneously Hypertensive Rats Generates Unique IgA‐Coated Gut MicrobesJing Li0Elaine M. Richards1Ramakumar Tummala2Carl J. Pepine3Mohan K. Raizada4Tao Yang5Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden Chinese Academy of Sciences Wuhan Hubei ChinaDepartment of Physiology and Functional Genomics University of Florida College of Medicine Gainesville FL USADepartment of Physiology and Pharmacology Center for Hypertension and Precision Medicine College of Medicine and Life Sciences University of Toledo OH USADivision of Cardiovascular Medicine Department of Medicine University of Florida College of Medicine Gainesville FL USADepartment of Physiology and Functional Genomics University of Florida College of Medicine Gainesville FL USADepartment of Physiology and Pharmacology Center for Hypertension and Precision Medicine College of Medicine and Life Sciences University of Toledo OH USABackground Hypertension is associated with gut dysbiosis, altered intestinal immunity, and gut pathology in animal models and humans. Although these findings have implicated impaired interactions between gut and gut microbiota in hypertension, little is known about the specific functional gut microbes that interact with intestinal mucosa. Methods and Results To identify these microbes, we sorted Immunoglobin A (IgA)‐coated (IgA+) and IgA‐noncoated (IgA−) bacteria using a combination of magnetic‐activated cell sorting and fluorescence‐activated cell sorting, and subsequently performed 16 S rRNA gene sequencing (IgA‐SEQ) to determine the microbial composition of IgA+ and IgA− fractions in spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto rats. We observed a significant decrease in IgA+ bacteria in SHR compared with Wistar Kyoto and a distinct composition of IgA+ and IgA− bacteria between Wistar Kyoto and SHR, showing more IgA‐bound Proteobacteria, Bacteroidetes and Actinobacteria but less of Firmicutes in SHR at the phylum level. We further identified enriched IgA‐coated Romboutsia, Turicibacter, Ileibacterium, and Dubosiella in SHR that were negatively correlated with the various pathways including antigen presentation, immune response, cell junction organization, epithelium development, and defense response to virus. Conclusions We demonstrate new IgA‐coated bacteria that participate in host‐microbiota communication in hypertension, suggesting promising therapeutic interventions targeting these bacteria for hypertension management.https://www.ahajournals.org/doi/10.1161/JAHA.122.027918host‐microbiota communicationhypertensionImmunoglobin A‐coated bacteria |
| spellingShingle | Jing Li Elaine M. Richards Ramakumar Tummala Carl J. Pepine Mohan K. Raizada Tao Yang Host‐Microbiota Communication in Spontaneously Hypertensive Rats Generates Unique IgA‐Coated Gut Microbes Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease host‐microbiota communication hypertension Immunoglobin A‐coated bacteria |
| title | Host‐Microbiota Communication in Spontaneously Hypertensive Rats Generates Unique IgA‐Coated Gut Microbes |
| title_full | Host‐Microbiota Communication in Spontaneously Hypertensive Rats Generates Unique IgA‐Coated Gut Microbes |
| title_fullStr | Host‐Microbiota Communication in Spontaneously Hypertensive Rats Generates Unique IgA‐Coated Gut Microbes |
| title_full_unstemmed | Host‐Microbiota Communication in Spontaneously Hypertensive Rats Generates Unique IgA‐Coated Gut Microbes |
| title_short | Host‐Microbiota Communication in Spontaneously Hypertensive Rats Generates Unique IgA‐Coated Gut Microbes |
| title_sort | host microbiota communication in spontaneously hypertensive rats generates unique iga coated gut microbes |
| topic | host‐microbiota communication hypertension Immunoglobin A‐coated bacteria |
| url | https://www.ahajournals.org/doi/10.1161/JAHA.122.027918 |
| work_keys_str_mv | AT jingli hostmicrobiotacommunicationinspontaneouslyhypertensiveratsgeneratesuniqueigacoatedgutmicrobes AT elainemrichards hostmicrobiotacommunicationinspontaneouslyhypertensiveratsgeneratesuniqueigacoatedgutmicrobes AT ramakumartummala hostmicrobiotacommunicationinspontaneouslyhypertensiveratsgeneratesuniqueigacoatedgutmicrobes AT carljpepine hostmicrobiotacommunicationinspontaneouslyhypertensiveratsgeneratesuniqueigacoatedgutmicrobes AT mohankraizada hostmicrobiotacommunicationinspontaneouslyhypertensiveratsgeneratesuniqueigacoatedgutmicrobes AT taoyang hostmicrobiotacommunicationinspontaneouslyhypertensiveratsgeneratesuniqueigacoatedgutmicrobes |