Induction of spontaneous curvature and endocytosis: Unwanted consequences of cholesterol extraction using methyl-β-Cyclodextrin
Membrane curvature is a property of biological membranes essential for organelle morphology and the formation of tubulovesicular carriers. Curvature generation is influenced by the lipid composition of the membrane and protein-mediated processes. Lipids with small headgroups, such as phosphatidic ac...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-03-01
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| Series: | Communicative & Integrative Biology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/19420889.2018.1444306 |
| _version_ | 1818578899833978880 |
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| author | Takashi Hirama Gregory D. Fairn |
| author_facet | Takashi Hirama Gregory D. Fairn |
| author_sort | Takashi Hirama |
| collection | DOAJ |
| description | Membrane curvature is a property of biological membranes essential for organelle morphology and the formation of tubulovesicular carriers. Curvature generation is influenced by the lipid composition of the membrane and protein-mediated processes. Lipids with small headgroups, such as phosphatidic acid, are conical and impose negative curvature on a monolayer. Conversely, lipids with large headgroups relative to the hydrophobic tail(s), such as lysophosphatidylcholine, have an inverted conical shape and impose positive curvature. Due to its abundance and high rates of spontaneous flip-flop between membrane leaflets cholesterol is proposed to buffer the formation of membrane curvature. Recently, we demonstrated that cholesterol is also crucial for maintaining the proper spacing of anionic phospholipids. Upon extraction of cholesterol with cyclodextrin there is a sharp increase in the negative surface charge density of the plasma membrane, which promotes electrostatic repulsion between anionic headgroups, the generation of spontaneous positive curvature and rapid membrane internalization. |
| first_indexed | 2024-12-16T06:53:09Z |
| format | Article |
| id | doaj.art-bcc3a0a0039b46a493cca6dfb4dc9d7c |
| institution | Directory Open Access Journal |
| issn | 1942-0889 |
| language | English |
| last_indexed | 2024-12-16T06:53:09Z |
| publishDate | 2018-03-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Communicative & Integrative Biology |
| spelling | doaj.art-bcc3a0a0039b46a493cca6dfb4dc9d7c2022-12-21T22:40:21ZengTaylor & Francis GroupCommunicative & Integrative Biology1942-08892018-03-011121410.1080/19420889.2018.14443061444306Induction of spontaneous curvature and endocytosis: Unwanted consequences of cholesterol extraction using methyl-β-CyclodextrinTakashi Hirama0Gregory D. Fairn1University of TorontoKeenan Research Centre for Biomedical Science, St. Michael's HospitalMembrane curvature is a property of biological membranes essential for organelle morphology and the formation of tubulovesicular carriers. Curvature generation is influenced by the lipid composition of the membrane and protein-mediated processes. Lipids with small headgroups, such as phosphatidic acid, are conical and impose negative curvature on a monolayer. Conversely, lipids with large headgroups relative to the hydrophobic tail(s), such as lysophosphatidylcholine, have an inverted conical shape and impose positive curvature. Due to its abundance and high rates of spontaneous flip-flop between membrane leaflets cholesterol is proposed to buffer the formation of membrane curvature. Recently, we demonstrated that cholesterol is also crucial for maintaining the proper spacing of anionic phospholipids. Upon extraction of cholesterol with cyclodextrin there is a sharp increase in the negative surface charge density of the plasma membrane, which promotes electrostatic repulsion between anionic headgroups, the generation of spontaneous positive curvature and rapid membrane internalization.http://dx.doi.org/10.1080/19420889.2018.1444306CholesterolCyclodextrinPhosphatidylserinePlasma membraneSpontaneous Curvature |
| spellingShingle | Takashi Hirama Gregory D. Fairn Induction of spontaneous curvature and endocytosis: Unwanted consequences of cholesterol extraction using methyl-β-Cyclodextrin Communicative & Integrative Biology Cholesterol Cyclodextrin Phosphatidylserine Plasma membrane Spontaneous Curvature |
| title | Induction of spontaneous curvature and endocytosis: Unwanted consequences of cholesterol extraction using methyl-β-Cyclodextrin |
| title_full | Induction of spontaneous curvature and endocytosis: Unwanted consequences of cholesterol extraction using methyl-β-Cyclodextrin |
| title_fullStr | Induction of spontaneous curvature and endocytosis: Unwanted consequences of cholesterol extraction using methyl-β-Cyclodextrin |
| title_full_unstemmed | Induction of spontaneous curvature and endocytosis: Unwanted consequences of cholesterol extraction using methyl-β-Cyclodextrin |
| title_short | Induction of spontaneous curvature and endocytosis: Unwanted consequences of cholesterol extraction using methyl-β-Cyclodextrin |
| title_sort | induction of spontaneous curvature and endocytosis unwanted consequences of cholesterol extraction using methyl β cyclodextrin |
| topic | Cholesterol Cyclodextrin Phosphatidylserine Plasma membrane Spontaneous Curvature |
| url | http://dx.doi.org/10.1080/19420889.2018.1444306 |
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