Identifikasi Spesies Meloidogyne pada Wortel berdasarkan Sikuen Nukleotida
Root-knot nematodes (RKN), Meloidogyne spp., were reported as the cause of carrot branched tuber on several vegetable production areas in Central and East Java. Species identification by molecular approach was conducted using infected carrot tubers from Agropolitan Cianjur, West Java. DNA was extra...
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| Format: | Article |
| Language: | Indonesian |
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Perhimpunan Fitopatologi Indonesia
2014-02-01
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| Series: | Jurnal Fitopatologi Indonesia |
| Subjects: | |
| Online Access: | https://journal.ipb.ac.id/index.php/jfiti/article/view/7635 |
| _version_ | 1797962587820785664 |
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| author | Halimah Halimah Supramana Supramana Gede Suastika |
| author_facet | Halimah Halimah Supramana Supramana Gede Suastika |
| author_sort | Halimah Halimah |
| collection | DOAJ |
| description |
Root-knot nematodes (RKN), Meloidogyne spp., were reported as the cause of carrot branched tuber on several vegetable production areas in Central and East Java. Species identification by molecular approach was conducted using infected carrot tubers from Agropolitan Cianjur, West Java. DNA was extracted from female nematodes and amplified using PCR with species specific primers (Fjav/Rjav for M. javanica, Far/Rar for M. arenaria, and Finc/Rinc for M. incognita) and multiplex primer (M. hapla, M. chitwoodi, and M. fallax). PCR product were sequenced without cloning. Based on nucleotide sequences, two species RKN were found associated with branched tuber disease of carrot in Agropolitan Cianjur, i.e M. javanica and M. hapla. Phylogenetic analysis showed that M. javanica from Cianjur was closely related to RKN from China with the homology level of 91.9%, whereas nucleotide sequence of M. hapla from Cianjur had high homology level (99.4% to 100%) with isolates from Swiss, USA, UK, and China.
Key words: Meloidogyne hapla, M. javanica, phylogenetic, root-knot nematode
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| first_indexed | 2024-04-11T01:16:37Z |
| format | Article |
| id | doaj.art-bcc4baf160f84086b7b358f1ede0085d |
| institution | Directory Open Access Journal |
| issn | 0215-7950 2339-2479 |
| language | Indonesian |
| last_indexed | 2024-04-11T01:16:37Z |
| publishDate | 2014-02-01 |
| publisher | Perhimpunan Fitopatologi Indonesia |
| record_format | Article |
| series | Jurnal Fitopatologi Indonesia |
| spelling | doaj.art-bcc4baf160f84086b7b358f1ede0085d2023-01-04T02:45:22ZindPerhimpunan Fitopatologi IndonesiaJurnal Fitopatologi Indonesia0215-79502339-24792014-02-019110.14692/jfi.9.1.16568Identifikasi Spesies Meloidogyne pada Wortel berdasarkan Sikuen NukleotidaHalimah Halimah0Supramana Supramana1Gede Suastika2Institut Pertanian BogorInstitut Pertanian BogorInstitut Pertanian Bogor Root-knot nematodes (RKN), Meloidogyne spp., were reported as the cause of carrot branched tuber on several vegetable production areas in Central and East Java. Species identification by molecular approach was conducted using infected carrot tubers from Agropolitan Cianjur, West Java. DNA was extracted from female nematodes and amplified using PCR with species specific primers (Fjav/Rjav for M. javanica, Far/Rar for M. arenaria, and Finc/Rinc for M. incognita) and multiplex primer (M. hapla, M. chitwoodi, and M. fallax). PCR product were sequenced without cloning. Based on nucleotide sequences, two species RKN were found associated with branched tuber disease of carrot in Agropolitan Cianjur, i.e M. javanica and M. hapla. Phylogenetic analysis showed that M. javanica from Cianjur was closely related to RKN from China with the homology level of 91.9%, whereas nucleotide sequence of M. hapla from Cianjur had high homology level (99.4% to 100%) with isolates from Swiss, USA, UK, and China. Key words: Meloidogyne hapla, M. javanica, phylogenetic, root-knot nematode https://journal.ipb.ac.id/index.php/jfiti/article/view/7635Meloidogyne haplaM. javanicaphylogeneticroot-knot nematode |
| spellingShingle | Halimah Halimah Supramana Supramana Gede Suastika Identifikasi Spesies Meloidogyne pada Wortel berdasarkan Sikuen Nukleotida Jurnal Fitopatologi Indonesia Meloidogyne hapla M. javanica phylogenetic root-knot nematode |
| title | Identifikasi Spesies Meloidogyne pada Wortel berdasarkan Sikuen Nukleotida |
| title_full | Identifikasi Spesies Meloidogyne pada Wortel berdasarkan Sikuen Nukleotida |
| title_fullStr | Identifikasi Spesies Meloidogyne pada Wortel berdasarkan Sikuen Nukleotida |
| title_full_unstemmed | Identifikasi Spesies Meloidogyne pada Wortel berdasarkan Sikuen Nukleotida |
| title_short | Identifikasi Spesies Meloidogyne pada Wortel berdasarkan Sikuen Nukleotida |
| title_sort | identifikasi spesies meloidogyne pada wortel berdasarkan sikuen nukleotida |
| topic | Meloidogyne hapla M. javanica phylogenetic root-knot nematode |
| url | https://journal.ipb.ac.id/index.php/jfiti/article/view/7635 |
| work_keys_str_mv | AT halimahhalimah identifikasispesiesmeloidogynepadawortelberdasarkansikuennukleotida AT supramanasupramana identifikasispesiesmeloidogynepadawortelberdasarkansikuennukleotida AT gedesuastika identifikasispesiesmeloidogynepadawortelberdasarkansikuennukleotida |