Interactions and Dissociation Constants of Galactomannan Rendered Cellulose Films with Concavalin A by SPR Spectroscopy
Interactions of biomolecules at interfaces are important for a variety of physiological processes. Among these, interactions of lectins with monosaccharides have been investigated extensively in the past, while polysaccharide-lectin interactions have scarcely been investigated. Here, we explore the...
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MDPI AG
2020-12-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/12/12/3040 |
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| author | Pilar Vilaró Carina Sampl Gundula Teichert Werner Schlemmer Mathias Hobisch Michael Weissl Luis Panizzolo Fernando Ferreira Stefan Spirk |
| author_facet | Pilar Vilaró Carina Sampl Gundula Teichert Werner Schlemmer Mathias Hobisch Michael Weissl Luis Panizzolo Fernando Ferreira Stefan Spirk |
| author_sort | Pilar Vilaró |
| collection | DOAJ |
| description | Interactions of biomolecules at interfaces are important for a variety of physiological processes. Among these, interactions of lectins with monosaccharides have been investigated extensively in the past, while polysaccharide-lectin interactions have scarcely been investigated. Here, we explore the adsorption of galactomannans (GM) extracted from <i>Prosopis affinis</i> on cellulose thin films determined by a combination of multi-parameter surface plasmon resonance spectroscopy (MP-SPR) and atomic force microscopy (AFM). The galactomannan adsorbs spontaneously on the cellulose surfaces forming monolayer type coverage (0.60 ± 0.20 mg·m<sup>−2</sup>). The interaction of a lectin, Concavalin A (ConA), with these GM rendered cellulose surfaces using MP-SPR has been investigated and the dissociation constant K<sub>D</sub> (2.1 ± 0.8 × 10<sup>−8</sup> M) was determined in a range from 3.4 to 27.3 nM. The experiments revealed that the galactose side chains as well as the mannose reducing end of the GM are weakly interacting with the active sites of the lectins, whereas these interactions are potentially amplified by hydrophobic effects between the non-ionic GM and the lectins, thereby leading to an irreversible adsorption. |
| first_indexed | 2024-03-10T13:57:26Z |
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| id | doaj.art-bcc7677745d14a5283b76db5dd71588e |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T13:57:26Z |
| publishDate | 2020-12-01 |
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| series | Polymers |
| spelling | doaj.art-bcc7677745d14a5283b76db5dd71588e2023-11-21T01:32:28ZengMDPI AGPolymers2073-43602020-12-011212304010.3390/polym12123040Interactions and Dissociation Constants of Galactomannan Rendered Cellulose Films with Concavalin A by SPR SpectroscopyPilar Vilaró0Carina Sampl1Gundula Teichert2Werner Schlemmer3Mathias Hobisch4Michael Weissl5Luis Panizzolo6Fernando Ferreira7Stefan Spirk8Sede Tacuarembó, Espacio de Ciencia y Tecnología Química, Universidad de la República, CENUR Nores-te. Ruta 5 Km 386, Tacuarembó 45000, UruguayInstitute of Bioproducts and Paper Technology, Graz University of Technology, Inffeldgasse 23, 8010 Graz, AustriaInstitute of Bioproducts and Paper Technology, Graz University of Technology, Inffeldgasse 23, 8010 Graz, AustriaInstitute of Bioproducts and Paper Technology, Graz University of Technology, Inffeldgasse 23, 8010 Graz, AustriaInstitute of Bioproducts and Paper Technology, Graz University of Technology, Inffeldgasse 23, 8010 Graz, AustriaInstitute of Bioproducts and Paper Technology, Graz University of Technology, Inffeldgasse 23, 8010 Graz, AustriaDepartamento de Ciencia y Tecnología de Alimentos, Facultad de Química, Universidad de la República, Avenida General Flores 2124, Montevideo 11800, UruguaySede Tacuarembó, Espacio de Ciencia y Tecnología Química, Universidad de la República, CENUR Nores-te. Ruta 5 Km 386, Tacuarembó 45000, UruguayInstitute of Bioproducts and Paper Technology, Graz University of Technology, Inffeldgasse 23, 8010 Graz, AustriaInteractions of biomolecules at interfaces are important for a variety of physiological processes. Among these, interactions of lectins with monosaccharides have been investigated extensively in the past, while polysaccharide-lectin interactions have scarcely been investigated. Here, we explore the adsorption of galactomannans (GM) extracted from <i>Prosopis affinis</i> on cellulose thin films determined by a combination of multi-parameter surface plasmon resonance spectroscopy (MP-SPR) and atomic force microscopy (AFM). The galactomannan adsorbs spontaneously on the cellulose surfaces forming monolayer type coverage (0.60 ± 0.20 mg·m<sup>−2</sup>). The interaction of a lectin, Concavalin A (ConA), with these GM rendered cellulose surfaces using MP-SPR has been investigated and the dissociation constant K<sub>D</sub> (2.1 ± 0.8 × 10<sup>−8</sup> M) was determined in a range from 3.4 to 27.3 nM. The experiments revealed that the galactose side chains as well as the mannose reducing end of the GM are weakly interacting with the active sites of the lectins, whereas these interactions are potentially amplified by hydrophobic effects between the non-ionic GM and the lectins, thereby leading to an irreversible adsorption.https://www.mdpi.com/2073-4360/12/12/3040galactomannancellulose thin filmslectins |
| spellingShingle | Pilar Vilaró Carina Sampl Gundula Teichert Werner Schlemmer Mathias Hobisch Michael Weissl Luis Panizzolo Fernando Ferreira Stefan Spirk Interactions and Dissociation Constants of Galactomannan Rendered Cellulose Films with Concavalin A by SPR Spectroscopy Polymers galactomannan cellulose thin films lectins |
| title | Interactions and Dissociation Constants of Galactomannan Rendered Cellulose Films with Concavalin A by SPR Spectroscopy |
| title_full | Interactions and Dissociation Constants of Galactomannan Rendered Cellulose Films with Concavalin A by SPR Spectroscopy |
| title_fullStr | Interactions and Dissociation Constants of Galactomannan Rendered Cellulose Films with Concavalin A by SPR Spectroscopy |
| title_full_unstemmed | Interactions and Dissociation Constants of Galactomannan Rendered Cellulose Films with Concavalin A by SPR Spectroscopy |
| title_short | Interactions and Dissociation Constants of Galactomannan Rendered Cellulose Films with Concavalin A by SPR Spectroscopy |
| title_sort | interactions and dissociation constants of galactomannan rendered cellulose films with concavalin a by spr spectroscopy |
| topic | galactomannan cellulose thin films lectins |
| url | https://www.mdpi.com/2073-4360/12/12/3040 |
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