The Effect of Temperature on the Hypersensitive Response (HR) in the <i>Brassica napus–Leptosphaeria maculans</i> Pathosystem
Temperature is considered one of the crucial environmental elements in plant pathological interactions, and previous studies have indicated that there is a relationship between temperature change and host–pathogen interactions. The objective of this research is to investigate the link between temper...
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MDPI AG
2021-04-01
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| author | Cunchun Yang Zhongwei Zou Wannakuwattewaduge Gerard Dilantha Fernando |
| author_facet | Cunchun Yang Zhongwei Zou Wannakuwattewaduge Gerard Dilantha Fernando |
| author_sort | Cunchun Yang |
| collection | DOAJ |
| description | Temperature is considered one of the crucial environmental elements in plant pathological interactions, and previous studies have indicated that there is a relationship between temperature change and host–pathogen interactions. The objective of this research is to investigate the link between temperature and the incompatible interactions of the host and pathogen. In this study, two <i>Leptosphaeria maculans</i> isolates (HCRT75 8-1 and HCRT77 7-2) and two <i>Brassica napus</i> genotypes (Surpass400 and 01-23-2-1) were selected. The selected <i>B. napus</i> genotypes displayed intermediate and resistant phenotypes. The inoculated seedlings were tested under three temperature conditions: 16 °C/10 °C, 22 °C/16 °C and 28 °C/22 °C (day/night: 16 h/8 h). Lesion measurements demonstrated that the necrotic lesions from the 28 °C/22 °C treatment were enlarged compared with the other two temperature treatments (i.e., 16 °C/10 °C and 22 °C/16 °C). The results of expression analysis indicated that the three temperature treatments displayed distinct differences in two marker genes (<i>PATHOGENESIS–RELATED (PR) 1</i> and <i>2</i>) for plant defense and one temperature-sensitive gene <i>BONZAI 1</i> (<i>BON1</i>). Additionally, seven dpi at 22 °C/16 °C appeared to be the optimal pre-condition for the induction of <i>PR1</i> and <i>2</i>. These findings suggest that <i>B. napus</i> responds to temperature changes when infected with <i>L. maculans</i>. |
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| spelling | doaj.art-bcc7857f983f4f22866290c0c8a357db2023-11-21T16:38:07ZengMDPI AGPlants2223-77472021-04-0110584310.3390/plants10050843The Effect of Temperature on the Hypersensitive Response (HR) in the <i>Brassica napus–Leptosphaeria maculans</i> PathosystemCunchun Yang0Zhongwei Zou1Wannakuwattewaduge Gerard Dilantha Fernando2Department of Plant Science, Faculty of Agriculture and Food Science, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaDepartment of Plant Science, Faculty of Agriculture and Food Science, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaDepartment of Plant Science, Faculty of Agriculture and Food Science, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaTemperature is considered one of the crucial environmental elements in plant pathological interactions, and previous studies have indicated that there is a relationship between temperature change and host–pathogen interactions. The objective of this research is to investigate the link between temperature and the incompatible interactions of the host and pathogen. In this study, two <i>Leptosphaeria maculans</i> isolates (HCRT75 8-1 and HCRT77 7-2) and two <i>Brassica napus</i> genotypes (Surpass400 and 01-23-2-1) were selected. The selected <i>B. napus</i> genotypes displayed intermediate and resistant phenotypes. The inoculated seedlings were tested under three temperature conditions: 16 °C/10 °C, 22 °C/16 °C and 28 °C/22 °C (day/night: 16 h/8 h). Lesion measurements demonstrated that the necrotic lesions from the 28 °C/22 °C treatment were enlarged compared with the other two temperature treatments (i.e., 16 °C/10 °C and 22 °C/16 °C). The results of expression analysis indicated that the three temperature treatments displayed distinct differences in two marker genes (<i>PATHOGENESIS–RELATED (PR) 1</i> and <i>2</i>) for plant defense and one temperature-sensitive gene <i>BONZAI 1</i> (<i>BON1</i>). Additionally, seven dpi at 22 °C/16 °C appeared to be the optimal pre-condition for the induction of <i>PR1</i> and <i>2</i>. These findings suggest that <i>B. napus</i> responds to temperature changes when infected with <i>L. maculans</i>.https://www.mdpi.com/2223-7747/10/5/843<i>Brassica napus</i><i>Leptosphaeria maculans</i>gene-for-gene interactiontemperature<i>BONZAI1 (BON1)</i>pathogenesis-related protein (PR) |
| spellingShingle | Cunchun Yang Zhongwei Zou Wannakuwattewaduge Gerard Dilantha Fernando The Effect of Temperature on the Hypersensitive Response (HR) in the <i>Brassica napus–Leptosphaeria maculans</i> Pathosystem Plants <i>Brassica napus</i> <i>Leptosphaeria maculans</i> gene-for-gene interaction temperature <i>BONZAI1 (BON1)</i> pathogenesis-related protein (PR) |
| title | The Effect of Temperature on the Hypersensitive Response (HR) in the <i>Brassica napus–Leptosphaeria maculans</i> Pathosystem |
| title_full | The Effect of Temperature on the Hypersensitive Response (HR) in the <i>Brassica napus–Leptosphaeria maculans</i> Pathosystem |
| title_fullStr | The Effect of Temperature on the Hypersensitive Response (HR) in the <i>Brassica napus–Leptosphaeria maculans</i> Pathosystem |
| title_full_unstemmed | The Effect of Temperature on the Hypersensitive Response (HR) in the <i>Brassica napus–Leptosphaeria maculans</i> Pathosystem |
| title_short | The Effect of Temperature on the Hypersensitive Response (HR) in the <i>Brassica napus–Leptosphaeria maculans</i> Pathosystem |
| title_sort | effect of temperature on the hypersensitive response hr in the i brassica napus leptosphaeria maculans i pathosystem |
| topic | <i>Brassica napus</i> <i>Leptosphaeria maculans</i> gene-for-gene interaction temperature <i>BONZAI1 (BON1)</i> pathogenesis-related protein (PR) |
| url | https://www.mdpi.com/2223-7747/10/5/843 |
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