Heterogeneous regulation of bacterial natural product biosynthesis via a novel transcription factor
Biological diversity arises among genetically equal subpopulations in the same environment, a phenomenon called phenotypic heterogeneity. The life cycle of the enteric bacterium Photorhabdus luminescens involves a symbiotic interaction with nematodes as well as a pathogenic association with insect l...
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Format: | Article |
Language: | English |
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Elsevier
2016-11-01
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Series: | Heliyon |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S240584401630679X |
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author | Antje K. Heinrich Angela Glaeser Nicholas J. Tobias Ralf Heermann Helge B. Bode |
author_facet | Antje K. Heinrich Angela Glaeser Nicholas J. Tobias Ralf Heermann Helge B. Bode |
author_sort | Antje K. Heinrich |
collection | DOAJ |
description | Biological diversity arises among genetically equal subpopulations in the same environment, a phenomenon called phenotypic heterogeneity. The life cycle of the enteric bacterium Photorhabdus luminescens involves a symbiotic interaction with nematodes as well as a pathogenic association with insect larvae. P. luminescens exists in two distinct phenotypic forms designated as primary (1°) and secondary (2°). In contrast to 1° cells, 2° cells are non-pigmented due to the absence of natural compounds, especially anthraquinones (AQs). We identified a novel type of transcriptional regulator, AntJ, which activates expression of the antA-I operon responsible for AQ production. AntJ heterogeneously activates the AQ production in single P. luminescens 1° cells, and blocks AQ production in 2° cells. AntJ contains a proposed ligand-binding WYL-domain, which is widespread among bacteria. AntJ is one of the rare examples of regulators that mediate heterogeneous gene expression by altering activity rather than copy number in single cells. |
first_indexed | 2024-04-13T02:42:27Z |
format | Article |
id | doaj.art-bcccda213095412c9a62a7e0f9ad6eb6 |
institution | Directory Open Access Journal |
issn | 2405-8440 |
language | English |
last_indexed | 2024-04-13T02:42:27Z |
publishDate | 2016-11-01 |
publisher | Elsevier |
record_format | Article |
series | Heliyon |
spelling | doaj.art-bcccda213095412c9a62a7e0f9ad6eb62022-12-22T03:06:09ZengElsevierHeliyon2405-84402016-11-0121110.1016/j.heliyon.2016.e00197Heterogeneous regulation of bacterial natural product biosynthesis via a novel transcription factorAntje K. Heinrich0Angela Glaeser1Nicholas J. Tobias2Ralf Heermann3Helge B. Bode4Fachbereich Biowissenschaften, Merck Stiftungsprofessur für Molekulare Biotechnologie, Goethe-Universität Frankfurt, Frankfurt am Main, GermanyBereich Mikrobiologie, Biozentrum Martinsried, Ludwig-Maximilians-Universität München, München, GermanyFachbereich Biowissenschaften, Merck Stiftungsprofessur für Molekulare Biotechnologie, Goethe-Universität Frankfurt, Frankfurt am Main, GermanyBereich Mikrobiologie, Biozentrum Martinsried, Ludwig-Maximilians-Universität München, München, GermanyFachbereich Biowissenschaften, Merck Stiftungsprofessur für Molekulare Biotechnologie, Goethe-Universität Frankfurt, Frankfurt am Main, GermanyBiological diversity arises among genetically equal subpopulations in the same environment, a phenomenon called phenotypic heterogeneity. The life cycle of the enteric bacterium Photorhabdus luminescens involves a symbiotic interaction with nematodes as well as a pathogenic association with insect larvae. P. luminescens exists in two distinct phenotypic forms designated as primary (1°) and secondary (2°). In contrast to 1° cells, 2° cells are non-pigmented due to the absence of natural compounds, especially anthraquinones (AQs). We identified a novel type of transcriptional regulator, AntJ, which activates expression of the antA-I operon responsible for AQ production. AntJ heterogeneously activates the AQ production in single P. luminescens 1° cells, and blocks AQ production in 2° cells. AntJ contains a proposed ligand-binding WYL-domain, which is widespread among bacteria. AntJ is one of the rare examples of regulators that mediate heterogeneous gene expression by altering activity rather than copy number in single cells.http://www.sciencedirect.com/science/article/pii/S240584401630679XCell biologyMicrobiologyGenetics |
spellingShingle | Antje K. Heinrich Angela Glaeser Nicholas J. Tobias Ralf Heermann Helge B. Bode Heterogeneous regulation of bacterial natural product biosynthesis via a novel transcription factor Heliyon Cell biology Microbiology Genetics |
title | Heterogeneous regulation of bacterial natural product biosynthesis via a novel transcription factor |
title_full | Heterogeneous regulation of bacterial natural product biosynthesis via a novel transcription factor |
title_fullStr | Heterogeneous regulation of bacterial natural product biosynthesis via a novel transcription factor |
title_full_unstemmed | Heterogeneous regulation of bacterial natural product biosynthesis via a novel transcription factor |
title_short | Heterogeneous regulation of bacterial natural product biosynthesis via a novel transcription factor |
title_sort | heterogeneous regulation of bacterial natural product biosynthesis via a novel transcription factor |
topic | Cell biology Microbiology Genetics |
url | http://www.sciencedirect.com/science/article/pii/S240584401630679X |
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