Heterogeneous regulation of bacterial natural product biosynthesis via a novel transcription factor
Biological diversity arises among genetically equal subpopulations in the same environment, a phenomenon called phenotypic heterogeneity. The life cycle of the enteric bacterium Photorhabdus luminescens involves a symbiotic interaction with nematodes as well as a pathogenic association with insect l...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-11-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584401630679X |
| _version_ | 1811285330576277504 |
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| author | Antje K. Heinrich Angela Glaeser Nicholas J. Tobias Ralf Heermann Helge B. Bode |
| author_facet | Antje K. Heinrich Angela Glaeser Nicholas J. Tobias Ralf Heermann Helge B. Bode |
| author_sort | Antje K. Heinrich |
| collection | DOAJ |
| description | Biological diversity arises among genetically equal subpopulations in the same environment, a phenomenon called phenotypic heterogeneity. The life cycle of the enteric bacterium Photorhabdus luminescens involves a symbiotic interaction with nematodes as well as a pathogenic association with insect larvae. P. luminescens exists in two distinct phenotypic forms designated as primary (1°) and secondary (2°). In contrast to 1° cells, 2° cells are non-pigmented due to the absence of natural compounds, especially anthraquinones (AQs). We identified a novel type of transcriptional regulator, AntJ, which activates expression of the antA-I operon responsible for AQ production. AntJ heterogeneously activates the AQ production in single P. luminescens 1° cells, and blocks AQ production in 2° cells. AntJ contains a proposed ligand-binding WYL-domain, which is widespread among bacteria. AntJ is one of the rare examples of regulators that mediate heterogeneous gene expression by altering activity rather than copy number in single cells. |
| first_indexed | 2024-04-13T02:42:27Z |
| format | Article |
| id | doaj.art-bcccda213095412c9a62a7e0f9ad6eb6 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-13T02:42:27Z |
| publishDate | 2016-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-bcccda213095412c9a62a7e0f9ad6eb62022-12-22T03:06:09ZengElsevierHeliyon2405-84402016-11-0121110.1016/j.heliyon.2016.e00197Heterogeneous regulation of bacterial natural product biosynthesis via a novel transcription factorAntje K. Heinrich0Angela Glaeser1Nicholas J. Tobias2Ralf Heermann3Helge B. Bode4Fachbereich Biowissenschaften, Merck Stiftungsprofessur für Molekulare Biotechnologie, Goethe-Universität Frankfurt, Frankfurt am Main, GermanyBereich Mikrobiologie, Biozentrum Martinsried, Ludwig-Maximilians-Universität München, München, GermanyFachbereich Biowissenschaften, Merck Stiftungsprofessur für Molekulare Biotechnologie, Goethe-Universität Frankfurt, Frankfurt am Main, GermanyBereich Mikrobiologie, Biozentrum Martinsried, Ludwig-Maximilians-Universität München, München, GermanyFachbereich Biowissenschaften, Merck Stiftungsprofessur für Molekulare Biotechnologie, Goethe-Universität Frankfurt, Frankfurt am Main, GermanyBiological diversity arises among genetically equal subpopulations in the same environment, a phenomenon called phenotypic heterogeneity. The life cycle of the enteric bacterium Photorhabdus luminescens involves a symbiotic interaction with nematodes as well as a pathogenic association with insect larvae. P. luminescens exists in two distinct phenotypic forms designated as primary (1°) and secondary (2°). In contrast to 1° cells, 2° cells are non-pigmented due to the absence of natural compounds, especially anthraquinones (AQs). We identified a novel type of transcriptional regulator, AntJ, which activates expression of the antA-I operon responsible for AQ production. AntJ heterogeneously activates the AQ production in single P. luminescens 1° cells, and blocks AQ production in 2° cells. AntJ contains a proposed ligand-binding WYL-domain, which is widespread among bacteria. AntJ is one of the rare examples of regulators that mediate heterogeneous gene expression by altering activity rather than copy number in single cells.http://www.sciencedirect.com/science/article/pii/S240584401630679XCell biologyMicrobiologyGenetics |
| spellingShingle | Antje K. Heinrich Angela Glaeser Nicholas J. Tobias Ralf Heermann Helge B. Bode Heterogeneous regulation of bacterial natural product biosynthesis via a novel transcription factor Heliyon Cell biology Microbiology Genetics |
| title | Heterogeneous regulation of bacterial natural product biosynthesis via a novel transcription factor |
| title_full | Heterogeneous regulation of bacterial natural product biosynthesis via a novel transcription factor |
| title_fullStr | Heterogeneous regulation of bacterial natural product biosynthesis via a novel transcription factor |
| title_full_unstemmed | Heterogeneous regulation of bacterial natural product biosynthesis via a novel transcription factor |
| title_short | Heterogeneous regulation of bacterial natural product biosynthesis via a novel transcription factor |
| title_sort | heterogeneous regulation of bacterial natural product biosynthesis via a novel transcription factor |
| topic | Cell biology Microbiology Genetics |
| url | http://www.sciencedirect.com/science/article/pii/S240584401630679X |
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