Determinação de nitrito em águas utilizando extrato de flores

Aspects of visible spectrophotometry can be presented to students using simple experiments in which the color of the crude extract of Macroptilium lathyroides (L.) Urb. is bleached in the presence of nitrite ions in acidic medium. The dependence of the absorption intensity with time, the reaction co...

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Main Authors: Ramos Luiz Antônio, Cavalheiro Carla Cristina Schmitt, Cavalheiro Éder Tadeu Gomes
Format: Article
Language:English
Published: Sociedade Brasileira de Química 2006-01-01
Series:Química Nova
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000500037
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author Ramos Luiz Antônio
Cavalheiro Carla Cristina Schmitt
Cavalheiro Éder Tadeu Gomes
author_facet Ramos Luiz Antônio
Cavalheiro Carla Cristina Schmitt
Cavalheiro Éder Tadeu Gomes
author_sort Ramos Luiz Antônio
collection DOAJ
description Aspects of visible spectrophotometry can be presented to students using simple experiments in which the color of the crude extract of Macroptilium lathyroides (L.) Urb. is bleached in the presence of nitrite ions in acidic medium. The dependence of the absorption intensity with time, the reaction completeness and the Beer law can be demonstrated. Quantitative results for mineral water samples "contaminated" with nitrite ions were obtained from a method based on the Griess reaction and a procedure based on the bleaching reaction between the crude extract and NO2- ions. Both the Griess and the bleaching reactions were found to be time dependent. Recoveries of about 100 - 104% were obtained with these procedures. The use of natural dyes attracted students' interest enhancing the teaching process. Experiments performed by the teaching staff suggested that the proposed methodology can be performed in a 4 h class, with relative errors ranging from 0.19 to 1.86% in relation to the Griess method.
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spelling doaj.art-bccd01f3e5c54cf38db1fe39a38fb14a2022-12-21T23:37:44ZengSociedade Brasileira de QuímicaQuímica Nova0100-40421678-70642006-01-0129511141120Determinação de nitrito em águas utilizando extrato de floresRamos Luiz AntônioCavalheiro Carla Cristina SchmittCavalheiro Éder Tadeu GomesAspects of visible spectrophotometry can be presented to students using simple experiments in which the color of the crude extract of Macroptilium lathyroides (L.) Urb. is bleached in the presence of nitrite ions in acidic medium. The dependence of the absorption intensity with time, the reaction completeness and the Beer law can be demonstrated. Quantitative results for mineral water samples "contaminated" with nitrite ions were obtained from a method based on the Griess reaction and a procedure based on the bleaching reaction between the crude extract and NO2- ions. Both the Griess and the bleaching reactions were found to be time dependent. Recoveries of about 100 - 104% were obtained with these procedures. The use of natural dyes attracted students' interest enhancing the teaching process. Experiments performed by the teaching staff suggested that the proposed methodology can be performed in a 4 h class, with relative errors ranging from 0.19 to 1.86% in relation to the Griess method.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000500037acid-base chemistryinstrumental methodswater/water chemistry
spellingShingle Ramos Luiz Antônio
Cavalheiro Carla Cristina Schmitt
Cavalheiro Éder Tadeu Gomes
Determinação de nitrito em águas utilizando extrato de flores
Química Nova
acid-base chemistry
instrumental methods
water/water chemistry
title Determinação de nitrito em águas utilizando extrato de flores
title_full Determinação de nitrito em águas utilizando extrato de flores
title_fullStr Determinação de nitrito em águas utilizando extrato de flores
title_full_unstemmed Determinação de nitrito em águas utilizando extrato de flores
title_short Determinação de nitrito em águas utilizando extrato de flores
title_sort determinacao de nitrito em aguas utilizando extrato de flores
topic acid-base chemistry
instrumental methods
water/water chemistry
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000500037
work_keys_str_mv AT ramosluizantonio determinacaodenitritoemaguasutilizandoextratodeflores
AT cavalheirocarlacristinaschmitt determinacaodenitritoemaguasutilizandoextratodeflores
AT cavalheiroedertadeugomes determinacaodenitritoemaguasutilizandoextratodeflores