Effect of VEGF Stimulation on CD11b Receptor on Peripheral Eosinophils in Asthmatics
Asthma is a chronic, complex disease associated with heterogeneity in molecular pathways. Airway inflammation with different cell activation (e.g., eosinophils) and with hypersecretion of many cytokines (e.g., vascular endothelial growth factor—VEGF) might be relevant for asthma pathogenesis and res...
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MDPI AG
2023-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/10/8880 |
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| author | Krzysztof Gomułka Maciej Tota Kacper Brzdąk |
| author_facet | Krzysztof Gomułka Maciej Tota Kacper Brzdąk |
| author_sort | Krzysztof Gomułka |
| collection | DOAJ |
| description | Asthma is a chronic, complex disease associated with heterogeneity in molecular pathways. Airway inflammation with different cell activation (e.g., eosinophils) and with hypersecretion of many cytokines (e.g., vascular endothelial growth factor—VEGF) might be relevant for asthma pathogenesis and responsible for airway hyperresponsiveness and remodeling. The aim of our study was to reveal the expression of activation marker CD11b on peripheral eosinophils unstimulated and after VEGF in vitro stimulation in asthmatics with different degrees of airway narrowing. The study population included a total of 118 adult subjects: 78 patients with asthma (among them 39 patients with irreversible bronchoconstriction and 39 patients with reversible bronchoconstriction according to the bronchodilation test) and 40 healthy participants as a control group. CD11b expression on peripheral blood eosinophils was detected in vitro using the flow cytometric method without exogenous stimulation (negative control), after N-formyl-methionine-leucyl-phenylalanine stimulation (fMLP; positive control) and after stimulation with VEGF in two concentrations (250 ng/mL and 500 ng/mL). CD11b marker was slightly presented on unstimulated eosinophils in asthmatics and the subgroup with irreversible airway narrowing (<i>p</i> = 0.06 and <i>p</i> = 0.07, respectively). Stimulation with VEGF enhanced the activity of peripheral eosinophils and induced CD11b expression in asthmatics in comparison with a healthy control (<i>p</i> < 0.05), but it was dependent neither on the concentration of VEGF nor on the degree of airways narrowing in patients with asthma. We present our findings to draw attention to the potential role of VEGF in the eosinophil priming and CD11b-mediated signaling in patients with asthma which is currently undervalued. |
| first_indexed | 2024-03-11T03:39:55Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T03:39:55Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-bccf88753d0742aa9d9ab77706b411cf2023-11-18T01:43:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-012410888010.3390/ijms24108880Effect of VEGF Stimulation on CD11b Receptor on Peripheral Eosinophils in AsthmaticsKrzysztof Gomułka0Maciej Tota1Kacper Brzdąk2Clinical Department of Internal Medicine, Pneumology and Allergology, Wroclaw Medical University, ul. M. Curie-Skłodowskiej 66, 50-369 Wrocław, PolandStudent Scientific Group of Adult Allergology, Wroclaw Medical University, ul. M. Curie-Skłodowskiej 66, 50-369 Wrocław, PolandStudent Scientific Group of Adult Allergology, Wroclaw Medical University, ul. M. Curie-Skłodowskiej 66, 50-369 Wrocław, PolandAsthma is a chronic, complex disease associated with heterogeneity in molecular pathways. Airway inflammation with different cell activation (e.g., eosinophils) and with hypersecretion of many cytokines (e.g., vascular endothelial growth factor—VEGF) might be relevant for asthma pathogenesis and responsible for airway hyperresponsiveness and remodeling. The aim of our study was to reveal the expression of activation marker CD11b on peripheral eosinophils unstimulated and after VEGF in vitro stimulation in asthmatics with different degrees of airway narrowing. The study population included a total of 118 adult subjects: 78 patients with asthma (among them 39 patients with irreversible bronchoconstriction and 39 patients with reversible bronchoconstriction according to the bronchodilation test) and 40 healthy participants as a control group. CD11b expression on peripheral blood eosinophils was detected in vitro using the flow cytometric method without exogenous stimulation (negative control), after N-formyl-methionine-leucyl-phenylalanine stimulation (fMLP; positive control) and after stimulation with VEGF in two concentrations (250 ng/mL and 500 ng/mL). CD11b marker was slightly presented on unstimulated eosinophils in asthmatics and the subgroup with irreversible airway narrowing (<i>p</i> = 0.06 and <i>p</i> = 0.07, respectively). Stimulation with VEGF enhanced the activity of peripheral eosinophils and induced CD11b expression in asthmatics in comparison with a healthy control (<i>p</i> < 0.05), but it was dependent neither on the concentration of VEGF nor on the degree of airways narrowing in patients with asthma. We present our findings to draw attention to the potential role of VEGF in the eosinophil priming and CD11b-mediated signaling in patients with asthma which is currently undervalued.https://www.mdpi.com/1422-0067/24/10/8880asthmaCD11beosinophilsvascular endothelial growth factor |
| spellingShingle | Krzysztof Gomułka Maciej Tota Kacper Brzdąk Effect of VEGF Stimulation on CD11b Receptor on Peripheral Eosinophils in Asthmatics International Journal of Molecular Sciences asthma CD11b eosinophils vascular endothelial growth factor |
| title | Effect of VEGF Stimulation on CD11b Receptor on Peripheral Eosinophils in Asthmatics |
| title_full | Effect of VEGF Stimulation on CD11b Receptor on Peripheral Eosinophils in Asthmatics |
| title_fullStr | Effect of VEGF Stimulation on CD11b Receptor on Peripheral Eosinophils in Asthmatics |
| title_full_unstemmed | Effect of VEGF Stimulation on CD11b Receptor on Peripheral Eosinophils in Asthmatics |
| title_short | Effect of VEGF Stimulation on CD11b Receptor on Peripheral Eosinophils in Asthmatics |
| title_sort | effect of vegf stimulation on cd11b receptor on peripheral eosinophils in asthmatics |
| topic | asthma CD11b eosinophils vascular endothelial growth factor |
| url | https://www.mdpi.com/1422-0067/24/10/8880 |
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