Optimal z-axis scanning parameters for gynecologic cytology specimens
Background: The use of virtual microscopy (VM) in clinical cytology has been limited due to the inability to focus through three dimensional (3D) cell clusters with a single focal plane (2D images). Limited information exists regarding the optimal scanning parameters for 3D scanning. Aims: The purpo...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2013-01-01
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| Series: | Journal of Pathology Informatics |
| Subjects: | |
| Online Access: | http://www.jpathinformatics.org/article.asp?issn=2153-3539;year=2013;volume=4;issue=1;spage=38;epage=38;aulast=Donnelly |
| _version_ | 1818534675451215872 |
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| author | Amber D Donnelly Maheswari S Mukherjee Elizabeth R Lyden Julia A Bridge Subodh M Lele Najia Wright Mary F McGaughey Alicia M Culberson Adam J Horn Whitney R Wedel Stanley J Radio |
| author_facet | Amber D Donnelly Maheswari S Mukherjee Elizabeth R Lyden Julia A Bridge Subodh M Lele Najia Wright Mary F McGaughey Alicia M Culberson Adam J Horn Whitney R Wedel Stanley J Radio |
| author_sort | Amber D Donnelly |
| collection | DOAJ |
| description | Background: The use of virtual microscopy (VM) in clinical cytology has been limited due to the inability to focus through three dimensional (3D) cell clusters with a single focal plane (2D images). Limited information exists regarding the optimal scanning parameters for 3D scanning. Aims: The purpose of this study was to determine the optimal number of the focal plane levels and the optimal scanning interval to digitize gynecological (GYN) specimens prepared on SurePath™ glass slides while maintaining a manageable file size. Subjects and Methods: The iScanCoreo Au scanner (Ventana, AZ, USA) was used to digitize 192 SurePath™ glass slides at three focal plane levels at 1 μ interval. The digitized virtual images (VI) were annotated using BioImagene′s Image Viewer. Five participants interpreted the VI and recorded the focal plane level at which they felt confident and later interpreted the corresponding glass slide specimens using light microscopy (LM). The participants completed a survey about their experiences. Inter-rater agreement and concordance between the VI and the glass slide specimens were evaluated. Results: This study determined an overall high intra-rater diagnostic concordance between glass and VI (89-97%), however, the inter-rater agreement for all cases was higher for LM (94%) compared with VM (82%). Survey results indicate participants found low grade dysplasia and koilocytes easy to diagnose using three focal plane levels, the image enhancement tool was useful and focusing through the cells helped with interpretation; however, the participants found VI with hyperchromatic crowded groups challenging to interpret. Participants reported they prefer using LM over VM. This study supports using three focal plane levels and 1 μ interval to expand the use of VM in GYN cytology. Conclusion: Future improvements in technology and appropriate training should make this format a more preferable and practical option in clinical cytology. |
| first_indexed | 2024-12-11T18:14:38Z |
| format | Article |
| id | doaj.art-bcd4663efb404800bdf24718f9726ff7 |
| institution | Directory Open Access Journal |
| issn | 2153-3539 2153-3539 |
| language | English |
| last_indexed | 2024-12-11T18:14:38Z |
| publishDate | 2013-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pathology Informatics |
| spelling | doaj.art-bcd4663efb404800bdf24718f9726ff72022-12-22T00:55:28ZengElsevierJournal of Pathology Informatics2153-35392153-35392013-01-0141383810.4103/2153-3539.124015Optimal z-axis scanning parameters for gynecologic cytology specimensAmber D DonnellyMaheswari S MukherjeeElizabeth R LydenJulia A BridgeSubodh M LeleNajia WrightMary F McGaugheyAlicia M CulbersonAdam J HornWhitney R WedelStanley J RadioBackground: The use of virtual microscopy (VM) in clinical cytology has been limited due to the inability to focus through three dimensional (3D) cell clusters with a single focal plane (2D images). Limited information exists regarding the optimal scanning parameters for 3D scanning. Aims: The purpose of this study was to determine the optimal number of the focal plane levels and the optimal scanning interval to digitize gynecological (GYN) specimens prepared on SurePath™ glass slides while maintaining a manageable file size. Subjects and Methods: The iScanCoreo Au scanner (Ventana, AZ, USA) was used to digitize 192 SurePath™ glass slides at three focal plane levels at 1 μ interval. The digitized virtual images (VI) were annotated using BioImagene′s Image Viewer. Five participants interpreted the VI and recorded the focal plane level at which they felt confident and later interpreted the corresponding glass slide specimens using light microscopy (LM). The participants completed a survey about their experiences. Inter-rater agreement and concordance between the VI and the glass slide specimens were evaluated. Results: This study determined an overall high intra-rater diagnostic concordance between glass and VI (89-97%), however, the inter-rater agreement for all cases was higher for LM (94%) compared with VM (82%). Survey results indicate participants found low grade dysplasia and koilocytes easy to diagnose using three focal plane levels, the image enhancement tool was useful and focusing through the cells helped with interpretation; however, the participants found VI with hyperchromatic crowded groups challenging to interpret. Participants reported they prefer using LM over VM. This study supports using three focal plane levels and 1 μ interval to expand the use of VM in GYN cytology. Conclusion: Future improvements in technology and appropriate training should make this format a more preferable and practical option in clinical cytology.http://www.jpathinformatics.org/article.asp?issn=2153-3539;year=2013;volume=4;issue=1;spage=38;epage=38;aulast=DonnellyCytologydigital pathologyvirtual microscopyz-axis |
| spellingShingle | Amber D Donnelly Maheswari S Mukherjee Elizabeth R Lyden Julia A Bridge Subodh M Lele Najia Wright Mary F McGaughey Alicia M Culberson Adam J Horn Whitney R Wedel Stanley J Radio Optimal z-axis scanning parameters for gynecologic cytology specimens Journal of Pathology Informatics Cytology digital pathology virtual microscopy z-axis |
| title | Optimal z-axis scanning parameters for gynecologic cytology specimens |
| title_full | Optimal z-axis scanning parameters for gynecologic cytology specimens |
| title_fullStr | Optimal z-axis scanning parameters for gynecologic cytology specimens |
| title_full_unstemmed | Optimal z-axis scanning parameters for gynecologic cytology specimens |
| title_short | Optimal z-axis scanning parameters for gynecologic cytology specimens |
| title_sort | optimal z axis scanning parameters for gynecologic cytology specimens |
| topic | Cytology digital pathology virtual microscopy z-axis |
| url | http://www.jpathinformatics.org/article.asp?issn=2153-3539;year=2013;volume=4;issue=1;spage=38;epage=38;aulast=Donnelly |
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