Overexpression of Arabidopsis H+-pyrophosphatase improves the growth of alfalfa under long-term salinity, drought conditions and phosphate deficiency
Alfalfa planting is threatened by limited arable land, salinization, water shortage, and soil nutrient deprivation. To deal with this challenge, we previously introduced the Arabidopsis type I H+-pyrophosphatase gene AVP1 into alfalfa and found that transgenic lines exhibited enhanced tolerance to s...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2019-12-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
| Subjects: | |
| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-201904-0005_overexpression-of-arabidopsis-h-pyrophosphatase-improves-the-growth-of-alfalfa-under-long-term-salinity-droug.php |
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| author | Jia-Hao Su Tian-Hui Bai Fei Wang Ai-Ke Bao |
| author_facet | Jia-Hao Su Tian-Hui Bai Fei Wang Ai-Ke Bao |
| author_sort | Jia-Hao Su |
| collection | DOAJ |
| description | Alfalfa planting is threatened by limited arable land, salinization, water shortage, and soil nutrient deprivation. To deal with this challenge, we previously introduced the Arabidopsis type I H+-pyrophosphatase gene AVP1 into alfalfa and found that transgenic lines exhibited enhanced tolerance to short-term salinity or drought. In this study, the growth performances of two transgenic lines were further investigated under long-term salinity or drought conditions, as well as under phosphate deficiency (low-Pi). Compared with wild-type (WT) plants, the transgenic alfalfa showed better growth performance with taller plants and more biomass accumulation after being treated with either long-term salinity, long-term drought, or low-Pi. Most importantly, the overexpression of AVP1 significantly increased the root dry weight and the root/shoot ratio of transgenic alfalfa. A more robust root system facilitates the transgenic alfalfa to absorb nutrients, and in turn promotes the growth of the plants. Whether being treated with low-Pi or not, transgenic plants showed higher total phosphorus concentrations by 16.5-35.5% than WT plants. This study laid a foundation for breeding alfalfa cultivars adapted to saline, arid and nutrient-deprived marginal land. |
| first_indexed | 2024-04-10T08:28:46Z |
| format | Article |
| id | doaj.art-bcd547e21e99406a8732ca6dfcbc5f96 |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:28:46Z |
| publishDate | 2019-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-bcd547e21e99406a8732ca6dfcbc5f962023-02-23T03:30:46ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252019-12-0155415616110.17221/134/2018-CJGPBcjg-201904-0005Overexpression of Arabidopsis H+-pyrophosphatase improves the growth of alfalfa under long-term salinity, drought conditions and phosphate deficiencyJia-Hao SuTian-Hui BaiFei Wang0Ai-Ke BaoState Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou , P.R. ChinaAlfalfa planting is threatened by limited arable land, salinization, water shortage, and soil nutrient deprivation. To deal with this challenge, we previously introduced the Arabidopsis type I H+-pyrophosphatase gene AVP1 into alfalfa and found that transgenic lines exhibited enhanced tolerance to short-term salinity or drought. In this study, the growth performances of two transgenic lines were further investigated under long-term salinity or drought conditions, as well as under phosphate deficiency (low-Pi). Compared with wild-type (WT) plants, the transgenic alfalfa showed better growth performance with taller plants and more biomass accumulation after being treated with either long-term salinity, long-term drought, or low-Pi. Most importantly, the overexpression of AVP1 significantly increased the root dry weight and the root/shoot ratio of transgenic alfalfa. A more robust root system facilitates the transgenic alfalfa to absorb nutrients, and in turn promotes the growth of the plants. Whether being treated with low-Pi or not, transgenic plants showed higher total phosphorus concentrations by 16.5-35.5% than WT plants. This study laid a foundation for breeding alfalfa cultivars adapted to saline, arid and nutrient-deprived marginal land.https://cjgpb.agriculturejournals.cz/artkey/cjg-201904-0005_overexpression-of-arabidopsis-h-pyrophosphatase-improves-the-growth-of-alfalfa-under-long-term-salinity-droug.phpavp1 genegrowth performancephosphorus nutritionstress tolerancetransgenic alfalfa |
| spellingShingle | Jia-Hao Su Tian-Hui Bai Fei Wang Ai-Ke Bao Overexpression of Arabidopsis H+-pyrophosphatase improves the growth of alfalfa under long-term salinity, drought conditions and phosphate deficiency Czech Journal of Genetics and Plant Breeding avp1 gene growth performance phosphorus nutrition stress tolerance transgenic alfalfa |
| title | Overexpression of Arabidopsis H+-pyrophosphatase improves the growth of alfalfa under long-term salinity, drought conditions and phosphate deficiency |
| title_full | Overexpression of Arabidopsis H+-pyrophosphatase improves the growth of alfalfa under long-term salinity, drought conditions and phosphate deficiency |
| title_fullStr | Overexpression of Arabidopsis H+-pyrophosphatase improves the growth of alfalfa under long-term salinity, drought conditions and phosphate deficiency |
| title_full_unstemmed | Overexpression of Arabidopsis H+-pyrophosphatase improves the growth of alfalfa under long-term salinity, drought conditions and phosphate deficiency |
| title_short | Overexpression of Arabidopsis H+-pyrophosphatase improves the growth of alfalfa under long-term salinity, drought conditions and phosphate deficiency |
| title_sort | overexpression of arabidopsis h pyrophosphatase improves the growth of alfalfa under long term salinity drought conditions and phosphate deficiency |
| topic | avp1 gene growth performance phosphorus nutrition stress tolerance transgenic alfalfa |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-201904-0005_overexpression-of-arabidopsis-h-pyrophosphatase-improves-the-growth-of-alfalfa-under-long-term-salinity-droug.php |
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